Construct: sgRNA BRDN0001144970
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGCAGATGGAAGCTTACGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDC42BPB (9578)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77778
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
102975941 |
- |
CDC42BPB |
NNNAGATGGAAGCTTACGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
138404631 |
- |
MRAS |
NNNAGATGAAAGCTTACCAG |
NGG |
2 |
0.3061 |
Tier II |
| 3 |
NC_000002.12 |
2 |
236316504 |
+ |
IQCA1 |
NNNAGATGGAAGCTAAAGAG |
NGG |
2 |
0.2702 |
Tier II |
| 4 |
NC_000016.10 |
16 |
16216367 |
- |
ABCC6 |
NNNAGATGGAAGGTTACTAG |
NGG |
2 |
0.0909 |
Tier II |
| 5 |
NC_000017.11 |
17 |
33479777 |
- |
ASIC2 |
NNNAGATGAAAGCTTAGGAG |
NGG |
2 |
0.0378 |
Tier II |
| 6 |
NC_000008.11 |
8 |
11515164 |
+ |
BLK |
NNNAGATGGAAGCTCTCGAG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000014.9 |
14 |
50528305 |
+ |
MAP4K5 |
NNNAGATGGAAGCTTCAGAG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000016.10 |
16 |
18578309 |
+ |
ABCC6P1 |
NNNAGATGGAAGGTTACTAG |
NGG |
2 |
0.0909 |
Tier III |
| 9 |
NC_000018.10 |
18 |
22266425 |
- |
LOC105372018 |
NNNAGAAGGAAGCTTAGGAG |
NGG |
2 |
0.0515 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
69568319 |
- |
Wrap53 |
NNNAGATGGAAGCATATGAG |
NGG |
2 |
0.2889 |
Tier II |
| 2 |
NC_000082.6 |
16 |
58447483 |
- |
Dcbld2 |
NNNAGAGGGAAGCTCACGAG |
NGG |
2 |
0.1604 |
Tier II |
| 3 |
NC_000076.6 |
10 |
70280024 |
+ |
Slc16a9 |
NNNAGATGGATGCTTAAGAG |
NGG |
2 |
0.1436 |
Tier II |
| 4 |
NC_000083.6 |
17 |
26947093 |
- |
Syngap1 |
NNNAGATGGGAGCTTGCGAG |
NGG |
2 |
0.1068 |
Tier II |
| 5 |
NC_000075.6 |
9 |
99111868 |
+ |
Pik3cb |
NNNAGATGGAAGCTAAGGAG |
NGG |
2 |
0.0341 |
Tier II |
| 6 |
NC_000085.6 |
19 |
11810429 |
+ |
Stx3 |
NNNAGATGGAAGCTTATGAG |
NGA |
2 |
0.0324 |
Tier II |
| 7 |
NC_000072.6 |
6 |
125559567 |
+ |
Vwf |
NNNAGATGGAAGCTTAGGAG |
NAG |
2 |
0.0153 |
Tier II |
| 8 |
NC_000069.6 |
3 |
7683829 |
- |
Gm16685 |
NNNAGATGGAAGCTTATGAG |
NTG |
2 |
0.0182 |
Tier III |
| 9 |
NC_000072.6 |
6 |
126584585 |
- |
Gm33449 |
NNNAGATGGAAGCGTACGAG |
NGT |
2 |
0.0 |
Tier III |
| 10 |
NC_000073.6 |
7 |
84753200 |
+ |
2610206C17Rik |
NNNAGATGGAAGCTTCCCAG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
