Construct: sgRNA BRDN0001144943

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATCACACATCATGTAAAGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS6KA3 (6197)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75942

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 20209361 - RPS6KA3 NNNACACATCATGTAAAGGA NGG 0 1.0 Tier I
2 NC_000023.11 X 84156148 - RPS6KA6 NNNACTCATCATGTTAAGGA NGG 2 0.1429 Tier I
3 NC_000009.12 9 108899594 - ELP1 NNNACTCATCATATAAAGGA NGG 2 0.6593 Tier II
4 NC_000008.11 8 6532715 + ANGPT2 NNNTCACATCATGTAAAGGA NGG 1 0.6364 Tier II
5 NC_000008.11 8 6532715 + MCPH1 NNNTCACATCATGTAAAGGA NGG 1 0.6364 Tier II
6 NC_000009.12 9 116935660 - ASTN2 NNNACACAGCAAGTAAAGGA NGG 2 0.4952 Tier II
7 NC_000006.12 6 170395417 - FAM120B NNNACACAGCATGTAAAGGG NGG 2 0.4734 Tier II
8 NC_000001.11 1 248513229 - OR2G6 NNNACACATCATGTAAATTA NGG 2 0.4444 Tier II
9 NC_000013.11 13 26651422 + WASF3 NNNATACATCATGTAAAAGA NGG 2 0.4406 Tier II
10 NC_000014.9 14 52602462 - GPR137C NNNACAGAACATGTAAAGGA NGG 2 0.437 Tier II
11 NC_000001.11 1 34143034 - CSMD2 NNNACACAGCAGGTAAAGGA NGG 2 0.3095 Tier II
12 NC_000018.10 18 11844091 - GNAL NNNACACTTCATGTAAAGTA NGG 2 0.2857 Tier II
13 NC_000011.10 11 88568021 + GRM5 NNNACACATATTGTAAAGGA NGG 2 0.2667 Tier II
14 NC_000004.12 4 8496278 - TRMT44 NNNACACATCATACAAAGGA NGG 2 0.2637 Tier II
15 NC_000011.10 11 82864843 - PRCP NNNAAACATCATTTAAAGGA NGG 2 0.1714 Tier II
16 NC_000009.12 9 135829957 + CAMSAP1 NNNGCACATCATGTAGAGGA NGG 2 0.1202 Tier II
17 NC_000010.11 10 13503432 + BEND7 NNNACACATCCTTTAAAGGA NGG 2 0.12 Tier II
18 NC_000004.12 4 184022557 + STOX2 NNNACACATCATGCCAAGGA NGG 2 0.0779 Tier II
19 NC_000018.10 18 59593344 - CCBE1 NNNACACATCATCTAATGGA NGG 2 0.0561 Tier II
20 NC_000002.12 2 73217934 - SMYD5 NNNATACATCATGTAAAGGA NGA 2 0.0442 Tier II
21 NC_000006.12 6 2207109 + GMDS NNNACACATCATGTAAAAGA NGT 2 0.0112 Tier II
22 NC_000008.11 8 124740720 + LOC105375741 NNNACACATTGTGTAAAGGA NGG 2 0.6118 Tier III
23 NC_000013.11 13 84082449 - LOC105370287 NNNAAATATCATGTAAAGGA NGG 2 0.4643 Tier III
24 NC_000001.11 1 248513229 - LOC105373277 NNNACACATCATGTAAATTA NGG 2 0.4444 Tier III
25 NC_000001.11 1 248534834 + LOC105373277 NNNACACATCATGTAAATTA NGG 2 0.4444 Tier III
26 NC_000014.9 14 22426317 + TRA NNNACATCTCATGTAAAGGA NGG 2 0.3482 Tier III
27 NC_000014.9 14 22426317 + TRD NNNACATCTCATGTAAAGGA NGG 2 0.3482 Tier III
28 NC_000014.9 14 22426317 + TRD-AS1 NNNACATCTCATGTAAAGGA NGG 2 0.3482 Tier III
29 NC_000004.12 4 79862809 - PCAT4 NNNACACATCATGCAAGGGA NGG 2 0.0504 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 159311508 + Rps6ka3 NNNACACATCATGTGAAGGA NGG 1 0.65 Tier I
