Construct: sgRNA BRDN0001144950
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTTTGGGAGAAATCAAAAG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- BMPR2 (659)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000002.12 | 2 | 202464953 | + | BMPR2 | NNNTTGGGAGAAATCAAAAG | NGG | 0 | 1.0 | Tier I |
2 | NC_000008.11 | 8 | 73250271 | - | C8orf89 | NNNTTGGGAGAAATCAAAAG | NTA | 2 | 0.0 | Tier I |
3 | NC_000001.11 | 1 | 207616019 | + | CR1 | NNNTTGGAAGAAATCAAAAA | NGG | 2 | 0.9375 | Tier II |
4 | NC_000001.11 | 1 | 14729867 | + | KAZN | NNNTTGGAAAAAATCAAAAG | NGG | 2 | 0.9333 | Tier II |
5 | NC_000007.14 | 7 | 157961191 | - | PTPRN2 | NNNTTGGAAAAAATCAAAAG | NGG | 2 | 0.9333 | Tier II |
6 | NC_000015.10 | 15 | 71640864 | - | THSD4 | NNNTTTAGAGAAATCAAAAG | NGG | 2 | 0.6667 | Tier II |
7 | NC_000012.12 | 12 | 113058827 | - | DTX1 | NNNTTGGGAAAAATCAAATG | NGG | 2 | 0.5026 | Tier II |
8 | NC_000003.12 | 3 | 33367947 | - | FBXL2 | NNNATGGAAGAAATCAAAAG | NGG | 2 | 0.4762 | Tier II |
9 | NC_000001.11 | 1 | 243445685 | - | SDCCAG8 | NNNTTTGGAGAAATCAAAAT | NGG | 2 | 0.4667 | Tier II |
10 | NC_000001.11 | 1 | 22836170 | - | EPHB2 | NNNTTTGGGGAAATCAAAAG | NGG | 2 | 0.4444 | Tier II |
11 | NC_000002.12 | 2 | 45929584 | - | PRKCE | NNNTTGGAAGAAATCAAGAG | NGG | 2 | 0.4 | Tier II |
12 | NC_000012.12 | 12 | 23984063 | - | SOX5 | NNNTTGAGAGAAATCAAAGG | NGG | 2 | 0.375 | Tier II |
13 | NC_000011.10 | 11 | 5407544 | - | OR51B5 | NNNGTGTGAGAAATCAAAAG | NGG | 2 | 0.3571 | Tier II |
14 | NC_000018.10 | 18 | 32433792 | + | GAREM1 | NNNTTGAGAGAAATCAAAAG | NAG | 2 | 0.2593 | Tier II |
15 | NC_000002.12 | 2 | 236242503 | + | ASB18 | NNNTTGGGAAAAATCAAAAG | NAG | 2 | 0.242 | Tier II |
16 | NC_000010.11 | 10 | 112681511 | + | VTI1A | NNNTTGGGAAAAATCAAAAG | NAG | 2 | 0.242 | Tier II |
17 | NC_000005.10 | 5 | 42556965 | - | GHR | NNNTTGGGAGAACTCAAAAA | NGG | 2 | 0.1974 | Tier II |
18 | NC_000013.11 | 13 | 108538942 | - | MYO16 | NNNTTGGGAGAACTCAAAAA | NGG | 2 | 0.1974 | Tier II |
19 | NC_000006.12 | 6 | 109760804 | - | FIG4 | NNNTTGGGATAAATAAAAAG | NGG | 2 | 0.1806 | Tier II |
20 | NC_000003.12 | 3 | 60427813 | - | FHIT | NNNTTGGGAGATATCAAATG | NGG | 2 | 0.1795 | Tier II |
21 | NC_000003.12 | 3 | 87934638 | - | HTR1F | NNNTTGGGAGAAATCAACAA | NGG | 2 | 0.1786 | Tier II |
22 | NC_000008.11 | 8 | 118299103 | + | SAMD12 | NNNGTGGGAGACATCAAAAG | NGG | 2 | 0.1645 | Tier II |
