Construct: sgRNA BRDN0001144935

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACAAATTGATAATATAGGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AKT3 (10000)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76218

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 243645937 - AKT3 NNNAATTGATAATATAGGAG NGG 0 1.0 Tier I
2 NC_000012.12 12 50354770 - FAM186A NNNAATTGATAACATAGGAA NGG 2 0.7401 Tier I
3 NC_000015.10 15 75619683 + SNUPN NNNAACTAATAATATAGGAG NGG 2 0.9091 Tier II
4 NC_000005.10 5 138303338 + CDC25C NNNAATTGGTAATATAGGAG NGG 1 0.6667 Tier II
5 NC_000011.10 11 125639918 - CHEK1 NNNAATTAATAATGTAGGAG NGG 2 0.4667 Tier II
6 NC_000018.10 18 36524995 + FHOD3 NNNAATTTATAATATAGTAG NGG 2 0.4167 Tier II
7 NC_000008.11 8 15123351 - SGCZ NNNAATTGATAATATAGTTG NGG 2 0.359 Tier II
8 NC_000020.11 20 33069671 - BPIFB3 NNNAATTGGTAATGTAGGAG NGG 2 0.3111 Tier II
9 NC_000004.12 4 92650111 + GRID2 NNNATTTCATAATATAGGAG NGG 2 0.2238 Tier II
10 NC_000006.12 6 25677454 + SCGN NNNAATTGATAATATGGGAG NGG 1 0.1923 Tier II
11 NC_000002.12 2 58204655 - FANCL NNNTATTGATAATATAGGAG NAG 2 0.165 Tier II
12 NC_000008.11 8 75528144 + HNF4G NNNAAGTGATAATATATGAG NGG 2 0.1429 Tier II
13 NC_000012.12 12 1863243 + CACNA2D4 NNNAATTGATAATATATGTG NGG 2 0.1346 Tier II
14 NC_000006.12 6 26640032 - ZNF322 NNNAATTGATAATGCAGGAG NGG 2 0.1273 Tier II
15 NC_000011.10 11 94457062 + MRE11 NNNAATTGATGATATGGGAG NGG 2 0.125 Tier II
16 NC_000008.11 8 120174142 - COL14A1 NNNATTTGATTATATAGGAG NGG 2 0.1119 Tier II
17 NC_000009.12 9 125926239 + PBX3 NNNAATTGATAATATAGGCC NGG 2 0.0887 Tier II
18 NC_000013.11 13 48432798 + RB1 NNNAATTTATAATATAGGAG NGA 2 0.0434 Tier II
19 NC_000013.11 13 48432798 + LPAR6 NNNAATTTATAATATAGGAG NGA 2 0.0434 Tier II
20 NC_000008.11 8 76999086 + PEX2 NNNAATTGATAATATAGGTG NGA 2 0.0374 Tier II
21 NC_000004.12 4 85913029 - ARHGAP24 NNNAATTGATAATGTAGGAG NGA 2 0.0324 Tier II
22 NC_000008.11 8 108728286 + TMEM74 NNNAATTGATAATGTAGGAG NGA 2 0.0324 Tier II
23 NC_000013.11 13 46234382 - LRRC63 NNNAATTGATAATACAGGAG NGC 2 0.0061 Tier II
24 NC_000016.10 16 83332051 + CDH13 NNNAATTGATAATATATGAG NGT 2 0.004 Tier II
25 NC_000001.11 1 108315457 + SLC25A24P1 NNNAAGTGATAATATAGGAA NGG 2 0.5357 Tier III
26 NC_000002.12 2 66018041 + LINC02934 NNNACTTGAAAATATAGGAG NGG 2 0.4286 Tier III
27 NC_000014.9 14 44043150 - LINC02307 NNNAATTGATAATATAGTTG NGG 2 0.359 Tier III
28 NC_000012.12 12 90256679 - LOC105369892 NNNCATTTATAATATAGGAG NGG 2 0.2206 Tier III
29 NC_000002.12 2 74753201 + LOC102724482 NNNAATTGATAACACAGGAG NGG 2 0.2153 Tier III
30 NC_000002.12 2 74753201 + LOC102724497 NNNAATTGATAACACAGGAG NGG 2 0.2153 Tier III
31 NC_000002.12 2 21259564 + LOC105374317 NNNAAGTGATAATACAGGAG NGG 2 0.1558 Tier III
32 NC_000005.10 5 8783349 - LOC101929307 NNNATTTGATAATATAGGAG NTG 2 0.0142 Tier III
33 NC_000005.10 5 91228194 - LOC107986432 NNNAATTGATAATAGAGGAG NTG 2 0.0019 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 177103006 - Akt3 NNNGATTGATAATATAGGAG NAG 2 0.162 Tier I
2 NC_000078.6 12 55099134 + Srp54a NNNAATTGATAATATAGTAA NGG 2 0.625 Tier II
3 NC_000078.6 12 55174194 + Srp54b NNNAATTGATAATATAGTAA NGG 2 0.625 Tier II
4 NC_000078.6 12 55249227 + Srp54c NNNAATTGATAATATAGTAA NGG 2 0.625 Tier II
5 NC_000070.6 4 87215775 + Slc24a2 NNNAATTGAAAATATAGTAG NGG 2 0.5714 Tier II
6 NC_000086.7 X 139643292 - Rnf128 NNNAATTGATATTATAGGAA NGG 2 0.3125 Tier II
7 NC_000072.6 6 24461190 + Iqub NNNAATTAATAATATATGAG NGG 2 0.25 Tier II
8 NC_000085.6 19 29261548 - Jak2 NNNAACTGATAATACAGGAG NGG 2 0.2479 Tier II
9 NC_000070.6 4 59672259 - E130308A19Rik NNNAATTAATAATCTAGGAG NGG 2 0.2143 Tier II
10 NC_000077.6 11 116436689 + Ubald2 NNNAATTGAGATTATAGGAG NGG 2 0.1667 Tier II
11 NC_000078.6 12 53543451 - Npas3 NNNAATTGATAATATAGGGC NGG 2 0.1607 Tier II
12 NC_000068.7 2 156291568 + Phf20 NNNAATTGATAATACAGGGG NGG 2 0.1023 Tier II
13 NC_000085.6 19 34676387 + Slc16a12 NNNAATTGATAATACAGGAG NAG 2 0.0707 Tier II
14 NC_000083.6 17 50885988 - Tbc1d5 NNNAATTGATAATATATGAG NGT 2 0.004 Tier II
15 NC_000080.6 14 74622654 + Gm41210 NNNAGTTGATAATATAGGGG NGG 2 0.27 Tier III
16 NC_000073.6 7 94446618 - Gm32647 NNNAATTGATAATATAGGAA NAG 2 0.2431 Tier III
17 NC_000081.6 15 13294965 - Gm46517 NNNAATTGTTATTATAGGAG NGG 2 0.2 Tier III
18 NC_000082.6 16 11982774 - Gm32278 NNNAATTGATAATTTATGAG NGG 2 0.1333 Tier III
19 NC_000067.6 1 161556713 - Gm31815 NNNATTTGATAATATAGGAG NAG 2 0.0943 Tier III
20 NC_000083.6 17 50885988 - Gm31083 NNNAATTGATAATATATGAG NGT 2 0.004 Tier III
21 NC_000069.6 3 64234594 - Gm34462 NNNAATTGATAATAACGGAG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)