Construct: sgRNA BRDN0001144950

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCTTTGGGAGAAATCAAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BMPR2 (659)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76390

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 202464953 + BMPR2 NNNTTGGGAGAAATCAAAAG NGG 0 1.0 Tier I
2 NC_000008.11 8 73250271 - C8orf89 NNNTTGGGAGAAATCAAAAG NTA 2 0.0 Tier I
3 NC_000001.11 1 207616019 + CR1 NNNTTGGAAGAAATCAAAAA NGG 2 0.9375 Tier II
4 NC_000001.11 1 14729867 + KAZN NNNTTGGAAAAAATCAAAAG NGG 2 0.9333 Tier II
5 NC_000007.14 7 157961191 - PTPRN2 NNNTTGGAAAAAATCAAAAG NGG 2 0.9333 Tier II
6 NC_000015.10 15 71640864 - THSD4 NNNTTTAGAGAAATCAAAAG NGG 2 0.6667 Tier II
7 NC_000012.12 12 113058827 - DTX1 NNNTTGGGAAAAATCAAATG NGG 2 0.5026 Tier II
8 NC_000003.12 3 33367947 - FBXL2 NNNATGGAAGAAATCAAAAG NGG 2 0.4762 Tier II
9 NC_000001.11 1 243445685 - SDCCAG8 NNNTTTGGAGAAATCAAAAT NGG 2 0.4667 Tier II
10 NC_000001.11 1 22836170 - EPHB2 NNNTTTGGGGAAATCAAAAG NGG 2 0.4444 Tier II
11 NC_000002.12 2 45929584 - PRKCE NNNTTGGAAGAAATCAAGAG NGG 2 0.4 Tier II
12 NC_000012.12 12 23984063 - SOX5 NNNTTGAGAGAAATCAAAGG NGG 2 0.375 Tier II
13 NC_000011.10 11 5407544 - OR51B5 NNNGTGTGAGAAATCAAAAG NGG 2 0.3571 Tier II
14 NC_000018.10 18 32433792 + GAREM1 NNNTTGAGAGAAATCAAAAG NAG 2 0.2593 Tier II
15 NC_000002.12 2 236242503 + ASB18 NNNTTGGGAAAAATCAAAAG NAG 2 0.242 Tier II
16 NC_000010.11 10 112681511 + VTI1A NNNTTGGGAAAAATCAAAAG NAG 2 0.242 Tier II
17 NC_000005.10 5 42556965 - GHR NNNTTGGGAGAACTCAAAAA NGG 2 0.1974 Tier II
18 NC_000013.11 13 108538942 - MYO16 NNNTTGGGAGAACTCAAAAA NGG 2 0.1974 Tier II
19 NC_000006.12 6 109760804 - FIG4 NNNTTGGGATAAATAAAAAG NGG 2 0.1806 Tier II
20 NC_000003.12 3 60427813 - FHIT NNNTTGGGAGATATCAAATG NGG 2 0.1795 Tier II
21 NC_000003.12 3 87934638 - HTR1F NNNTTGGGAGAAATCAACAA NGG 2 0.1786 Tier II
22 NC_000008.11 8 118299103 + SAMD12 NNNGTGGGAGACATCAAAAG NGG 2 0.1645 Tier II
23 NC_000006.12 6 123396221 - TRDN NNNTTGGCAGAAATCAAAAG NAG 2 0.1595 Tier II
24 NC_000004.12 4 110046420 - ELOVL6 NNNTTGGGAGAGCTCAAAAG NGG 2 0.152 Tier II
25 NC_000005.10 5 145885362 - GRXCR2 NNNTTGGGAGAAAAAAAAAG NGG 2 0.1376 Tier II
26 NC_000011.10 11 31161253 + DCDC1 NNNCTGGGAGAAATCAAACG NGG 2 0.1339 Tier II
27 NC_000002.12 2 210439993 - LANCL1 NNNTTGGGAGAGATCAGAAG NGG 2 0.1275 Tier II
28 NC_000009.12 9 34561194 - CNTFR NNNTGGGGAGAAATCACAAG NGG 2 0.1129 Tier II
29 NC_000004.12 4 153196801 - TRIM2 NNNTTGGGAGAGATCATAAG NGG 2 0.0963 Tier II
30 NC_000023.11 X 77917562 + ATP7A NNNTTGGGAGAAATAAAAGG NGG 2 0.0833 Tier II