23 | NC_000006.12 | 6 | 123396221 | - | TRDN | NNNTTGGCAGAAATCAAAAG | NAG | 2 | 0.1595 | Tier II |
24 | NC_000004.12 | 4 | 110046420 | - | ELOVL6 | NNNTTGGGAGAGCTCAAAAG | NGG | 2 | 0.152 | Tier II |
25 | NC_000005.10 | 5 | 145885362 | - | GRXCR2 | NNNTTGGGAGAAAAAAAAAG | NGG | 2 | 0.1376 | Tier II |
26 | NC_000011.10 | 11 | 31161253 | + | DCDC1 | NNNCTGGGAGAAATCAAACG | NGG | 2 | 0.1339 | Tier II |
27 | NC_000002.12 | 2 | 210439993 | - | LANCL1 | NNNTTGGGAGAGATCAGAAG | NGG | 2 | 0.1275 | Tier II |
28 | NC_000009.12 | 9 | 34561194 | - | CNTFR | NNNTGGGGAGAAATCACAAG | NGG | 2 | 0.1129 | Tier II |
29 | NC_000004.12 | 4 | 153196801 | - | TRIM2 | NNNTTGGGAGAGATCATAAG | NGG | 2 | 0.0963 | Tier II |
30 | NC_000023.11 | X | 77917562 | + | ATP7A | NNNTTGGGAGAAATAAAAGG | NGG | 2 | 0.0833 | Tier II |
31 | NC_000003.12 | 3 | 123160018 | - | PDIA5 | NNNTTGGAAGAAATTAAAAG | NGG | 2 | 0.0667 | Tier II |
32 | NC_000004.12 | 4 | 56055404 | + | CRACD | NNNTTGGGAGAAATAAAAAG | NAG | 2 | 0.0576 | Tier II |
33 | NC_000023.11 | X | 15572777 | - | ACE2 | NNNTTGGGAGAAATAAAAAG | NAG | 2 | 0.0576 | Tier II |
34 | NC_000011.10 | 11 | 58950741 | - | GLYATL1 | NNNTTGGGATAAATTAAAAG | NGG | 2 | 0.0542 | Tier II |
35 | NC_000005.10 | 5 | 107989990 | - | FBXL17 | NNNTTGAGAGAAATGAAAAG | NGG | 2 | 0.05 | Tier II |
36 | NC_000006.12 | 6 | 162200529 | - | PRKN | NNNTTGGGAAAAATCAAAAG | NTG | 2 | 0.0364 | Tier II |
37 | NC_000023.11 | X | 150638878 | + | MTM1 | NNNTTGCGAGAAATCAAAAG | NTG | 2 | 0.0268 | Tier II |
38 | NC_000007.14 | 7 | 37296466 | - | ELMO1 | NNNTTGGGAGTAATCAAAAG | NGA | 2 | 0.0214 | Tier II |
39 | NC_000003.12 | 3 | 159372737 | + | SCHIP1 | NNNTTGGGAAAAATCAAAAG | NGT | 2 | 0.0151 | Tier II |
40 | NC_000003.12 | 3 | 159372737 | + | IQCJ-SCHIP1 | NNNTTGGGAAAAATCAAAAG | NGT | 2 | 0.0151 | Tier II |
41 | NC_000004.12 | 4 | 16217022 | - | TAPT1 | NNNTTGGGAGAAATGAAAAG | NAG | 2 | 0.013 | Tier II |
42 | NC_000012.12 | 12 | 86599807 | + | MGAT4C | NNNTTGGGAGAAATAAAAAG | NGT | 2 | 0.0036 | Tier II |
43 | NC_000008.11 | 8 | 67735858 | + | CPA6 | NNNTTGGGAGAAATCAGAAG | NGT | 2 | 0.0028 | Tier II |
44 | NC_000004.12 | 4 | 171947211 | - | GALNTL6 | NNNGTGGGAGAAAGCAAAAG | NGG | 2 | 0.0 | Tier II |
45 | NC_000004.12 | 4 | 1842596 | - | LETM1 | NNNTTGGGAGAAAGCAAATG | NGG | 2 | 0.0 | Tier II |
46 | NC_000007.14 | 7 | 5755497 | - | RNF216 | NNNTTGGGAGAAAGCACAAG | NGG | 2 | 0.0 | Tier II |
