Construct: sgRNA BRDN0001144918
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGATAGGTCTTATTTACGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GUCY2C (2984)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77566
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
14669803 |
+ |
GUCY2C |
NNNATAGGTCTTATTTACGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
40119279 |
+ |
SLC8A1 |
NNNATAGGTTTTATTTACTT |
NGG |
2 |
0.6275 |
Tier II |
| 3 |
NC_000007.14 |
7 |
132268578 |
+ |
PLXNA4 |
NNNATGGGTCTTATTTACAT |
NGG |
2 |
0.5102 |
Tier II |
| 4 |
NC_000013.11 |
13 |
98872224 |
- |
DOCK9 |
NNNAAAAGTCTTATTTACGT |
NGG |
2 |
0.5 |
Tier II |
| 5 |
NC_000009.12 |
9 |
98191676 |
+ |
CORO2A |
NNNATAGGTCTTCTTTACCT |
NGG |
2 |
0.0944 |
Tier II |
| 6 |
NC_000021.9 |
21 |
18295549 |
- |
TMPRSS15 |
NNNTTAGGTCTTATTTACGT |
NGC |
2 |
0.0141 |
Tier II |
| 7 |
NC_000012.12 |
12 |
14669803 |
+ |
GUCY2C-AS1 |
NNNATAGGTCTTATTTACGT |
NGG |
0 |
1.0 |
Tier III |
| 8 |
NC_000002.12 |
2 |
40119279 |
+ |
SLC8A1-AS1 |
NNNATAGGTTTTATTTACTT |
NGG |
2 |
0.6275 |
Tier III |
| 9 |
NC_000006.12 |
6 |
78161893 |
+ |
LOC105377865 |
NNNAGAGGTCTTATTTACAT |
NGG |
2 |
0.4571 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
57613485 |
+ |
Pakap |
NNNATAGGTCATATTTAAGT |
NGG |
2 |
0.4038 |
Tier II |
| 2 |
NC_000071.6 |
5 |
143418251 |
- |
Kdelr2 |
NNNATAGGTCTTTTTTACGC |
NGG |
2 |
0.0273 |
Tier II |
| 3 |
NC_000071.6 |
5 |
93140408 |
+ |
Septin11 |
NNNATAGGTCTTATTCACGT |
NGT |
2 |
0.0108 |
Tier II |
| 4 |
NC_000072.6 |
6 |
149441009 |
+ |
Bicd1 |
NNNATAGGTCTTATTTACGT |
NTC |
2 |
0.0 |
Tier II |
| 5 |
NC_000073.6 |
7 |
59950515 |
- |
Snhg14 |
NNNATAGGTCTTATTTAGGT |
NGT |
2 |
0.0022 |
Tier III |
Other clones with same target sequence:
(none)
