Construct: sgRNA BRDN0001144967
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TAAAAGGGCGATCTAATGAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TGFBR1 (7046)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76646
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
99132660 |
- |
TGFBR1 |
NNNAAGGGCGATCTAATGAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
76773128 |
+ |
ROBO2 |
NNNAAGTGCAATCTAATGAA |
NGG |
2 |
0.5333 |
Tier II |
| 3 |
NC_000010.11 |
10 |
4394085 |
+ |
LINC00703 |
NNNGAGGGCGATCTAGTGAA |
NGG |
2 |
0.1202 |
Tier III |
| 4 |
NC_000010.11 |
10 |
4394085 |
+ |
LOC105376372 |
NNNGAGGGCGATCTAGTGAA |
NGG |
2 |
0.1202 |
Tier III |
| 5 |
NC_000011.10 |
11 |
41822808 |
+ |
LINC01499 |
NNNAAGGGCGATCTAATGTA |
NGC |
2 |
0.012 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
76637810 |
+ |
Sptb |
NNNAAGGGCGAACTAATGAG |
NGG |
2 |
0.6118 |
Tier II |
| 2 |
NC_000071.6 |
5 |
20336099 |
- |
Magi2 |
NNNAAGGGCAATCTAATGAT |
NGG |
2 |
0.56 |
Tier II |
| 3 |
NC_000084.6 |
18 |
25203549 |
- |
AW554918 |
NNNAAGGGTGATCTAGTGAA |
NGG |
2 |
0.1683 |
Tier II |
| 4 |
NC_000075.6 |
9 |
102776282 |
+ |
Gm46151 |
NNNAAGGTCGATCCAATGAA |
NGG |
2 |
0.1786 |
Tier III |
Other clones with same target sequence:
(none)
