Construct: sgRNA BRDN0001144900
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAGCATTCCATCATTCCGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TRRAP (8295)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76537
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
98899436 |
+ |
TRRAP |
NNNCATTCCATCATTCCGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000018.10 |
18 |
58384838 |
- |
NEDD4L |
NNNCATTCCATCATTCTGAA |
NGG |
2 |
0.4375 |
Tier II |
| 3 |
NC_000022.11 |
22 |
17584971 |
- |
SLC25A18 |
NNNCACTCCATCATTCTGAG |
NGG |
2 |
0.4242 |
Tier II |
| 4 |
NC_000015.10 |
15 |
59356296 |
+ |
MYO1E |
NNNCATTCCATCTTTCCGAA |
NGG |
2 |
0.2813 |
Tier II |
| 5 |
NC_000009.12 |
9 |
37255055 |
+ |
ZCCHC7 |
NNNCATTCCAGCATTCCCAG |
NGG |
2 |
0.1905 |
Tier II |
| 6 |
NC_000015.10 |
15 |
81903968 |
- |
LINC01418 |
NNNCATTTCATCATTCCGAA |
NGG |
2 |
0.8203 |
Tier III |
| 7 |
NC_000022.11 |
22 |
17584971 |
- |
LOC101929372 |
NNNCACTCCATCATTCTGAG |
NGG |
2 |
0.4242 |
Tier III |
| 8 |
NC_000006.12 |
6 |
93602952 |
- |
LOC105377899 |
NNNCATTCCAACATTCCCAG |
NGG |
2 |
0.3571 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
90812661 |
- |
Iqsec1 |
NNNCATCCCAACATTCCGAG |
NGG |
2 |
0.5156 |
Tier II |
| 2 |
NC_000074.6 |
8 |
18738996 |
+ |
Angpt2 |
NNNCATGCAATCATTCCGAG |
NGG |
2 |
0.5042 |
Tier II |
| 3 |
NC_000074.6 |
8 |
18738996 |
+ |
Mcph1 |
NNNCATGCAATCATTCCGAG |
NGG |
2 |
0.5042 |
Tier II |
| 4 |
NC_000067.6 |
1 |
154419118 |
- |
Cacna1e |
NNNCTTTCCATCATTCCAAG |
NGG |
2 |
0.2517 |
Tier II |
Other clones with same target sequence:
(none)
