Construct: sgRNA BRDN0001144937
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGACACATGTGGATTGACAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- VRK3 (51231)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75549
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
50016051 |
+ |
VRK3 |
NNNCACATGTGGATTGACAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
62211299 |
+ |
RTKN2 |
NNNCAAATTTGGATTGACAA |
NGG |
2 |
0.4952 |
Tier II |
| 3 |
NC_000001.11 |
1 |
210673019 |
+ |
HHAT |
NNNCACAATTGGATTGACAA |
NGG |
2 |
0.4267 |
Tier II |
| 4 |
NC_000006.12 |
6 |
168033152 |
+ |
KIF25 |
NNNCACATGTGGATTGACTG |
NGG |
2 |
0.4118 |
Tier II |
| 5 |
NC_000011.10 |
11 |
25010058 |
- |
LUZP2 |
NNNCACATGGGGATTGACAA |
NGA |
2 |
0.0347 |
Tier II |
| 6 |
NC_000011.10 |
11 |
126379812 |
+ |
ST3GAL4 |
NNNCACATGTGGATTGACAA |
NTC |
2 |
0.0 |
Tier II |
| 7 |
NC_000018.10 |
18 |
24372518 |
+ |
OSBPL1A |
NNNCACATGTGTATTCACAA |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000006.12 |
6 |
75918130 |
+ |
MYO6 |
NNNCACCTGTGGATTTACAA |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000001.11 |
1 |
214074261 |
- |
LINC02775 |
NNNTTCATGTGGATTGACAA |
NGG |
2 |
0.2909 |
Tier III |
| 10 |
NC_000006.12 |
6 |
44782083 |
- |
LOC101929770 |
NNNCATATGTGGACTGACAA |
NGG |
2 |
0.2653 |
Tier III |
| 11 |
NC_000001.11 |
1 |
212633277 |
- |
LINC02773 |
NNNTACATGTGGTTTGACAA |
NGG |
2 |
0.24 |
Tier III |
| 12 |
NC_000002.12 |
2 |
45211827 |
- |
LINC01121 |
NNNCACATGTGGACTGATAA |
NGG |
2 |
0.1837 |
Tier III |
| 13 |
NC_000018.10 |
18 |
24372518 |
+ |
LOC124904268 |
NNNCACATGTGTATTCACAA |
NGG |
2 |
0.0 |
Tier III |
| 14 |
NC_000008.11 |
8 |
24363664 |
- |
ADAM7-AS1 |
NNNCACATGTTGATTTACAA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
166285103 |
+ |
Ildr2 |
NNNCATGTGTGGATTGACAA |
NGG |
2 |
0.6555 |
Tier II |
| 2 |
NC_000083.6 |
17 |
69216611 |
+ |
Epb41l3 |
NNNTACAAGTGGATTGACAA |
NGG |
2 |
0.64 |
Tier II |
| 3 |
NC_000079.6 |
13 |
68728725 |
+ |
Adcy2 |
NNNCACAAATGGATTGACAA |
NGG |
2 |
0.5143 |
Tier II |
| 4 |
NC_000068.7 |
2 |
41503983 |
+ |
Lrp1b |
NNNTACATTTGGATTGACAA |
NGG |
2 |
0.4267 |
Tier II |
| 5 |
NC_000084.6 |
18 |
75061838 |
+ |
Dym |
NNNCATCTGTGGATTGACAA |
NGG |
2 |
0.4062 |
Tier II |
| 6 |
NC_000070.6 |
4 |
3704542 |
- |
Lyn |
NNNCACATCTGGAATGACAA |
NGG |
2 |
0.3333 |
Tier II |
| 7 |
NC_000086.7 |
X |
56660002 |
- |
Slc9a6 |
NNNCACATGTGGATAGAAAA |
NGG |
2 |
0.3117 |
Tier II |
| 8 |
NC_000084.6 |
18 |
34584103 |
+ |
Brd8dc |
NNNCAAATGTGGACTGACAA |
NGG |
2 |
0.2653 |
Tier II |
| 9 |
NC_000078.6 |
12 |
84325279 |
- |
Zfp410 |
NNNCACAGGTGGATTGACAC |
NGG |
2 |
0.1667 |
Tier II |
| 10 |
NC_000079.6 |
13 |
49353056 |
+ |
Bicd2 |
NNNCCCATGTGGATTGACAC |
NGG |
2 |
0.1136 |
Tier II |
| 11 |
NC_000075.6 |
9 |
10275465 |
- |
Cntn5 |
NNNCACATGTGTATTGACAC |
NGG |
2 |
0.0874 |
Tier II |
| 12 |
NC_000071.6 |
5 |
30396651 |
+ |
Otof |
NNNCACATGTGGATAGAGAA |
NGG |
2 |
0.0772 |
Tier II |
| 13 |
NC_000071.6 |
5 |
69288546 |
+ |
Kctd8 |
NNNCACATGTGGATAGACAA |
NTG |
2 |
0.0226 |
Tier II |
| 14 |
NC_000081.6 |
15 |
83334366 |
+ |
Arfgap3 |
NNNCACATGTGAATTGACAA |
NGC |
2 |
0.0207 |
Tier II |
| 15 |
NC_000069.6 |
3 |
66169571 |
- |
Veph1 |
NNNCACCTGTGGATTGACAA |
NGT |
2 |
0.0071 |
Tier II |
| 16 |
NC_000082.6 |
16 |
13475678 |
+ |
Gm32817 |
NNNCACATGTGGATTGTGAA |
NGG |
2 |
0.0178 |
Tier III |
Other clones with same target sequence:
(none)
