Construct: sgRNA BRDN0001144952

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCGGGCTTCAGGAATAAAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CMPK2 (129607)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75804

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 6863569 + CMPK2 NNNGGCTTCAGGAATAAAGG NGG 0 1.0 Tier I
2 NC_000012.12 12 121921583 + CFAP251 NNNGGCTTCAGGAATACAGG NAG 2 0.0458 Tier I
3 NC_000006.12 6 75876289 - MYO6 NNNGGCTTATGGAATAAAGG NGG 2 0.7563 Tier II
4 NC_000005.10 5 11001440 - CTNND2 NNNGGCTTCAGAAATAAATG NGG 2 0.6222 Tier II
5 NC_000017.11 17 1508239 + INPP5K NNNGGCTTCAGAAAAAAAGG NGG 2 0.5404 Tier II
6 NC_000004.12 4 15709388 + BST1 NNNGGCTAGAGGAATAAAGG NGG 2 0.4952 Tier II
7 NC_000005.10 5 81049232 - RASGRF2 NNNGGCTTGGGGAATAAAGG NGG 2 0.3439 Tier II
8 NC_000001.11 1 78634505 + IFI44L NNNTGCTTCAGAAATAAAGG NGG 2 0.3394 Tier II
9 NC_000012.12 12 75295539 + CAPS2 NNNGGCTTCAGGAATAAGAG NGG 2 0.2857 Tier II
10 NC_000003.12 3 41743386 + ULK4 NNNATCTTCAGGAATAAAGG NGG 2 0.27 Tier II
11 NC_000001.11 1 176840320 + PAPPA2 NNNGGCTTCAGGAATAAAGG NAG 1 0.2593 Tier II
12 NC_000001.11 1 49910042 + AGBL4 NNNGGCTTAAGGTATAAAGG NGG 2 0.2571 Tier II
13 NC_000017.11 17 50600587 + CACNA1G NNNGGCTTTAGGAATAAAGG NAG 2 0.2269 Tier II
14 NC_000017.11 17 48395447 - SKAP1 NNNGGCTTGCGGAATAAAGG NGG 2 0.2063 Tier II
15 NC_000008.11 8 103376317 + CTHRC1 NNNGGCTTCAGACATAAAGG NGG 2 0.1965 Tier II
16 NC_000019.10 19 1647990 - TCF3 NNNGGCTTCAGGAATGAAGG NGG 1 0.1923 Tier II
17 NC_000010.11 10 37797260 + ZNF248 NNNGGCTTCAGGAATGAAGT NGG 2 0.1346 Tier II
18 NC_000002.12 2 40409326 + SLC8A1 NNNGGCTTCAGGAATATAGT NGG 2 0.0933 Tier II
19 NC_000010.11 10 17001169 + CUBN NNNGGCTTCAGGAGTGAAGG NGG 2 0.0897 Tier II
20 NC_000012.12 12 62290858 - USP15 NNNAGCTTCAGGAATAAAGG NGA 2 0.0625 Tier II
21 NC_000008.11 8 66791553 - SGK3 NNNGGCATCAGGAATAAAGG NGA 2 0.0608 Tier II
22 NC_000008.11 8 66791553 - C8orf44-SGK3 NNNGGCATCAGGAATAAAGG NGA 2 0.0608 Tier II
23 NC_000001.11 1 56957342 - C8B NNNGCCTTCAGGAATAAAGG NGA 2 0.0546 Tier II
24 NC_000004.12 4 93542893 - GRID2 NNNGGCTTCAGGAAGAAAGA NGG 2 0.0469 Tier II
25 NC_000004.12 4 182241914 - TENM3 NNNGGCTTCAGGAATAAAGA NGT 2 0.0151 Tier II
26 NC_000003.12 3 159323813 + SCHIP1 NNNGGCTTCAGGTATAAAGG NTG 2 0.0117 Tier II
27 NC_000003.12 3 159323813 + IQCJ-SCHIP1 NNNGGCTTCAGGTATAAAGG NTG 2 0.0117 Tier II
28 NC_000009.12 9 128570232 + SPTAN1 NNNGGCTTCAGTAATTAAGG NGG 2 0.0 Tier II
29 NC_000006.12 6 23411385 - LOC105374976 NNNGGCTTCTTGAATAAAGG NGG 2 0.3394 Tier III
30 NC_000010.11 10 106184812 + LINC02624 NNNGGCTACAGGAATAAGGG NGG 2 0.32 Tier III
31 NC_000012.12 12 75295539 + CAPS2-AS1 NNNGGCTTCAGGAATAAGAG NGG 2 0.2857 Tier III
