Construct: sgRNA BRDN0001144903
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCCCCACAGAGGGCGAACAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BCR (613)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76909
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
23287167 |
+ |
BCR |
NNNCCACAGAGGGCGAACAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
81297054 |
+ |
IL16 |
NNNCCACAGAGGGCGAGCCA |
NGG |
2 |
0.0365 |
Tier I |
| 3 |
NC_000006.12 |
6 |
138096421 |
+ |
PERP |
NNNCCACAGAGGGCGAAGAA |
NGA |
2 |
0.0093 |
Tier I |
| 4 |
NC_000012.12 |
12 |
23638013 |
+ |
SOX5 |
NNNGCACAGAGGGTGAACAA |
NGG |
2 |
0.3667 |
Tier II |
| 5 |
NC_000022.11 |
22 |
46061609 |
- |
LOC124905135 |
NNNCCATTGAGGGCGAACAA |
NGG |
2 |
0.3482 |
Tier II |
| 6 |
NC_000023.11 |
X |
1307038 |
+ |
CSF2RA |
NNNCCAGAGAGGGTGAACAA |
NGG |
2 |
0.3451 |
Tier II |
| 7 |
NC_000024.10 |
Y |
1307038 |
+ |
CSF2RA |
NNNCCAGAGAGGGTGAACAA |
NGG |
2 |
0.3451 |
Tier II |
| 8 |
NC_000019.10 |
19 |
7631873 |
+ |
STXBP2 |
NNNCCACAGGTGGCGAACAA |
NGG |
2 |
0.2137 |
Tier II |
| 9 |
NC_000019.10 |
19 |
7631873 |
+ |
PCP2 |
NNNCCACAGGTGGCGAACAA |
NGG |
2 |
0.2137 |
Tier II |
| 10 |
NC_000019.10 |
19 |
7631873 |
+ |
PET100 |
NNNCCACAGGTGGCGAACAA |
NGG |
2 |
0.2137 |
Tier II |
| 11 |
NC_000003.12 |
3 |
151740302 |
- |
AADACL2 |
NNNCCACAGAGGGCGAACAG |
NAG |
2 |
0.1983 |
Tier II |
| 12 |
NC_000006.12 |
6 |
79677969 |
- |
SH3BGRL2 |
NNNCCACAGAGTGAGAACAA |
NGG |
2 |
0.1346 |
Tier II |
| 13 |
NC_000012.12 |
12 |
13948353 |
- |
GRIN2B |
NNNCCCCAGAGGGCTAACAA |
NGG |
2 |
0.0649 |
Tier II |
| 14 |
NC_000015.10 |
15 |
76198732 |
- |
TMEM266 |
NNNCCACAGAGGGGGACCAA |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000007.14 |
7 |
34797215 |
+ |
NPSR1 |
NNNGCACAGAGGGGGAACAA |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000022.11 |
22 |
23287167 |
+ |
LOC107985554 |
NNNCCACAGAGGGCGAACAA |
NGG |
0 |
1.0 |
Tier III |
| 17 |
NC_000019.10 |
19 |
5677777 |
- |
LOC107985320 |
NNNCCACAGAGGGCGATCCA |
NGG |
2 |
0.0276 |
Tier III |
| 18 |
NC_000005.10 |
5 |
6314095 |
- |
LINC02145 |
NNNCCACAGAGGAGGAACAA |
NGG |
2 |
0.0 |
Tier III |
| 19 |
NC_000007.14 |
7 |
34797215 |
+ |
NPSR1-AS1 |
NNNGCACAGAGGGGGAACAA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
62718237 |
+ |
Zfp287 |
NNNTCACAGAGGGTGAACAA |
NGG |
2 |
0.5867 |
Tier II |
| 2 |
NC_000077.6 |
11 |
62720351 |
- |
Zfp287 |
NNNTCACAGAGGGTGAACAA |
NGG |
2 |
0.5867 |
Tier II |
| 3 |
NC_000071.6 |
5 |
103881927 |
+ |
Klhl8 |
NNNCAACGGAGGGCGAACAA |
NGG |
2 |
0.419 |
Tier II |
| 4 |
NC_000075.6 |
9 |
99020803 |
- |
Gm1123 |
NNNCCACAGAGGCCAAACAA |
NGG |
2 |
0.3963 |
Tier II |
| 5 |
NC_000072.6 |
6 |
148120841 |
- |
Far2 |
NNNCCACAGAGGGCCAACAG |
NGG |
2 |
0.2086 |
Tier II |
| 6 |
NC_000074.6 |
8 |
54840889 |
- |
Gpm6a |
NNNCCTCAGAGGGCTAACAA |
NGG |
2 |
0.102 |
Tier II |
| 7 |
NC_000068.7 |
2 |
153291586 |
- |
Kif3b |
NNNCCACAGAGGCCGGACAA |
NGG |
2 |
0.081 |
Tier II |
| 8 |
NC_000074.6 |
8 |
46040632 |
- |
Snx25 |
NNNCCACAGAGGGTGAACAA |
NGA |
2 |
0.0509 |
Tier II |
| 9 |
NC_000075.6 |
9 |
116797229 |
+ |
Rbms3 |
NNNCCACAGAGGGCTAAGAA |
NGG |
2 |
0.019 |
Tier II |
| 10 |
NC_000067.6 |
1 |
93030007 |
+ |
Kif1a |
NNNCCACAGAGGGCAAACAA |
NGT |
2 |
0.0152 |
Tier II |
| 11 |
NC_000076.6 |
10 |
87558881 |
+ |
Pah |
NNNCCACTGAGGGCGAACAA |
NGC |
2 |
0.0095 |
Tier II |
| 12 |
NC_000069.6 |
3 |
60868545 |
- |
Gm553061 |
NNNCCACCGAGGGCTAACAA |
NGG |
2 |
0.0612 |
Tier III |
Other clones with same target sequence:
(none)
