Construct: sgRNA BRDN0001144922

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGAACCACAAGATTTGGACC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NRK (203447)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76315

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 105909182 + NRK NNNACCACAAGATTTGGACC NGG 0 1.0 Tier I
2 NC_000013.11 13 31300871 - B3GLCT NNNACCACAAGATTAGGATC NGG 2 0.2672 Tier II
3 NC_000005.10 5 168161183 + TENM2 NNNTCCACAAGATTTGGGCC NGG 2 0.2545 Tier II
4 NC_000010.11 10 79137695 + ZMIZ1 NNNACCACAAGATTTGCACC NGG 1 0.2353 Tier II
5 NC_000010.11 10 66908192 + CTNNA3 NNNACCAAAAGAGTTGGACC NGG 2 0.1696 Tier II
6 NC_000002.12 2 157533191 + ACVR1C NNNATCACAAGATTTGGACC NAG 2 0.165 Tier II
7 NC_000017.11 17 2232446 + SMG6 NNNACCACCAGCTTTGGACC NGG 2 0.1504 Tier II
8 NC_000005.10 5 131432293 - RAPGEF6 NNNACCACCAGATTTGGCCC NGG 2 0.1088 Tier II
9 NC_000017.11 17 65879479 - CEP112 NNNACCACAAGCTTTGTACC NGG 2 0.0658 Tier II
10 NC_000021.9 21 33846441 - ITSN1 NNNACCACAAGATTTGGGCC NTG 2 0.0156 Tier II
11 NC_000004.12 4 61426232 + ADGRL3 NNNACCACAAGATTGGGCCC NGG 2 0.0095 Tier II
12 NC_000015.10 15 45774151 + LOC105370802 NNNACCATAAGATTTGGACA NGG 2 0.4375 Tier III
13 NC_000005.10 5 104694779 + LOC105379109 NNNAACACAAGATTTGGACA NGG 2 0.2857 Tier III
14 NC_000010.11 10 66908192 + LOC101928961 NNNACCAAAAGAGTTGGACC NGG 2 0.1696 Tier III
15 NC_000017.11 17 65879479 - LOC105371867 NNNACCACAAGCTTTGTACC NGG 2 0.0658 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000085.6 19 38937280 - Hells NNNACCACAAGATTTGAACA NGG 2 0.4667 Tier II
2 NC_000073.6 7 92768871 - Rab30 NNNACCACAAGACTTGGACA NGG 2 0.3947 Tier II
3 NC_000076.6 10 26779185 - Arhgap18 NNNAACAGAAGATTTGGACC NGG 2 0.3673 Tier II
4 NC_000073.6 7 109039264 - Ric3 NNNACCAGAAGATTTGGAAC NGG 2 0.2755 Tier II
5 NC_000075.6 9 99220102 + Cep70 NNNACCACAAGACTTGGACT NGG 2 0.2368 Tier II
6 NC_000076.6 10 102120926 - Mgat4c NNNACCACAAGATATGTACC NGG 2 0.1548 Tier II
7 NC_000067.6 1 37041386 - Vwa3b NNNATCACAAGATTTGGCCC NGG 2 0.1212 Tier II
8 NC_000085.6 19 43200170 + Hpse2 NNNACCACAACATTTGGCCC NGG 2 0.0816 Tier II
9 NC_000071.6 5 23139737 - Lhfpl3 NNNACCAGAAGATTGGGACC NGG 2 0.0321 Tier II
10 NC_000071.6 5 13143284 + Sema3a NNNACCACAAGGTTTGGACC NTG 2 0.0281 Tier II
11 NC_000081.6 15 71701008 + Fam135b NNNATCACAAGATTTGGACC NTG 2 0.0248 Tier II
12 NC_000075.6 9 99220102 + Gm2950 NNNACCACAAGACTTGGACT NGG 2 0.2368 Tier III
13 NC_000071.6 5 13143284 + Gm52804 NNNACCACAAGGTTTGGACC NTG 2 0.0281 Tier III
Download CSV

Other clones with same target sequence:

(none)

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