Construct: sgRNA BRDN0001144938
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TAATGTACACGACTCCATGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- KDR (3791)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77757
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
55115372 |
+ |
KDR |
NNNTGTACACGACTCCATGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
133134950 |
- |
NCKAP5 |
NNNAGTAAACGACTCCATGT |
NGG |
2 |
0.3095 |
Tier II |
| 3 |
NC_000016.10 |
16 |
49711247 |
- |
ZNF423 |
NNNTGTACACGTCTCCCTGT |
NGG |
2 |
0.0588 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
60620356 |
+ |
Itgb6 |
NNNTGTACACGAGTCCCTGT |
NGG |
2 |
0.0241 |
Tier I |
| 2 |
NC_000074.6 |
8 |
48342838 |
- |
Tenm3 |
NNNTATACACGAATCCATGT |
NGG |
2 |
0.3333 |
Tier II |
| 3 |
NC_000072.6 |
6 |
100581137 |
- |
Shq1 |
NNNTGTAAACTACTCCATGT |
NGG |
2 |
0.25 |
Tier II |
| 4 |
NC_000084.6 |
18 |
60961692 |
- |
Camk2a |
NNNTGTACAGGACTCCATGA |
NGG |
2 |
0.2188 |
Tier II |
| 5 |
NC_000079.6 |
13 |
114997290 |
- |
Itga1 |
NNNTGTACATGAGTCCATGT |
NGG |
2 |
0.1283 |
Tier II |
| 6 |
NC_000080.6 |
14 |
117057602 |
- |
Gpc6 |
NNNTGTACACAACTTCATGT |
NGG |
2 |
0.0667 |
Tier II |
| 7 |
NC_000074.6 |
8 |
12790142 |
- |
Atp11a |
NNNTGTACACGCCTCCATGC |
NGG |
2 |
0.0239 |
Tier II |
| 8 |
NC_000074.6 |
8 |
93353584 |
- |
Ces1h |
NNNTGTACACGACTCCATGT |
NTT |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
