Construct: sgRNA BRDN0001144935
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACAAATTGATAATATAGGAG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- AKT3 (10000)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000001.11 | 1 | 243645937 | - | AKT3 | NNNAATTGATAATATAGGAG | NGG | 0 | 1.0 | Tier I |
2 | NC_000012.12 | 12 | 50354770 | - | FAM186A | NNNAATTGATAACATAGGAA | NGG | 2 | 0.7401 | Tier I |
3 | NC_000015.10 | 15 | 75619683 | + | SNUPN | NNNAACTAATAATATAGGAG | NGG | 2 | 0.9091 | Tier II |
4 | NC_000005.10 | 5 | 138303338 | + | CDC25C | NNNAATTGGTAATATAGGAG | NGG | 1 | 0.6667 | Tier II |
5 | NC_000011.10 | 11 | 125639918 | - | CHEK1 | NNNAATTAATAATGTAGGAG | NGG | 2 | 0.4667 | Tier II |
6 | NC_000018.10 | 18 | 36524995 | + | FHOD3 | NNNAATTTATAATATAGTAG | NGG | 2 | 0.4167 | Tier II |
7 | NC_000008.11 | 8 | 15123351 | - | SGCZ | NNNAATTGATAATATAGTTG | NGG | 2 | 0.359 | Tier II |
8 | NC_000020.11 | 20 | 33069671 | - | BPIFB3 | NNNAATTGGTAATGTAGGAG | NGG | 2 | 0.3111 | Tier II |
9 | NC_000004.12 | 4 | 92650111 | + | GRID2 | NNNATTTCATAATATAGGAG | NGG | 2 | 0.2238 | Tier II |
10 | NC_000006.12 | 6 | 25677454 | + | SCGN | NNNAATTGATAATATGGGAG | NGG | 1 | 0.1923 | Tier II |
11 | NC_000002.12 | 2 | 58204655 | - | FANCL | NNNTATTGATAATATAGGAG | NAG | 2 | 0.165 | Tier II |
12 | NC_000008.11 | 8 | 75528144 | + | HNF4G | NNNAAGTGATAATATATGAG | NGG | 2 | 0.1429 | Tier II |
13 | NC_000012.12 | 12 | 1863243 | + | CACNA2D4 | NNNAATTGATAATATATGTG | NGG | 2 | 0.1346 | Tier II |
14 | NC_000006.12 | 6 | 26640032 | - | ZNF322 | NNNAATTGATAATGCAGGAG | NGG | 2 | 0.1273 | Tier II |
15 | NC_000011.10 | 11 | 94457062 | + | MRE11 | NNNAATTGATGATATGGGAG | NGG | 2 | 0.125 | Tier II |
16 | NC_000008.11 | 8 | 120174142 | - | COL14A1 | NNNATTTGATTATATAGGAG | NGG | 2 | 0.1119 | Tier II |
17 | NC_000009.12 | 9 | 125926239 | + | PBX3 | NNNAATTGATAATATAGGCC | NGG | 2 | 0.0887 | Tier II |
18 | NC_000013.11 | 13 | 48432798 | + | RB1 | NNNAATTTATAATATAGGAG | NGA | 2 | 0.0434 | Tier II |
19 | NC_000013.11 | 13 | 48432798 | + | LPAR6 | NNNAATTTATAATATAGGAG | NGA | 2 | 0.0434 | Tier II |
20 | NC_000008.11 | 8 | 76999086 | + | PEX2 | NNNAATTGATAATATAGGTG | NGA | 2 | 0.0374 | Tier II |
21 | NC_000004.12 | 4 | 85913029 | - | ARHGAP24 | NNNAATTGATAATGTAGGAG | NGA | 2 | 0.0324 | Tier II |
22 | NC_000008.11 | 8 | 108728286 | + | TMEM74 | NNNAATTGATAATGTAGGAG | NGA | 2 | 0.0324 | Tier II |
23 | NC_000013.11 | 13 | 46234382 | - | LRRC63 | NNNAATTGATAATACAGGAG | NGC | 2 | 0.0061 | Tier II |
24 | NC_000016.10 | 16 | 83332051 | + | CDH13 | NNNAATTGATAATATATGAG | NGT | 2 | 0.004 | Tier II |
25 | NC_000001.11 | 1 | 108315457 | + | SLC25A24P1 | NNNAAGTGATAATATAGGAA | NGG | 2 | 0.5357 | Tier III |
26 | NC_000002.12 | 2 | 66018041 | + | LINC02934 | NNNACTTGAAAATATAGGAG | NGG | 2 | 0.4286 | Tier III |
