Construct: sgRNA BRDN0001144940
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGGATGTACCGAGTCAGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIP4K2C (79837)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77684
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
57599126 |
+ |
PIP4K2C |
NNNGATGTACCGAGTCAGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
142361337 |
+ |
TSNARE1 |
NNNGATGTGCCGGGTCAGTG |
NGG |
2 |
0.4348 |
Tier II |
| 3 |
NC_000003.12 |
3 |
175446651 |
- |
NAALADL2 |
NNNCATGTACCCAGTCAGTG |
NGG |
2 |
0.2803 |
Tier II |
| 4 |
NC_000007.14 |
7 |
148021842 |
+ |
CNTNAP2 |
NNNGCTGTACCGAGTCAGTG |
NCG |
2 |
0.0536 |
Tier II |
| 5 |
NC_000020.11 |
20 |
53611811 |
- |
LOC105372672 |
NNNGATGTACCGAGCCAGTG |
NAG |
2 |
0.0707 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
98135400 |
- |
Notch2 |
NNNGAGGTCCCGAGTCAGTG |
NGG |
2 |
0.3265 |
Tier I |
| 2 |
NC_000076.6 |
10 |
127201125 |
- |
Pip4k2c |
NNNCATGTACCGAGTCAGTG |
NAG |
2 |
0.1373 |
Tier I |
| 3 |
NC_000080.6 |
14 |
33541541 |
- |
Frmpd2 |
NNNGATGTACCAGGTCAGTG |
NGG |
2 |
0.6087 |
Tier II |
| 4 |
NC_000075.6 |
9 |
43235407 |
+ |
Oaf |
NNNGATGTCCCGATTCAGTG |
NGG |
2 |
0.1524 |
Tier II |
| 5 |
NC_000076.6 |
10 |
127201125 |
- |
F420014N23Rik |
NNNCATGTACCGAGTCAGTG |
NAG |
2 |
0.1373 |
Tier III |
Other clones with same target sequence:
(none)
