Construct: sgRNA BRDN0001144934
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAACAGCTATAAGAGCCGGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAST4 (375449)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76882
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
67160544 |
+ |
MAST4 |
NNNCAGCTATAAGAGCCGGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
169225801 |
+ |
NME7 |
NNNCAGCTATAAGAACTGGA |
NGG |
2 |
0.4392 |
Tier II |
| 3 |
NC_000017.11 |
17 |
280558 |
+ |
RPH3AL |
NNNCAGCTAGAAGAGCCGGG |
NGG |
2 |
0.3824 |
Tier II |
| 4 |
NC_000001.11 |
1 |
202823829 |
+ |
LOC124904583 |
NNNCAGTTATAACAGCCGGA |
NGG |
2 |
0.3421 |
Tier II |
| 5 |
NC_000007.14 |
7 |
14266153 |
- |
DGKB |
NNNCAGCTATAAGAGCTTGA |
NGG |
2 |
0.3111 |
Tier II |
| 6 |
NC_000002.12 |
2 |
204824016 |
+ |
PARD3B |
NNNCAGCTATAAGAGGAGGA |
NGG |
2 |
0.0718 |
Tier II |
| 7 |
NC_000015.10 |
15 |
93071061 |
+ |
RGMA |
NNNCAGCTTTAAGAGCGGGA |
NGG |
2 |
0.0353 |
Tier II |
| 8 |
NC_000001.11 |
1 |
202823829 |
+ |
MGAT4EP |
NNNCAGTTATAACAGCCGGA |
NGG |
2 |
0.3421 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
62322221 |
- |
Hk1 |
NNNCAGCGAGAAGAGCCGGA |
NGG |
2 |
0.3667 |
Tier II |
| 2 |
NC_000080.6 |
14 |
65299597 |
- |
Fbxo16 |
NNNCAGCTATAAGAACCGGA |
NAG |
2 |
0.244 |
Tier II |
| 3 |
NC_000086.7 |
X |
106044650 |
+ |
Atp7a |
NNNCAGCAATAAGAGCCGGA |
NAG |
2 |
0.2074 |
Tier II |
| 4 |
NC_000071.6 |
5 |
66136110 |
- |
Rbm47 |
NNNCAGCTATTAGAGCTGGA |
NGG |
2 |
0.1436 |
Tier II |
| 5 |
NC_000081.6 |
15 |
93628683 |
- |
Gm41385 |
NNNCAGCCCTAAGAGCCGGA |
NGG |
2 |
0.5714 |
Tier III |
| 6 |
NC_000073.6 |
7 |
143464248 |
- |
Gm33663 |
NNNCAGCTTTAAGAGCCAGA |
NGG |
2 |
0.4154 |
Tier III |
Other clones with same target sequence:
(none)