47 | NC_000011.10 | 11 | 96142315 | + | MAML2 | NNNTTGGGAGAAATCTGAAG | NGG | 2 | 0.0 | Tier II |
48 | NC_000003.12 | 3 | 181258459 | + | SOX2-OT | NNNTTGGGAGAGATCAAAAA | NGG | 2 | 0.6771 | Tier III |
49 | NC_000005.10 | 5 | 132411351 | + | CARINH | NNNTTGGTAAAAATCAAAAG | NGG | 2 | 0.5833 | Tier III |
50 | NC_000003.12 | 3 | 178975031 | + | LOC124906307 | NNNTTGGGAAAAAACAAAAG | NGG | 2 | 0.5778 | Tier III |
51 | NC_000013.11 | 13 | 78072837 | - | OBI1-AS1 | NNNTAGAGAGAAATCAAAAG | NGG | 2 | 0.5 | Tier III |
52 | NC_000004.12 | 4 | 26080074 | + | LINC02357 | NNNTTGGAAGAAATCAAAAG | NAG | 2 | 0.2593 | Tier III |
53 | NC_000002.12 | 2 | 33650978 | - | LOC105374455 | NNNAAGGGAGAAATCAAAAG | NGG | 2 | 0.2381 | Tier III |
54 | NC_000020.11 | 20 | 60209395 | + | MIR646HG | NNNTTGAGAGAAATCAACAG | NGG | 2 | 0.1905 | Tier III |
55 | NC_000008.11 | 8 | 118299103 | + | LOC105375724 | NNNGTGGGAGACATCAAAAG | NGG | 2 | 0.1645 | Tier III |
56 | NC_000002.12 | 2 | 210439993 | - | LANCL1-AS1 | NNNTTGGGAGAGATCAGAAG | NGG | 2 | 0.1275 | Tier III |
57 | NC_000006.12 | 6 | 97707312 | + | LOC101927314 | NNNTTGGGAGAAGTCATAAG | NGG | 2 | 0.087 | Tier III |
58 | NC_000011.10 | 11 | 58950741 | - | LOC283194 | NNNTTGGGATAAATTAAAAG | NGG | 2 | 0.0542 | Tier III |
59 | NC_000017.11 | 17 | 55619904 | - | LOC101927389 | NNNTTGGGAGAAATCAAATG | NGC | 2 | 0.012 | Tier III |
60 | NC_000002.12 | 2 | 75266211 | - | LOC105374811 | NNNTTGGGAGAAAGCAAAAG | NAG | 2 | 0.0 | Tier III |
61 | NC_000002.12 | 2 | 75266211 | - | LOC107985900 | NNNTTGGGAGAAAGCAAAAG | NAG | 2 | 0.0 | Tier III |
62 | NC_000008.11 | 8 | 73250271 | - | LOC105375901 | NNNTTGGGAGAAATCAAAAG | NTA | 2 | 0.0 | Tier III |
63 | NC_000014.9 | 14 | 21184710 | - | LOC100129923 | NNNTTGGGAGAAATCAAAAG | NAT | 2 | 0.0 | Tier III |
64 | NC_000021.9 | 21 | 16178639 | + | MIR99AHG | NNNTTGGTAGAAAGCAAAAG | NGG | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000067.6 | 1 | 59813732 | + | Bmpr2 | NNNGTGGGAGAAATCAAAAG | NGG | 1 | 0.625 | Tier I |
2 | NC_000085.6 | 19 | 14513214 | + | Tle4 | NNNTTAAGAGAAATCAAAAG | NGG | 2 | 1.0 | Tier II |
3 | NC_000085.6 | 19 | 22434206 | + | Trpm3 | NNNTTGAGAGAAGTCAAAAG | NGG | 2 | 0.6522 | Tier II |
4 | NC_000077.6 | 11 | 118312803 | + | Timp2 | NNNTGGGGAGAAATCAAAAG | NGG | 1 | 0.64 | Tier II |
5 | NC_000077.6 | 11 | 66402461 | + | Shisa6 | NNNGTGGGAGAAGTCAAAAG | NGG | 2 | 0.4076 | Tier II |