31 NC_000003.12 3 123160018 - PDIA5 NNNTTGGAAGAAATTAAAAG NGG 2 0.0667 Tier II
32 NC_000004.12 4 56055404 + CRACD NNNTTGGGAGAAATAAAAAG NAG 2 0.0576 Tier II
33 NC_000023.11 X 15572777 - ACE2 NNNTTGGGAGAAATAAAAAG NAG 2 0.0576 Tier II
34 NC_000011.10 11 58950741 - GLYATL1 NNNTTGGGATAAATTAAAAG NGG 2 0.0542 Tier II
35 NC_000005.10 5 107989990 - FBXL17 NNNTTGAGAGAAATGAAAAG NGG 2 0.05 Tier II
36 NC_000006.12 6 162200529 - PRKN NNNTTGGGAAAAATCAAAAG NTG 2 0.0364 Tier II
37 NC_000023.11 X 150638878 + MTM1 NNNTTGCGAGAAATCAAAAG NTG 2 0.0268 Tier II
38 NC_000007.14 7 37296466 - ELMO1 NNNTTGGGAGTAATCAAAAG NGA 2 0.0214 Tier II
39 NC_000003.12 3 159372737 + SCHIP1 NNNTTGGGAAAAATCAAAAG NGT 2 0.0151 Tier II
40 NC_000003.12 3 159372737 + IQCJ-SCHIP1 NNNTTGGGAAAAATCAAAAG NGT 2 0.0151 Tier II
41 NC_000004.12 4 16217022 - TAPT1 NNNTTGGGAGAAATGAAAAG NAG 2 0.013 Tier II
42 NC_000012.12 12 86599807 + MGAT4C NNNTTGGGAGAAATAAAAAG NGT 2 0.0036 Tier II
43 NC_000008.11 8 67735858 + CPA6 NNNTTGGGAGAAATCAGAAG NGT 2 0.0028 Tier II
44 NC_000004.12 4 171947211 - GALNTL6 NNNGTGGGAGAAAGCAAAAG NGG 2 0.0 Tier II
45 NC_000004.12 4 1842596 - LETM1 NNNTTGGGAGAAAGCAAATG NGG 2 0.0 Tier II
46 NC_000007.14 7 5755497 - RNF216 NNNTTGGGAGAAAGCACAAG NGG 2 0.0 Tier II
47 NC_000011.10 11 96142315 + MAML2 NNNTTGGGAGAAATCTGAAG NGG 2 0.0 Tier II
48 NC_000003.12 3 181258459 + SOX2-OT NNNTTGGGAGAGATCAAAAA NGG 2 0.6771 Tier III
49 NC_000005.10 5 132411351 + CARINH NNNTTGGTAAAAATCAAAAG NGG 2 0.5833 Tier III
50 NC_000003.12 3 178975031 + LOC124906307 NNNTTGGGAAAAAACAAAAG NGG 2 0.5778 Tier III
51 NC_000013.11 13 78072837 - OBI1-AS1 NNNTAGAGAGAAATCAAAAG NGG 2 0.5 Tier III
52 NC_000004.12 4 26080074 + LINC02357 NNNTTGGAAGAAATCAAAAG NAG 2 0.2593 Tier III
53 NC_000002.12 2 33650978 - LOC105374455 NNNAAGGGAGAAATCAAAAG NGG 2 0.2381 Tier III
54 NC_000020.11 20 60209395 + MIR646HG NNNTTGAGAGAAATCAACAG NGG 2 0.1905 Tier III
55 NC_000008.11 8 118299103 + LOC105375724 NNNGTGGGAGACATCAAAAG NGG 2 0.1645 Tier III
56 NC_000002.12 2 210439993 - LANCL1-AS1 NNNTTGGGAGAGATCAGAAG NGG 2 0.1275 Tier III
57 NC_000006.12 6 97707312 + LOC101927314 NNNTTGGGAGAAGTCATAAG NGG 2 0.087 Tier III
58 NC_000011.10 11 58950741 - LOC283194 NNNTTGGGATAAATTAAAAG NGG 2 0.0542 Tier III
59 NC_000017.11 17 55619904 - LOC101927389 NNNTTGGGAGAAATCAAATG NGC 2 0.012 Tier III
60 NC_000002.12 2 75266211 - LOC105374811 NNNTTGGGAGAAAGCAAAAG NAG 2 0.0 Tier III
61 NC_000002.12 2 75266211 - LOC107985900 NNNTTGGGAGAAAGCAAAAG NAG 2 0.0 Tier III
62 NC_000008.11 8 73250271 - LOC105375901 NNNTTGGGAGAAATCAAAAG NTA 2 0.0 Tier III
