Construct: sgRNA BRDN0001144927
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGATCTGATGCACATACGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NUAK2 (81788)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77001
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
205311766 |
- |
NUAK2 |
NNNATCTGATGCACATACGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
88399025 |
+ |
CSNK2A2IP |
NNNATCAGATGCACATAAGG |
NGG |
2 |
0.4712 |
Tier II |
| 3 |
NC_000013.11 |
13 |
77621485 |
- |
SCEL |
NNNATCTGATGCACACATGG |
NGG |
2 |
0.4286 |
Tier II |
| 4 |
NC_000015.10 |
15 |
33487609 |
+ |
RYR3 |
NNNATCTTATGCACATATGG |
NGG |
2 |
0.4018 |
Tier II |
| 5 |
NC_000004.12 |
4 |
6006089 |
+ |
C4orf50 |
NNNATCTGATGAACATAAGG |
NGG |
2 |
0.3846 |
Tier II |
| 6 |
NC_000018.10 |
18 |
62098414 |
+ |
PIGN |
NNNATCTGTTGGACATACGG |
NGG |
2 |
0.2667 |
Tier II |
| 7 |
NC_000004.12 |
4 |
30859406 |
- |
PCDH7 |
NNNCTCTGATGCACATACAG |
NGG |
2 |
0.2521 |
Tier II |
| 8 |
NC_000003.12 |
3 |
1346809 |
+ |
CNTN6 |
NNNATCTGATGCACATGCTG |
NGG |
2 |
0.1176 |
Tier II |
| 9 |
NC_000020.11 |
20 |
7152462 |
- |
LINC01428 |
NNNATCTGATGCACATATGG |
NGA |
2 |
0.0446 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
132325013 |
+ |
Nuak2 |
NNNATCTGCTGCACATACGG |
NGG |
1 |
0.5714 |
Tier I |
| 2 |
NC_000079.6 |
13 |
51686832 |
+ |
Sema4d |
NNNATCTGATGCACATACTT |
NGG |
2 |
0.4667 |
Tier II |
| 3 |
NC_000077.6 |
11 |
32351343 |
- |
Sh3pxd2b |
NNNATCTGAAGCACATACGG |
NAG |
2 |
0.2222 |
Tier II |
| 4 |
NC_000070.6 |
4 |
156205775 |
+ |
Perm1 |
NNNACCTGATGCACATCCGG |
NGG |
2 |
0.1765 |
Tier II |
| 5 |
NC_000070.6 |
4 |
156205775 |
+ |
AW011738 |
NNNACCTGATGCACATCCGG |
NGG |
2 |
0.1765 |
Tier III |
Other clones with same target sequence:
(none)
