Construct: sgRNA BRDN0001144917
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAGGATCGAGATGGTGCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CARD11 (84433)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77680
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
2937112 |
- |
CARD11 |
NNNGGATCGAGATGGTGCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000021.9 |
21 |
26966073 |
- |
ADAMTS5 |
NNNGGAGCGAGATGGTTCGG |
NGG |
2 |
0.1471 |
Tier I |
| 3 |
NC_000020.11 |
20 |
58701958 |
- |
NPEPL1 |
NNNGGATGGAGATGGTGCGG |
NGG |
1 |
0.6429 |
Tier II |
| 4 |
NC_000001.11 |
1 |
14138701 |
- |
KAZN |
NNNGGATGGAGATGGTGCGA |
NGG |
2 |
0.6027 |
Tier II |
| 5 |
NC_000002.12 |
2 |
31051208 |
+ |
GALNT14 |
NNNGGATCAAGATGGTGCAG |
NGG |
2 |
0.4592 |
Tier II |
| 6 |
NC_000011.10 |
11 |
124880737 |
+ |
ROBO3 |
NNNGGATCGAGAGGGTGAGG |
NGG |
2 |
0.1405 |
Tier II |
| 7 |
NC_000021.9 |
21 |
6544405 |
+ |
LOC102724652 |
NNNGGATCGAGGTGGTGGGG |
NGG |
2 |
0.0963 |
Tier II |
| 8 |
NC_000020.11 |
20 |
58701958 |
- |
STX16-NPEPL1 |
NNNGGATGGAGATGGTGCGG |
NGG |
1 |
0.6429 |
Tier III |
| 9 |
NC_000021.9 |
21 |
6544405 |
+ |
LOC102724701 |
NNNGGATCGAGGTGGTGGGG |
NGG |
2 |
0.0963 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
128655288 |
+ |
Phc2 |
NNNAGATCGAGATGGTGCGT |
NGG |
2 |
0.63 |
Tier II |
| 2 |
NC_000081.6 |
15 |
50887748 |
+ |
Trps1 |
NNNGGATCGAGATGCTACGG |
NGG |
2 |
0.2545 |
Tier II |
| 3 |
NC_000083.6 |
17 |
84604556 |
+ |
Plekhh2 |
NNNGGATGGAGATGGGGCGG |
NGG |
2 |
0.2225 |
Tier II |
| 4 |
NC_000071.6 |
5 |
128410365 |
+ |
Tmem132d |
NNNGGATGGAGATGGTTCGG |
NGG |
2 |
0.1607 |
Tier II |
Other clones with same target sequence:
(none)
