Construct: sgRNA BRDN0001144958
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATAAGGAGTTACCGTATCCC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BTK (695)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77071
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
101357514 |
+ |
BTK |
NNNAGGAGTTACCGTATCCC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
31180211 |
- |
DMD |
NNNAGGATTTACCCTATCCC |
NGG |
2 |
0.2679 |
Tier II |
| 3 |
NC_000018.10 |
18 |
5193444 |
- |
AKAIN1 |
NNNAGAAGTTACCTTATCCC |
NGG |
2 |
0.2667 |
Tier II |
| 4 |
NC_000007.14 |
7 |
44617952 |
- |
OGDH |
NNNAGGAGGTACCCTATCCC |
NGG |
2 |
0.2653 |
Tier II |
| 5 |
NC_000012.12 |
12 |
12719110 |
- |
CDKN1B |
NNNAGGAATTACCGTATCCC |
NAG |
2 |
0.2593 |
Tier II |
| 6 |
NC_000002.12 |
2 |
53806988 |
- |
ERLEC1 |
NNNAGGAGTTACAGTAACCC |
NGG |
2 |
0.2051 |
Tier II |
| 7 |
NC_000002.12 |
2 |
53806988 |
- |
GPR75-ASB3 |
NNNAGGAGTTACAGTAACCC |
NGG |
2 |
0.2051 |
Tier II |
| 8 |
NC_000013.11 |
13 |
28415788 |
- |
FLT1 |
NNNAGGAGTTACAGTATCCA |
NGG |
2 |
0.1923 |
Tier II |
| 9 |
NC_000015.10 |
15 |
47191592 |
+ |
SEMA6D |
NNNAGGACTTACCGTGTCCC |
NGG |
2 |
0.1183 |
Tier II |
| 10 |
NC_000006.12 |
6 |
67889042 |
+ |
LOC105377845 |
NNNAGGAGTTCCCCTATCCC |
NGG |
2 |
0.1714 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
132622027 |
+ |
Lhpp |
NNNATGGGTTACCGTATCCC |
NGG |
2 |
0.2118 |
Tier II |
| 2 |
NC_000070.6 |
4 |
78021903 |
+ |
Ptprd |
NNNAGGAGTCACAGTATCCC |
NGG |
2 |
0.2051 |
Tier II |
| 3 |
NC_000086.7 |
X |
25282823 |
- |
Gm5169 |
NNNAGGAGTTCCCGTGTCCC |
NGG |
2 |
0.0769 |
Tier II |
| 4 |
NC_000086.7 |
X |
26685119 |
- |
Gm14525 |
NNNAGGAGTTCCCGTGTCCC |
NGG |
2 |
0.0769 |
Tier II |
| 5 |
NC_000086.7 |
X |
26918087 |
- |
Gm6121 |
NNNAGGAGTTCCCGTGTCCC |
NGG |
2 |
0.0769 |
Tier II |
| 6 |
NC_000070.6 |
4 |
105844935 |
+ |
Gm29922 |
NNNAAGAGTTACCGGATCCC |
NGG |
2 |
0.0433 |
Tier III |
Other clones with same target sequence:
(none)
