Construct: sgRNA BRDN0001144894
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCCGGCTTATACTCCCAGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LIMK1 (3984)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76047
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
74106204 |
+ |
LIMK1 |
NNNGGCTTATACTCCCAGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
50261310 |
+ |
GNAI2 |
NNNGGCTCATGCTCCCAGCG |
NGG |
2 |
0.65 |
Tier II |
| 3 |
NC_000019.10 |
19 |
40351773 |
+ |
PLD3 |
NNNGGCTTTTACTCCCAGTG |
NGG |
2 |
0.2769 |
Tier II |
| 4 |
NC_000023.11 |
X |
119665004 |
+ |
SEPTIN6 |
NNNGGCTTATCCTCCCAGCC |
NGG |
2 |
0.1714 |
Tier II |
| 5 |
NC_000017.11 |
17 |
38533940 |
+ |
SRCIN1 |
NNNGGCTTATCCTCCCAGGG |
NGG |
2 |
0.05 |
Tier II |
| 6 |
NC_000005.10 |
5 |
10083801 |
+ |
LOC124900939 |
NNNGGGTTATTCTCCCAGCG |
NGG |
2 |
0.1538 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
87416639 |
+ |
Gigyf2 |
NNNGGCTTAAACTCCCAGCT |
NGG |
2 |
0.6 |
Tier II |
| 2 |
NC_000072.6 |
6 |
34761800 |
+ |
Cald1 |
NNNGGCTGCTACTCCCAGCG |
NGG |
2 |
0.419 |
Tier II |
| 3 |
NC_000070.6 |
4 |
123121456 |
- |
Bmp8b |
NNNGGCTTATTCTCCCAGCA |
NGG |
2 |
0.2885 |
Tier II |
| 4 |
NC_000070.6 |
4 |
123317698 |
+ |
Bmp8a |
NNNGGCTTATTCTCCCAGCA |
NGG |
2 |
0.2885 |
Tier II |
| 5 |
NC_000076.6 |
10 |
60201914 |
- |
Chst3 |
NNNTGCTTATAATCCCAGCG |
NGG |
2 |
0.2597 |
Tier II |
| 6 |
NC_000077.6 |
11 |
23406751 |
- |
Usp34 |
NNNGGCTTCTACTCCCAGCC |
NGG |
2 |
0.2449 |
Tier II |
| 7 |
NC_000075.6 |
9 |
91369326 |
- |
Zic4 |
NNNGGCCTATTCTCCCAGCG |
NGG |
2 |
0.2115 |
Tier II |
| 8 |
NC_000067.6 |
1 |
136548317 |
- |
Kif14 |
NNNTGCTTATACTCCCAGCC |
NGG |
2 |
0.1558 |
Tier II |
| 9 |
NC_000071.6 |
5 |
134049888 |
+ |
Gm40339 |
NNNGGCTTATACTTCCAGAG |
NGG |
2 |
0.3143 |
Tier III |
| 10 |
NC_000081.6 |
15 |
79983014 |
+ |
Gm52191 |
NNNGGCTTATAGTCCCAGAG |
NGG |
2 |
0.1905 |
Tier III |
| 11 |
NC_000069.6 |
3 |
38702993 |
- |
Gm29879 |
NNNGTCTTATACTCCCAGAG |
NGG |
2 |
0.1286 |
Tier III |
Other clones with same target sequence:
(none)
