Construct: sgRNA BRDN0001144954
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATGTCCTCGAAAAGTACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NME9 (347736)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76837
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
138318185 |
- |
NME9 |
NNNGTCCTCGAAAAGTACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
59859901 |
+ |
PDE4D |
NNNGTCCTCTAAAAGTACAA |
NGG |
2 |
0.7617 |
Tier II |
| 3 |
NC_000003.12 |
3 |
49735039 |
- |
IP6K1 |
NNNGTCCTCCAAAGGTACAG |
NGG |
2 |
0.1867 |
Tier II |
| 4 |
NC_000019.10 |
19 |
45165755 |
+ |
TRAPPC6A |
NNNGTGCTCGAAAAGGACAG |
NGG |
2 |
0.1731 |
Tier II |
| 5 |
NC_000013.11 |
13 |
113001191 |
+ |
MCF2L |
NNNGTCCTGGAAAAGTAGAG |
NGG |
2 |
0.0825 |
Tier II |
| 6 |
NC_000017.11 |
17 |
81659351 |
- |
PDE6G |
NNNGTCCTCCAAAAGTACAG |
NGA |
2 |
0.0278 |
Tier II |
| 7 |
NC_000005.10 |
5 |
59859901 |
+ |
LOC107986350 |
NNNGTCCTCTAAAAGTACAA |
NGG |
2 |
0.7617 |
Tier III |
| 8 |
NC_000020.11 |
20 |
6733814 |
+ |
LINC01713 |
NNNGTCCTGGAAAAGTACAG |
NTG |
2 |
0.0241 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
5138030 |
- |
Emid1 |
NNNGTCCTCTAAAAGAACAG |
NGG |
2 |
0.7386 |
Tier II |
| 2 |
NC_000072.6 |
6 |
38824612 |
- |
Hipk2 |
NNNGTCCTTGAAGAGTACAG |
NGG |
2 |
0.5707 |
Tier II |
| 3 |
NC_000070.6 |
4 |
103635959 |
+ |
Dab1 |
NNNGTACTCCAAAAGTACAG |
NGG |
2 |
0.3714 |
Tier II |
| 4 |
NC_000078.6 |
12 |
73961207 |
+ |
S100a11-ps |
NNNGTCCTCCAAAAGTACAG |
NGG |
1 |
0.4 |
Tier III |
Other clones with same target sequence:
(none)
