Construct: sgRNA BRDN0001144936
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCAACACAGATCATACCTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NEK3 (4752)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77358
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
52144692 |
+ |
NEK3 |
NNNACACAGATCATACCTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
57253892 |
+ |
ZNF805 |
NNNACACAGATCATACCTCA |
NGT |
2 |
0.0151 |
Tier I |
| 3 |
NC_000023.11 |
X |
104751819 |
- |
IL1RAPL2 |
NNNACACAGATCATAACTCC |
NGG |
2 |
0.4286 |
Tier II |
| 4 |
NC_000021.9 |
21 |
37507750 |
- |
DYRK1A |
NNNACACAGGTCATGCCTCG |
NGG |
2 |
0.3611 |
Tier II |
| 5 |
NC_000009.12 |
9 |
83524513 |
- |
FRMD3 |
NNNACACAGATCACACCTCT |
NGG |
2 |
0.2 |
Tier II |
| 6 |
NC_000011.10 |
11 |
40323761 |
+ |
LRRC4C |
NNNACACAGATCCTACCTCA |
NGG |
2 |
0.1974 |
Tier II |
| 7 |
NC_000009.12 |
9 |
137227547 |
+ |
RNF224 |
NNNACACTGATCATACCTCC |
NGG |
2 |
0.1837 |
Tier II |
| 8 |
NC_000003.12 |
3 |
60362497 |
- |
FHIT |
NNNACACAGCTCATACCTAG |
NGG |
2 |
0.1429 |
Tier II |
| 9 |
NC_000009.12 |
9 |
89348458 |
- |
SECISBP2 |
NNNACACAGATCAAACCTGG |
NGG |
2 |
0.0774 |
Tier II |
| 10 |
NC_000002.12 |
2 |
191354856 |
+ |
MYO1B |
NNNACACAGATCATACCTCT |
NGA |
2 |
0.0486 |
Tier II |
| 11 |
NC_000015.10 |
15 |
100734582 |
+ |
LOC105371024 |
NNNACCCAGATCATACCTAG |
NGG |
2 |
0.1948 |
Tier III |
| 12 |
NC_000002.12 |
2 |
241984652 |
- |
LINC01237 |
NNNACACAGATCATACCGTG |
NGG |
2 |
0.1538 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
82389330 |
- |
Cyp2d11 |
NNNACACAGATCATGCCTGG |
NGG |
2 |
0.0813 |
Tier I |
| 2 |
NC_000070.6 |
4 |
104990901 |
+ |
Fyb2 |
NNNACACAGATAATACCACG |
NGG |
2 |
0.4762 |
Tier II |
| 3 |
NC_000080.6 |
14 |
18810559 |
+ |
Ube2e2 |
NNNACACAGATCAAACCTCT |
NGG |
2 |
0.4333 |
Tier II |
| 4 |
NC_000084.6 |
18 |
16631241 |
+ |
Cdh2 |
NNNACACAGATCATAACTCC |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000084.6 |
18 |
24631791 |
+ |
Elp2 |
NNNACACAGATCATTCCTCG |
NAG |
2 |
0.0519 |
Tier II |
Other clones with same target sequence:
(none)