27 | NC_000014.9 | 14 | 44043150 | - | LINC02307 | NNNAATTGATAATATAGTTG | NGG | 2 | 0.359 | Tier III |
28 | NC_000012.12 | 12 | 90256679 | - | LOC105369892 | NNNCATTTATAATATAGGAG | NGG | 2 | 0.2206 | Tier III |
29 | NC_000002.12 | 2 | 74753201 | + | LOC102724482 | NNNAATTGATAACACAGGAG | NGG | 2 | 0.2153 | Tier III |
30 | NC_000002.12 | 2 | 74753201 | + | LOC102724497 | NNNAATTGATAACACAGGAG | NGG | 2 | 0.2153 | Tier III |
31 | NC_000002.12 | 2 | 21259564 | + | LOC105374317 | NNNAAGTGATAATACAGGAG | NGG | 2 | 0.1558 | Tier III |
32 | NC_000005.10 | 5 | 8783349 | - | LOC101929307 | NNNATTTGATAATATAGGAG | NTG | 2 | 0.0142 | Tier III |
33 | NC_000005.10 | 5 | 91228194 | - | LOC107986432 | NNNAATTGATAATAGAGGAG | NTG | 2 | 0.0019 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000067.6 | 1 | 177103006 | - | Akt3 | NNNGATTGATAATATAGGAG | NAG | 2 | 0.162 | Tier I |
2 | NC_000078.6 | 12 | 55099134 | + | Srp54a | NNNAATTGATAATATAGTAA | NGG | 2 | 0.625 | Tier II |
3 | NC_000078.6 | 12 | 55174194 | + | Srp54b | NNNAATTGATAATATAGTAA | NGG | 2 | 0.625 | Tier II |
4 | NC_000078.6 | 12 | 55249227 | + | Srp54c | NNNAATTGATAATATAGTAA | NGG | 2 | 0.625 | Tier II |
5 | NC_000070.6 | 4 | 87215775 | + | Slc24a2 | NNNAATTGAAAATATAGTAG | NGG | 2 | 0.5714 | Tier II |
6 | NC_000086.7 | X | 139643292 | - | Rnf128 | NNNAATTGATATTATAGGAA | NGG | 2 | 0.3125 | Tier II |
7 | NC_000072.6 | 6 | 24461190 | + | Iqub | NNNAATTAATAATATATGAG | NGG | 2 | 0.25 | Tier II |
8 | NC_000085.6 | 19 | 29261548 | - | Jak2 | NNNAACTGATAATACAGGAG | NGG | 2 | 0.2479 | Tier II |
9 | NC_000070.6 | 4 | 59672259 | - | E130308A19Rik | NNNAATTAATAATCTAGGAG | NGG | 2 | 0.2143 | Tier II |
10 | NC_000077.6 | 11 | 116436689 | + | Ubald2 | NNNAATTGAGATTATAGGAG | NGG | 2 | 0.1667 | Tier II |
11 | NC_000078.6 | 12 | 53543451 | - | Npas3 | NNNAATTGATAATATAGGGC | NGG | 2 | 0.1607 | Tier II |
12 | NC_000068.7 | 2 | 156291568 | + | Phf20 | NNNAATTGATAATACAGGGG | NGG | 2 | 0.1023 | Tier II |
13 | NC_000085.6 | 19 | 34676387 | + | Slc16a12 | NNNAATTGATAATACAGGAG | NAG | 2 | 0.0707 | Tier II |
14 | NC_000083.6 | 17 | 50885988 | - | Tbc1d5 | NNNAATTGATAATATATGAG | NGT | 2 | 0.004 | Tier II |
15 | NC_000080.6 | 14 | 74622654 | + | Gm41210 | NNNAGTTGATAATATAGGGG | NGG | 2 | 0.27 | Tier III |
16 | NC_000073.6 | 7 | 94446618 | - | Gm32647 | NNNAATTGATAATATAGGAA | NAG | 2 | 0.2431 | Tier III |
17 | NC_000081.6 | 15 | 13294965 | - | Gm46517 | NNNAATTGTTATTATAGGAG | NGG | 2 | 0.2 | Tier III |
18 | NC_000082.6 | 16 | 11982774 | - | Gm32278 | NNNAATTGATAATTTATGAG | NGG | 2 | 0.1333 | Tier III |
19 | NC_000067.6 | 1 | 161556713 | - | Gm31815 | NNNATTTGATAATATAGGAG | NAG | 2 | 0.0943 | Tier III |
20 | NC_000083.6 | 17 | 50885988 | - | Gm31083 | NNNAATTGATAATATATGAG | NGT | 2 | 0.004 | Tier III |
21 | NC_000069.6 | 3 | 64234594 | - | Gm34462 | NNNAATTGATAATAACGGAG | NGG | 2 | 0.0 | Tier III |