32 NC_000011.10 11 121900209 - LOC107984402 NNNTGCTTCAGGAATAAATG NGG 2 0.2424 Tier III
33 NC_000011.10 11 87439817 + LOC107984361 NNNGGCTTCAGGAATAGGGG NGG 2 0.0706 Tier III
34 NC_000013.11 13 84445765 + LINC00333 NNNGGCTTCAGGTATATAGG NGG 2 0.04 Tier III
35 NC_000013.11 13 84445765 + LOC105370289 NNNGGCTTCAGGTATATAGG NGG 2 0.04 Tier III
36 NC_000006.12 6 156044274 - LOC101928923 NNNGGCTTCAGGAAGAAAGG NGA 2 0.0035 Tier III
37 NC_000008.11 8 56639917 + LOC105375851 NNNGGCTTCAGGAATATAGG NGT 2 0.0022 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 134476404 + Taf7l NNNGGCTTCAGGAATAACGG NGG 1 0.1905 Tier I
2 NC_000078.6 12 26471416 - Cmpk2 NNNGGCTTCTGGAATAAAGG NTG 2 0.0344 Tier I
3 NC_000084.6 18 57722801 + Ccdc192 NNNAGCTTTAGGAATAAAGG NGG 2 0.7875 Tier II
4 NC_000067.6 1 82580308 + Col4a4 NNNGGCTTCAGGGATAAAGA NGG 2 0.6114 Tier II
5 NC_000076.6 10 86046474 + Fbxo7 NNNGACTTCAGGAATAAATG NGG 2 0.5778 Tier II
6 NC_000067.6 1 106618500 + Bcl2 NNNGGCTTCAGGGACAAAGG NGG 2 0.1779 Tier II
7 NC_000073.6 7 35741466 + Dpy19l3 NNNGGCTTTAGGAATAGAGG NGG 2 0.1544 Tier II
8 NC_000069.6 3 97164195 - Acp6 NNNGGCTACAGGAATAGAGG NGG 2 0.1412 Tier II
9 NC_000079.6 13 104344304 + Adamts6 NNNGGCTTCAGGAACAAAGC NGG 2 0.1169 Tier II
10 NC_000069.6 3 30323154 - Mecom NNNGGCTTCAGGAAAAGAGG NGG 2 0.1022 Tier II
11 NC_000071.6 5 139146568 - Prkar1b NNNTGCTTCAGGAATGAAGG NGG 2 0.0699 Tier II
12 NC_000071.6 5 150589477 + N4bp2l1 NNNGGCTGCAGGAATAAAGG NGA 2 0.0509 Tier II
13 NC_000079.6 13 101690186 - Pik3r1 NNNGGCTTCGGGAATAAAGG NGT 2 0.009 Tier II
14 NC_000071.6 5 125696118 + Tmem132b NNNGGCTTCAGGAAGAAAGG NGA 2 0.0035 Tier II
15 NC_000067.6 1 125705220 + Gpr39 NNNGGCATCAGGAATCAAGG NGG 2 0.0 Tier II
16 NC_000073.6 7 89408627 - Fzd4 NNNGGCTTCAGGAATCTAGG NGG 2 0.0 Tier II
17 NC_000069.6 3 67504397 + Rarres1 NNNTGCTTCAGGAATCAAGG NGG 2 0.0 Tier II
18 NC_000067.6 1 73147537 + Gm31197 NNNGGTTTCAGGAATAAAGG NGA 2 0.0645 Tier III
19 NC_000067.6 1 73147537 + Gm29185 NNNGGTTTCAGGAATAAAGG NGA 2 0.0645 Tier III
20 NC_000073.6 7 129677164 - Gm33248 NNNGGCTTCAGGGAGAAAGG NGG 2 0.0326 Tier III
21 NC_000080.6 14 52991279 + Tcra NNNGGCTTCAGGAACAAAGG NGA 2 0.0189 Tier III
22 NC_000080.6 14 52991279 + Trav4d-4 NNNGGCTTCAGGAACAAAGG NGA 2 0.0189 Tier III
23 NC_000080.6 14 53302449 + Tcra NNNGGCTTCAGGAACAAAGG NGA 2 0.0189 Tier III
24 NC_000080.6 14 53302449 + Trav4n-4 NNNGGCTTCAGGAACAAAGG NGA 2 0.0189 Tier III
25 NC_000080.6 14 53684067 + Tcra NNNGGCTTCAGGAACAAAGG NGA 2 0.0189 Tier III
26 NC_000080.6 14 53684067 + Trav4-4-dv10 NNNGGCTTCAGGAACAAAGG NGA 2 0.0189 Tier III
27 NC_000068.7 2 160273310 + Gm46773 NNNGGCTTCAGGTATAAAGG NGC 2 0.0067 Tier III
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Other clones with same target sequence:

(none)