Construct: sgRNA BRDN0001144895

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TATGGGAGACCTACCAGAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK1D (57118)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77443

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 12814305 - CAMK1D NNNGGGAGACCTACCAGAGT NGG 0 1.0 Tier I
2 NC_000015.10 15 48760215 - CEP152 NNNGGGAGAACTACCAGAGC NGG 2 0.0788 Tier I
3 NC_000001.11 1 15651791 + DDI2 NNNGGGAGAGCTACCAGAGT NTG 2 0.0152 Tier I
4 NC_000012.12 12 53224592 - RARG NNNGGGAAACCTTCCAGAGT NGG 2 0.3 Tier II
5 NC_000022.11 22 31633968 - PISD NNNGGGAGTCCTAACAGAGT NGG 2 0.21 Tier II
6 NC_000019.10 19 4901858 - ARRDC5 NNNGGGAGACCTTCCAGAGA NGG 2 0.1687 Tier II
7 NC_000012.12 12 100504955 + NR1H4 NNNGGCAGACCTCCCAGAGT NGG 2 0.1435 Tier II
8 NC_000012.12 12 3532961 + PRMT8 NNNGGGAGGCCTCCCAGAGT NGG 2 0.1404 Tier II
9 NC_000015.10 15 33744615 + RYR3 NNNGGGACACCTCCCAGAGT NGG 2 0.1296 Tier II
10 NC_000009.12 9 5114432 + JAK2 NNNGGGAGACCTACCAGTGC NGG 2 0.0455 Tier II
11 NC_000009.12 9 5114432 + INSL6 NNNGGGAGACCTACCAGTGC NGG 2 0.0455 Tier II
12 NC_000002.12 2 73269893 + FBXO41 NNNGGGAGACCTCCCAGAGG NGG 2 0.0372 Tier II
13 NC_000003.12 3 89150504 + EPHA3 NNNGGGAGACCTTCTAGAGT NGG 2 0.02 Tier II
14 NC_000017.11 17 18388458 + EVPLL NNNGGGAGACCCAGCAGAGT NGG 2 0.0 Tier II
15 NC_000012.12 12 53341174 - SP7 NNNGGGAGACCTAGCAGAGA NGG 2 0.0 Tier II
16 NC_000002.12 2 217536778 + DIRC3 NNNGGCAGCCCTACCAGAGT NGG 2 0.3896 Tier III
17 NC_000007.14 7 26617783 - LOC101928077 NNNGGGAGACCTCCCAGTGT NGG 2 0.1053 Tier III
18 NC_000022.11 22 34107779 - LINC01643 NNNGGGAGACCCACCAGAGC NGG 2 0.0861 Tier III
19 NC_000009.12 9 5114432 + IGHEP2 NNNGGGAGACCTACCAGTGC NGG 2 0.0455 Tier III
20 NC_000014.9 14 105600542 - IGH NNNGGGAGACCTACCAGTGC NGG 2 0.0455 Tier III
21 NC_000014.9 14 105600542 - IGHE NNNGGGAGACCTACCAGTGC NGG 2 0.0455 Tier III
22 NC_000014.9 14 105722270 - IGH NNNGGGAGACCTACCAGTGC NGG 2 0.0455 Tier III
23 NC_000014.9 14 105722270 - IGHEP1 NNNGGGAGACCTACCAGTGC NGG 2 0.0455 Tier III
24 NC_000001.11 1 193520477 - LOC124904475 NNNGGGAGACCTACCAGTGT NGT 2 0.0081 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 74375819 + Slc11a1 NNNGGGAGACCTACCTGAGT NAG 2 0.0 Tier I
2 NC_000086.7 X 36851672 + C330007P06Rik NNNAGGAGACCAACCAGAGT NGG 2 0.72 Tier II
3 NC_000078.6 12 73743252 - Prkch NNNGGGAGACCAATCAGAGT NGG 2 0.5867 Tier II
4 NC_000073.6 7 73941870 + St8sia2 NNNGGGAGGCCTAACAGAGT NGG 2 0.2333 Tier II
5 NC_000080.6 14 12171815 + Ptprg NNNTGGAGACCTACCAGGGT NGG 2 0.1455 Tier II
6 NC_000072.6 6 125051375 + Acrbp NNNGTGAGACCTCCCAGAGT NGG 2 0.0632 Tier II
7 NC_000073.6 7 122392464 - Prkcb NNNGGCAGACCTACCAGAGT NTG 2 0.0266 Tier II
8 NC_000074.6 8 54892145 - Gpm6a NNNGGGAGACCTAGCAGAGT NGG 1 0.0 Tier II
9 NC_000078.6 12 53716562 + Npas3 NNNGGGAGACCTAGCATAGT NGG 2 0.0 Tier II
10 NC_000081.6 15 52744512 + Gm41323 NNNGGGAGACCCAGCAGAGT NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)