Construct: sgRNA BRDN0001144909
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGCTGGCATCATAACAACC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIKFYVE (200576)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76890
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
208314369 |
+ |
PIKFYVE |
NNNCTGGCATCATAACAACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
7801381 |
- |
DNAH2 |
NNNCTGGCATCAGAAAAACC |
NGG |
2 |
0.2609 |
Tier II |
| 3 |
NC_000010.11 |
10 |
18291024 |
- |
CACNB2 |
NNNCTGGTATCCTAACAACC |
NGG |
2 |
0.2303 |
Tier II |
| 4 |
NC_000009.12 |
9 |
130321231 |
- |
HMCN2 |
NNNCTGGAATCATAAGAACC |
NGG |
2 |
0.1 |
Tier II |
| 5 |
NC_000011.10 |
11 |
96313918 |
- |
MAML2 |
NNNCTGGCATCAGAACCACC |
NGG |
2 |
0.046 |
Tier II |
| 6 |
NC_000008.11 |
8 |
3763229 |
- |
CSMD1 |
NNNCTGGCATCATAACATCG |
NGG |
2 |
0.0294 |
Tier II |
| 7 |
NC_000008.11 |
8 |
11198892 |
+ |
XKR6 |
NNNCTGGCATCTTAACAACC |
NGA |
2 |
0.0231 |
Tier II |
| 8 |
NC_000005.10 |
5 |
137247814 |
+ |
SPOCK1 |
NNNCTGGCTTCATAACAACC |
NGC |
2 |
0.0133 |
Tier II |
| 9 |
NC_000001.11 |
1 |
224134487 |
- |
FBXO28 |
NNNCTGGCATCATAACCACC |
NGT |
2 |
0.0028 |
Tier II |
| 10 |
NC_000006.12 |
6 |
87846149 |
+ |
LOC101928911 |
NNNTTGGCATCACAACAACC |
NGG |
2 |
0.6316 |
Tier III |
| 11 |
NC_000003.12 |
3 |
178049817 |
- |
LOC105374234 |
NNNGTGGCATCATAACAACC |
NAG |
2 |
0.1296 |
Tier III |
| 12 |
NC_000009.12 |
9 |
1333623 |
- |
LOC102723803 |
NNNGTGGCATCATAAGAACC |
NGG |
2 |
0.0769 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
65233805 |
+ |
Pikfyve |
NNNCTGGCATCATAACAACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000069.6 |
3 |
107602717 |
+ |
Alx3 |
NNNCTGGTATCACAACAACC |
NGG |
2 |
0.6908 |
Tier II |
| 3 |
NC_000068.7 |
2 |
146073741 |
+ |
Cfap61 |
NNNATGCCATCATAACAACC |
NGG |
2 |
0.5789 |
Tier II |
| 4 |
NC_000078.6 |
12 |
112963232 |
+ |
Brf1 |
NNNCTTGCAACATAACAACC |
NGG |
2 |
0.5714 |
Tier II |
| 5 |
NC_000085.6 |
19 |
32167263 |
- |
Sgms1 |
NNNCTCCCATCATAACAACC |
NGG |
2 |
0.4687 |
Tier II |
| 6 |
NC_000072.6 |
6 |
23313772 |
+ |
Cadps2 |
NNNATGGCATCATAACAAAC |
NGG |
2 |
0.3609 |
Tier II |
| 7 |
NC_000078.6 |
12 |
89566940 |
+ |
Nrxn3 |
NNNCTGGCATAATGACAACC |
NGG |
2 |
0.35 |
Tier II |
| 8 |
NC_000079.6 |
13 |
117960427 |
- |
Hcn1 |
NNNCTAGCATCATAACAACC |
NAG |
2 |
0.2593 |
Tier II |
| 9 |
NC_000081.6 |
15 |
66811785 |
- |
Sla |
NNNCTGGCATCAGAACAATC |
NGG |
2 |
0.1204 |
Tier II |
| 10 |
NC_000081.6 |
15 |
66811785 |
- |
Tg |
NNNCTGGCATCAGAACAATC |
NGG |
2 |
0.1204 |
Tier II |
| 11 |
NC_000080.6 |
14 |
16833778 |
+ |
Rarb |
NNNCTGGAATCATAAGAACC |
NGG |
2 |
0.1 |
Tier II |
| 12 |
NC_000069.6 |
3 |
156789966 |
- |
Negr1 |
NNNCTGTCATCATAACAACC |
NGA |
2 |
0.0397 |
Tier II |
| 13 |
NC_000079.6 |
13 |
94193308 |
+ |
Lhfpl2 |
NNNCTGGCATCATAAGAACC |
NTG |
2 |
0.006 |
Tier II |
| 14 |
NC_000079.6 |
13 |
54935366 |
+ |
Gm31313 |
NNNCTGGCTTCATAAGAACC |
NGG |
2 |
0.0923 |
Tier III |
| 15 |
NC_000075.6 |
9 |
43517551 |
- |
Gm36855 |
NNNCTGGCATCATATCAGCC |
NGG |
2 |
0.08 |
Tier III |
Other clones with same target sequence:
(none)
