Construct: sgRNA BRDN0001144926
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTTGGTCAATATGACAACCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKAR2A (5576)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77674
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
48773046 |
- |
PRKAR2A |
NNNGGTCAATATGACAACCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
188365331 |
+ |
LPP |
NNNGGTCATTATGACAACAG |
NGG |
2 |
0.2571 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
108733173 |
+ |
Prkar2a |
NNNGGTCAGTATGACAACCG |
NGG |
1 |
0.6667 |
Tier I |
| 2 |
NC_000067.6 |
1 |
181734939 |
- |
Dnah14 |
NNNGGTCACCATGACAACCG |
NGG |
2 |
0.3048 |
Tier I |
| 3 |
NC_000073.6 |
7 |
35439751 |
- |
Cep89 |
NNNGGTCAACATGAAAACCG |
NGG |
2 |
0.1185 |
Tier II |
| 4 |
NC_000068.7 |
2 |
117943011 |
+ |
Gm13986 |
NNNCGTCAATATGACAACTG |
NGG |
2 |
0.2443 |
Tier III |
| 5 |
NC_000079.6 |
13 |
64319087 |
+ |
Gm31128 |
NNNGGTCAAGATGACAACTG |
NGG |
2 |
0.2308 |
Tier III |
| 6 |
NC_000084.6 |
18 |
47695218 |
+ |
Gm5095 |
NNNAGTCAATATGACATCCG |
NGG |
2 |
0.12 |
Tier III |
| 7 |
NC_000073.6 |
7 |
35439751 |
- |
Gm42378 |
NNNGGTCAACATGAAAACCG |
NGG |
2 |
0.1185 |
Tier III |
| 8 |
NC_000076.6 |
10 |
71172801 |
- |
C730027H18Rik |
NNNGCTCAATATGACAACCG |
NGT |
2 |
0.0127 |
Tier III |
| 9 |
NC_000070.6 |
4 |
40885815 |
- |
Gm34289 |
NNNGGTCAAAATGACCACCG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
