Construct: sgRNA BRDN0001144931
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACAGCTATTGAACTTGCAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK24 (8428)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77058
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
98466521 |
- |
STK24 |
NNNGCTATTGAACTTGCAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
231040193 |
- |
C2orf72 |
NNNGCCAGTGAACTTGCAAG |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000005.10 |
5 |
59434648 |
- |
PDE4D |
NNNGCTATGGAACTTACAAG |
NGG |
2 |
0.619 |
Tier II |
| 4 |
NC_000003.12 |
3 |
143426927 |
+ |
SLC9A9 |
NNNGCTATAGAACTTGTAAG |
NGG |
2 |
0.4333 |
Tier II |
| 5 |
NC_000004.12 |
4 |
90621921 |
- |
CCSER1 |
NNNGGTATTGAACTTGCATG |
NGG |
2 |
0.3231 |
Tier II |
| 6 |
NC_000023.11 |
X |
10114950 |
+ |
WWC3 |
NNNGATATTGAACTTGAAAG |
NGG |
2 |
0.2667 |
Tier II |
| 7 |
NC_000008.11 |
8 |
144666146 |
+ |
ARHGAP39 |
NNNGATTTTGAACTTGCAAG |
NGG |
2 |
0.25 |
Tier II |
| 8 |
NC_000006.12 |
6 |
69224906 |
- |
ADGRB3 |
NNNTCTATTGAAATTGCAAG |
NGG |
2 |
0.1399 |
Tier II |
| 9 |
NC_000001.11 |
1 |
43736880 |
+ |
ST3GAL3 |
NNNGCTATTGAACTTGCACC |
NGG |
2 |
0.0887 |
Tier II |
| 10 |
NC_000005.10 |
5 |
145910111 |
+ |
GRXCR2 |
NNNGCTATTGTACCTGCAAG |
NGG |
2 |
0.0879 |
Tier II |
| 11 |
NC_000001.11 |
1 |
105977368 |
+ |
LINC01677 |
NNNGATATTGAACTTGCATG |
NGG |
2 |
0.3077 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
50886692 |
+ |
Stk26 |
NNNGCTATTGAACTTGCCAA |
NGG |
2 |
0.1786 |
Tier I |
| 2 |
NC_000085.6 |
19 |
45450168 |
+ |
Btrc |
NNNGGTCTTGAACTTGCAAG |
NGG |
2 |
0.2625 |
Tier II |
| 3 |
NC_000071.6 |
5 |
4806043 |
+ |
Cdk14 |
NNNGCTATTGAAATAGCAAG |
NGG |
2 |
0.2227 |
Tier II |
| 4 |
NC_000067.6 |
1 |
33612527 |
- |
Prim2 |
NNNGCTATAGAACTTGCAAG |
NGA |
2 |
0.0645 |
Tier II |
| 5 |
NC_000076.6 |
10 |
68254607 |
- |
Arid5b |
NNNGCTATAGAACTTGGAAG |
NGG |
2 |
0.0546 |
Tier II |
| 6 |
NC_000073.6 |
7 |
132285112 |
- |
Chst15 |
NNNGCTATTGTAGTTGCAAG |
NGG |
2 |
0.042 |
Tier II |
| 7 |
NC_000073.6 |
7 |
82951765 |
+ |
4933430H16Rik |
NNNGGTATTGAACTTGCATG |
NGG |
2 |
0.3231 |
Tier III |
| 8 |
NC_000082.6 |
16 |
24281636 |
- |
Gm38456 |
NNNGATATTGAACTTGCCAG |
NGG |
2 |
0.1088 |
Tier III |
| 9 |
NC_000073.6 |
7 |
59626429 |
+ |
Ipw |
NNNGCTATTGAACATGCAAG |
NGC |
2 |
0.0138 |
Tier III |
| 10 |
NC_000073.6 |
7 |
59626429 |
+ |
Snhg14 |
NNNGCTATTGAACATGCAAG |
NGC |
2 |
0.0138 |
Tier III |
Other clones with same target sequence:
(none)