2 NC_000072.6 6 90589430 + Aldh1l1 NNNACACATCATGAAAAGGT NGG 2 0.3714 Tier I
3 NC_000084.6 18 38962274 + Arhgap26 NNNACACAATATGTAAAGGA NGG 2 0.8739 Tier II
4 NC_000084.6 18 38962274 + Gm5820 NNNACACAATATGTAAAGGA NGG 2 0.8739 Tier II
5 NC_000068.7 2 66094790 + Galnt3 NNNACACACTATGTAAAGGA NGG 2 0.8688 Tier II
6 NC_000070.6 4 48354106 - Invs NNNACACAGCATGTAAAGGG NGG 2 0.4734 Tier II
7 NC_000073.6 7 72160304 + Mctp2 NNNACACATCATGTAAATTA NGG 2 0.4444 Tier II
8 NC_000076.6 10 127465564 + R3hdm2 NNNACACATAAGGTAAAGGA NGG 2 0.4333 Tier II
9 NC_000073.6 7 130174046 - Fgfr2 NNNGCACATCATGTAAAAGA NGG 2 0.4327 Tier II
10 NC_000070.6 4 36627883 + Lingo2 NNNAAACAGCATGTAAAGGA NGG 2 0.3537 Tier II
11 NC_000070.6 4 49126965 + Plppr1 NNNACATATCATTTAAAGGA NGG 2 0.2438 Tier II
12 NC_000074.6 8 37584721 + Sgcz NNNACATATCATTTAAAGGA NGG 2 0.2438 Tier II
13 NC_000075.6 9 21572956 + Carm1 NNNACTCATCATGTCAAGGA NGG 2 0.1948 Tier II
14 NC_000071.6 5 130442004 - Caln1 NNNAAACATCTTGTAAAGGA NGG 2 0.1758 Tier II
15 NC_000077.6 11 100617517 - Dnajc7 NNNCCACATCATGTAAAGCA NGG 2 0.1582 Tier II
16 NC_000067.6 1 100084674 + Cntnap5b NNNACACATGATTTAAAGGA NGG 2 0.1167 Tier II
17 NC_000070.6 4 68802894 - Brinp1 NNNACACATCATGAAATGGA NGG 2 0.0825 Tier II
18 NC_000078.6 12 34351431 - Hdac9 NNNACACATCAAGTAAAGGA NGA 2 0.0556 Tier II
19 NC_000070.6 4 82333182 + Nfib NNNACACATCATGTAAAGGG NGA 2 0.0531 Tier II
20 NC_000080.6 14 59494650 - Cab39l NNNACACATCTTGTAATGGA NGG 2 0.041 Tier II
21 NC_000069.6 3 26229461 + Nlgn1 NNNACACATCATGTTAAGGA NGA 2 0.0139 Tier II
22 NC_000075.6 9 80342145 - Impg1 NNNACACATCATGAAAAGGA NGC 2 0.0138 Tier II
23 NC_000081.6 15 72836805 + Trappc9 NNNACACATCATCTAAAGGA NGC 2 0.0094 Tier II
24 NC_000071.6 5 44685859 + Ldb2 NNNACACATCATGGAAAGAA NGG 2 0.0 Tier II
25 NC_000067.6 1 127336977 + Mgat5 NNNACACATCATGGAGAGGA NGG 2 0.0 Tier II
26 NC_000069.6 3 145526804 + Col24a1 NNNACACATCATTGAAAGGA NGG 2 0.0 Tier II
27 NC_000067.6 1 92724141 + Gm29483 NNNACACACCATGTAAGGGA NGG 2 0.1629 Tier III
28 NC_000068.7 2 6263770 + A230108P19Rik NNNACACAGCATGTAAGGGA NGG 2 0.1092 Tier III
29 NC_000078.6 12 115070125 - Igh NNNACACATCATCTAAAGGA NAG 2 0.1092 Tier III
30 NC_000078.6 12 115296629 - Igh NNNACACATCATCTAAAGGA NTG 2 0.0164 Tier III
31 NC_000078.6 12 115818243 - Igh NNNACACATCATCTAAAGGA NTG 2 0.0164 Tier III
32 NC_000081.6 15 72836805 + Gm41343 NNNACACATCATCTAAAGGA NGC 2 0.0094 Tier III
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Other clones with same target sequence:

(none)