Construct: sgRNA BRDN0001144922
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAACCACAAGATTTGGACC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NRK (203447)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76315
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
105909182 |
+ |
NRK |
NNNACCACAAGATTTGGACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
31300871 |
- |
B3GLCT |
NNNACCACAAGATTAGGATC |
NGG |
2 |
0.2672 |
Tier II |
| 3 |
NC_000005.10 |
5 |
168161183 |
+ |
TENM2 |
NNNTCCACAAGATTTGGGCC |
NGG |
2 |
0.2545 |
Tier II |
| 4 |
NC_000010.11 |
10 |
79137695 |
+ |
ZMIZ1 |
NNNACCACAAGATTTGCACC |
NGG |
1 |
0.2353 |
Tier II |
| 5 |
NC_000010.11 |
10 |
66908192 |
+ |
CTNNA3 |
NNNACCAAAAGAGTTGGACC |
NGG |
2 |
0.1696 |
Tier II |
| 6 |
NC_000002.12 |
2 |
157533191 |
+ |
ACVR1C |
NNNATCACAAGATTTGGACC |
NAG |
2 |
0.165 |
Tier II |
| 7 |
NC_000017.11 |
17 |
2232446 |
+ |
SMG6 |
NNNACCACCAGCTTTGGACC |
NGG |
2 |
0.1504 |
Tier II |
| 8 |
NC_000005.10 |
5 |
131432293 |
- |
RAPGEF6 |
NNNACCACCAGATTTGGCCC |
NGG |
2 |
0.1088 |
Tier II |
| 9 |
NC_000017.11 |
17 |
65879479 |
- |
CEP112 |
NNNACCACAAGCTTTGTACC |
NGG |
2 |
0.0658 |
Tier II |
| 10 |
NC_000021.9 |
21 |
33846441 |
- |
ITSN1 |
NNNACCACAAGATTTGGGCC |
NTG |
2 |
0.0156 |
Tier II |
| 11 |
NC_000004.12 |
4 |
61426232 |
+ |
ADGRL3 |
NNNACCACAAGATTGGGCCC |
NGG |
2 |
0.0095 |
Tier II |
| 12 |
NC_000015.10 |
15 |
45774151 |
+ |
LOC105370802 |
NNNACCATAAGATTTGGACA |
NGG |
2 |
0.4375 |
Tier III |
| 13 |
NC_000005.10 |
5 |
104694779 |
+ |
LOC105379109 |
NNNAACACAAGATTTGGACA |
NGG |
2 |
0.2857 |
Tier III |
| 14 |
NC_000010.11 |
10 |
66908192 |
+ |
LOC101928961 |
NNNACCAAAAGAGTTGGACC |
NGG |
2 |
0.1696 |
Tier III |
| 15 |
NC_000017.11 |
17 |
65879479 |
- |
LOC105371867 |
NNNACCACAAGCTTTGTACC |
NGG |
2 |
0.0658 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
38937280 |
- |
Hells |
NNNACCACAAGATTTGAACA |
NGG |
2 |
0.4667 |
Tier II |
| 2 |
NC_000073.6 |
7 |
92768871 |
- |
Rab30 |
NNNACCACAAGACTTGGACA |
NGG |
2 |
0.3947 |
Tier II |
| 3 |
NC_000076.6 |
10 |
26779185 |
- |
Arhgap18 |
NNNAACAGAAGATTTGGACC |
NGG |
2 |
0.3673 |
Tier II |
| 4 |
NC_000073.6 |
7 |
109039264 |
- |
Ric3 |
NNNACCAGAAGATTTGGAAC |
NGG |
2 |
0.2755 |
Tier II |
| 5 |
NC_000075.6 |
9 |
99220102 |
+ |
Cep70 |
NNNACCACAAGACTTGGACT |
NGG |
2 |
0.2368 |
Tier II |
| 6 |
NC_000076.6 |
10 |
102120926 |
- |
Mgat4c |
NNNACCACAAGATATGTACC |
NGG |
2 |
0.1548 |
Tier II |
| 7 |
NC_000067.6 |
1 |
37041386 |
- |
Vwa3b |
NNNATCACAAGATTTGGCCC |
NGG |
2 |
0.1212 |
Tier II |
| 8 |
NC_000085.6 |
19 |
43200170 |
+ |
Hpse2 |
NNNACCACAACATTTGGCCC |
NGG |
2 |
0.0816 |
Tier II |
| 9 |
NC_000071.6 |
5 |
23139737 |
- |
Lhfpl3 |
NNNACCAGAAGATTGGGACC |
NGG |
2 |
0.0321 |
Tier II |
| 10 |
NC_000071.6 |
5 |
13143284 |
+ |
Sema3a |
NNNACCACAAGGTTTGGACC |
NTG |
2 |
0.0281 |
Tier II |
| 11 |
NC_000081.6 |
15 |
71701008 |
+ |
Fam135b |
NNNATCACAAGATTTGGACC |
NTG |
2 |
0.0248 |
Tier II |
| 12 |
NC_000075.6 |
9 |
99220102 |
+ |
Gm2950 |
NNNACCACAAGACTTGGACT |
NGG |
2 |
0.2368 |
Tier III |
| 13 |
NC_000071.6 |
5 |
13143284 |
+ |
Gm52804 |
NNNACCACAAGGTTTGGACC |
NTG |
2 |
0.0281 |
Tier III |
Other clones with same target sequence:
(none)
