Construct: sgRNA BRDN0001144972
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AACTTCTTAATAGGACGACC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CSNK1G3 (1456)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77363
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
123575806 |
+ |
CSNK1G3 |
NNNTTCTTAATAGGACGACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
6594370 |
- |
KLHL21 |
NNNTTCTGAATAGGACAACC |
NGG |
2 |
0.6844 |
Tier II |
| 3 |
NC_000001.11 |
1 |
64671810 |
- |
CACHD1 |
NNNTTCTTAATAGGACACCC |
NGG |
2 |
0.1778 |
Tier II |
| 4 |
NC_000002.12 |
2 |
119491916 |
+ |
SCTR |
NNNTTCTTACTAGGAGGACC |
NGG |
2 |
0.0513 |
Tier II |
| 5 |
NC_000018.10 |
18 |
67887154 |
+ |
DSEL-AS1 |
NNNTTCTTAATAGGAGAACC |
NGG |
2 |
0.1436 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
53918876 |
+ |
Csnk1g3 |
NNNTTCTTAATAGGACGGCC |
NGG |
1 |
0.4 |
Tier I |
| 2 |
NC_000078.6 |
12 |
86049433 |
- |
Ttll5 |
NNNTTCTTAATAGAACCACC |
NGG |
2 |
0.1765 |
Tier II |
| 3 |
NC_000086.7 |
X |
53418522 |
+ |
Gm14584 |
NNNTTCTTAATAGGACGGCC |
NGG |
1 |
0.4 |
Tier III |
| 4 |
NC_000084.6 |
18 |
80504918 |
- |
Gm30020 |
NNNTTCTTAATAGGATGACC |
NGG |
1 |
0.3077 |
Tier III |
Other clones with same target sequence:
(none)