63 NC_000014.9 14 21184710 - LOC100129923 NNNTTGGGAGAAATCAAAAG NAT 2 0.0 Tier III
64 NC_000021.9 21 16178639 + MIR99AHG NNNTTGGTAGAAAGCAAAAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 59813732 + Bmpr2 NNNGTGGGAGAAATCAAAAG NGG 1 0.625 Tier I
2 NC_000085.6 19 14513214 + Tle4 NNNTTAAGAGAAATCAAAAG NGG 2 1.0 Tier II
3 NC_000085.6 19 22434206 + Trpm3 NNNTTGAGAGAAGTCAAAAG NGG 2 0.6522 Tier II
4 NC_000077.6 11 118312803 + Timp2 NNNTGGGGAGAAATCAAAAG NGG 1 0.64 Tier II
5 NC_000077.6 11 66402461 + Shisa6 NNNGTGGGAGAAGTCAAAAG NGG 2 0.4076 Tier II
6 NC_000069.6 3 24210017 + Naaladl2 NNNTTGGGAGAAATCAAGAG NGG 1 0.4 Tier II
7 NC_000084.6 18 62946617 - Apcdd1 NNNTGGGCAGAAATCAAAAG NGG 2 0.3938 Tier II
8 NC_000072.6 6 53409674 - Creb5 NNNTTGTGGGAAATCAAAAG NGG 2 0.381 Tier II
9 NC_000073.6 7 105681567 - Dnhd1 NNNTTGAGAGATATCAAAAG NGG 2 0.3333 Tier II
10 NC_000070.6 4 32463936 + Bach2 NNNATCGGAGAAATCAAAAG NGG 2 0.3247 Tier II
11 NC_000077.6 11 89567432 + Ankfn1 NNNTAGGGAGAAAACAAAAG NGG 2 0.3095 Tier II
12 NC_000071.6 5 20570076 + Magi2 NNNTTGGGAGAAAACAAAAC NGG 2 0.2653 Tier II
13 NC_000070.6 4 132905994 + Fam76a NNNTTGGGTGAAATCAAAAC NGG 2 0.2571 Tier II
14 NC_000067.6 1 162448520 + Dnm3 NNNTGGGGAGAAATCAAAGG NGG 2 0.24 Tier II
15 NC_000073.6 7 24535400 - Irgq NNNTTGGGAGAAATCAGAAG NGG 1 0.1765 Tier II
16 NC_000073.6 7 139628510 + Cfap46 NNNTTGGGAGAGCTCAAAAG NGG 2 0.152 Tier II
17 NC_000076.6 10 60829952 + Unc5b NNNTGGGGAGAAATAAAAAG NGG 2 0.1422 Tier II
18 NC_000068.7 2 106133722 - Dcdc5 NNNCTGGGAGAAATCAAACG NGG 2 0.1339 Tier II
19 NC_000079.6 13 89062068 + Edil3 NNNTTTGGAGAAATCACAAG NGG 2 0.1176 Tier II
20 NC_000076.6 10 123185208 + Usp15 NNNTTGGGAGAAATGAAAAA NGG 2 0.0469 Tier II
21 NC_000078.6 12 83408292 + Dpf3 NNNTTGGGAGAAATAGAAAG NGG 2 0.0427 Tier II
22 NC_000068.7 2 72242219 - Rapgef4 NNNTTGGGAGAAAACAAAAG NGT 2 0.01 Tier II
23 NC_000083.6 17 34160222 - H2-DMb1 NNNTTGGGAGAAATCAAAAG NCC 2 0.0 Tier II
24 NC_000080.6 14 109494131 - Gm30216 NNNTTGGGAGAAAACAAAAA NGG 2 0.5804 Tier III
25 NC_000073.6 7 139628510 + Gm33031 NNNTTGGGAGAGCTCAAAAG NGG 2 0.152 Tier III
26 NC_000069.6 3 120516672 - 6530403H02Rik NNNTTGGGGGAAATAAAAAG NGG 2 0.1481 Tier III
27 NC_000076.6 10 123185208 + Gm4129 NNNTTGGGAGAAATGAAAAA NGG 2 0.0469 Tier III
28 NC_000079.6 13 105412468 - Gm31130 NNNTAGGGAGAAATTAAAAG NGG 2 0.0333 Tier III
29 NC_000077.6 11 25978303 + 5730522E02Rik NNNTTGGGAGAAATCAAAAG NAT 2 0.0 Tier III
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Other clones with same target sequence:

(none)