6 | NC_000069.6 | 3 | 24210017 | + | Naaladl2 | NNNTTGGGAGAAATCAAGAG | NGG | 1 | 0.4 | Tier II |
7 | NC_000084.6 | 18 | 62946617 | - | Apcdd1 | NNNTGGGCAGAAATCAAAAG | NGG | 2 | 0.3938 | Tier II |
8 | NC_000072.6 | 6 | 53409674 | - | Creb5 | NNNTTGTGGGAAATCAAAAG | NGG | 2 | 0.381 | Tier II |
9 | NC_000073.6 | 7 | 105681567 | - | Dnhd1 | NNNTTGAGAGATATCAAAAG | NGG | 2 | 0.3333 | Tier II |
10 | NC_000070.6 | 4 | 32463936 | + | Bach2 | NNNATCGGAGAAATCAAAAG | NGG | 2 | 0.3247 | Tier II |
11 | NC_000077.6 | 11 | 89567432 | + | Ankfn1 | NNNTAGGGAGAAAACAAAAG | NGG | 2 | 0.3095 | Tier II |
12 | NC_000071.6 | 5 | 20570076 | + | Magi2 | NNNTTGGGAGAAAACAAAAC | NGG | 2 | 0.2653 | Tier II |
13 | NC_000070.6 | 4 | 132905994 | + | Fam76a | NNNTTGGGTGAAATCAAAAC | NGG | 2 | 0.2571 | Tier II |
14 | NC_000067.6 | 1 | 162448520 | + | Dnm3 | NNNTGGGGAGAAATCAAAGG | NGG | 2 | 0.24 | Tier II |
15 | NC_000073.6 | 7 | 24535400 | - | Irgq | NNNTTGGGAGAAATCAGAAG | NGG | 1 | 0.1765 | Tier II |
16 | NC_000073.6 | 7 | 139628510 | + | Cfap46 | NNNTTGGGAGAGCTCAAAAG | NGG | 2 | 0.152 | Tier II |
17 | NC_000076.6 | 10 | 60829952 | + | Unc5b | NNNTGGGGAGAAATAAAAAG | NGG | 2 | 0.1422 | Tier II |
18 | NC_000068.7 | 2 | 106133722 | - | Dcdc5 | NNNCTGGGAGAAATCAAACG | NGG | 2 | 0.1339 | Tier II |
19 | NC_000079.6 | 13 | 89062068 | + | Edil3 | NNNTTTGGAGAAATCACAAG | NGG | 2 | 0.1176 | Tier II |
20 | NC_000076.6 | 10 | 123185208 | + | Usp15 | NNNTTGGGAGAAATGAAAAA | NGG | 2 | 0.0469 | Tier II |
21 | NC_000078.6 | 12 | 83408292 | + | Dpf3 | NNNTTGGGAGAAATAGAAAG | NGG | 2 | 0.0427 | Tier II |
22 | NC_000068.7 | 2 | 72242219 | - | Rapgef4 | NNNTTGGGAGAAAACAAAAG | NGT | 2 | 0.01 | Tier II |
23 | NC_000083.6 | 17 | 34160222 | - | H2-DMb1 | NNNTTGGGAGAAATCAAAAG | NCC | 2 | 0.0 | Tier II |
24 | NC_000080.6 | 14 | 109494131 | - | Gm30216 | NNNTTGGGAGAAAACAAAAA | NGG | 2 | 0.5804 | Tier III |
25 | NC_000073.6 | 7 | 139628510 | + | Gm33031 | NNNTTGGGAGAGCTCAAAAG | NGG | 2 | 0.152 | Tier III |
26 | NC_000069.6 | 3 | 120516672 | - | 6530403H02Rik | NNNTTGGGGGAAATAAAAAG | NGG | 2 | 0.1481 | Tier III |
27 | NC_000076.6 | 10 | 123185208 | + | Gm4129 | NNNTTGGGAGAAATGAAAAA | NGG | 2 | 0.0469 | Tier III |
28 | NC_000079.6 | 13 | 105412468 | - | Gm31130 | NNNTAGGGAGAAATTAAAAG | NGG | 2 | 0.0333 | Tier III |
29 | NC_000077.6 | 11 | 25978303 | + | 5730522E02Rik | NNNTTGGGAGAAATCAAAAG | NAT | 2 | 0.0 | Tier III |