Construct: sgRNA BRDN0001144929
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AATTCATCTTCTACGACCCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BAZ1B (9031)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76132
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
73489269 |
+ |
BAZ1B |
NNNTCATCTTCTACGACCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
28457002 |
+ |
LINGO2 |
NNNTCATTTTCTACAACCCA |
NGG |
2 |
0.8235 |
Tier II |
| 3 |
NC_000021.9 |
21 |
41946503 |
- |
C2CD2 |
NNNTCATCTTCTATGACCCA |
NAG |
2 |
0.1901 |
Tier II |
| 4 |
NC_000002.12 |
2 |
85145788 |
+ |
TCF7L1 |
NNNTCATCTTCTACCACTCA |
NGG |
2 |
0.1753 |
Tier II |
| 5 |
NC_000019.10 |
19 |
5208984 |
+ |
PTPRS |
NNNTCATCTTCTACAACCCA |
NTG |
2 |
0.0367 |
Tier II |
| 6 |
NC_000002.12 |
2 |
149779041 |
- |
LINC01931 |
NNNTCATCCTCTTCGACCCA |
NGG |
2 |
0.2769 |
Tier III |
| 7 |
NC_000002.12 |
2 |
149779041 |
- |
MMADHC-DT |
NNNTCATCCTCTTCGACCCA |
NGG |
2 |
0.2769 |
Tier III |
| 8 |
NC_000009.12 |
9 |
38210789 |
- |
LOC107987064 |
NNNTCATTTTCTACCACCCA |
NGG |
2 |
0.2386 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
135210864 |
- |
Baz1b |
NNNTCATCTTCCACCACCCA |
NGG |
2 |
0.2584 |
Tier I |
| 2 |
NC_000077.6 |
11 |
46152214 |
- |
Nipal4 |
NNNTCATATTCTATGACCCA |
NGG |
2 |
0.4767 |
Tier II |
| 3 |
NC_000070.6 |
4 |
149949101 |
+ |
Spsb1 |
NNNTCATTTTCTAAGACCCA |
NGG |
2 |
0.3062 |
Tier II |
| 4 |
NC_000071.6 |
5 |
62657314 |
+ |
Arap2 |
NNNTCATCTTCTACAGCCCA |
NGG |
2 |
0.181 |
Tier II |
| 5 |
NC_000074.6 |
8 |
108580541 |
+ |
Zfhx3 |
NNNTCATCTTCTAGGACCCA |
NTG |
2 |
0.0 |
Tier II |
| 6 |
NC_000073.6 |
7 |
4827217 |
- |
Shisa7 |
NNNTCATCTTCTAGGACCCG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000085.6 |
19 |
24165054 |
+ |
Tjp2 |
NNNTCATTTTCTAGGACCCA |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000069.6 |
3 |
142076486 |
+ |
Bmpr1b |
NNNTCCTCTTCTACGCCCCA |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000071.6 |
5 |
117011917 |
+ |
Gm30028 |
NNNCCATCTTCTAGGACCCA |
NGG |
2 |
0.0 |
Tier III |
| 10 |
NC_000074.6 |
8 |
108580541 |
+ |
Lncbate1 |
NNNTCATCTTCTAGGACCCA |
NTG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
