Construct: sgRNA BRDN0001144969
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGATATCCAGAATCACGTAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DCAKD (79877)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75872
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
45030170 |
+ |
DCAKD |
NNNTATCCAGAATCACGTAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
51752539 |
+ |
CA10 |
NNNTATCCAGAATCACACAG |
NGG |
2 |
0.4 |
Tier II |
| 3 |
NC_000011.10 |
11 |
85857460 |
- |
CCDC83 |
NNNCATCCAGAATCACGTTG |
NGG |
2 |
0.3484 |
Tier II |
| 4 |
NC_000003.12 |
3 |
140281529 |
+ |
CLSTN2 |
NNNAATCCAGAAACACGTAG |
NGG |
2 |
0.3297 |
Tier II |
| 5 |
NC_000001.11 |
1 |
22530606 |
- |
ZBTB40 |
NNNTATACAGCATCACGTAG |
NGG |
2 |
0.3 |
Tier II |
| 6 |
NC_000015.10 |
15 |
71226650 |
- |
THSD4 |
NNNTATCCAGCATCACGAAG |
NGG |
2 |
0.2667 |
Tier II |
| 7 |
NC_000015.10 |
15 |
85649246 |
+ |
AKAP13 |
NNNTATCCAGAATTACTTAG |
NGG |
2 |
0.1833 |
Tier II |
| 8 |
NC_000011.10 |
11 |
85857460 |
- |
AHCYP6 |
NNNCATCCAGAATCACGTTG |
NGG |
2 |
0.3484 |
Tier III |
| 9 |
NC_000003.12 |
3 |
3744024 |
+ |
LOC100130207 |
NNNTAGCCAGAATCACTTAG |
NGG |
2 |
0.1429 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
102997431 |
+ |
Dcakd |
NNNTGTCCAGAATCACGTAT |
NGG |
2 |
0.504 |
Tier I |
| 2 |
NC_000084.6 |
18 |
21133753 |
+ |
Garem1 |
NNNTAACCATAATCACGTAG |
NGG |
2 |
0.7042 |
Tier II |
| 3 |
NC_000073.6 |
7 |
80213451 |
- |
Sema4b |
NNNTATCCAGGATCACATAG |
NGG |
2 |
0.6067 |
Tier II |
| 4 |
NC_000076.6 |
10 |
21158920 |
- |
Myb |
NNNTTTCTAGAATCACGTAG |
NGG |
2 |
0.3182 |
Tier II |
| 5 |
NC_000083.6 |
17 |
84774628 |
+ |
Lrpprc |
NNNTAGCCAGAATAACGTAG |
NGG |
2 |
0.2 |
Tier II |
| 6 |
NC_000081.6 |
15 |
59563953 |
+ |
Nsmce2 |
NNNTGTCCAGAATCACCTAG |
NGG |
2 |
0.1694 |
Tier II |
| 7 |
NC_000073.6 |
7 |
65019227 |
+ |
Fam189a1 |
NNNTATCCAGAATCACGGAC |
NGG |
2 |
0.1429 |
Tier II |
| 8 |
NC_000077.6 |
11 |
102997431 |
+ |
Gm34620 |
NNNTGTCCAGAATCACGTAT |
NGG |
2 |
0.504 |
Tier III |
| 9 |
NC_000081.6 |
15 |
34023518 |
+ |
Gm41290 |
NNNAACCCAGAATCACGTAG |
NGG |
2 |
0.4329 |
Tier III |
Other clones with same target sequence:
(none)
