Construct: sgRNA BRDN0001144975
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTCCCACTAAAACATGCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PHKB (5257)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77847
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
47499817 |
+ |
PHKB |
NNNCCCACTAAAACATGCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
102949846 |
- |
PAH |
NNNTCCATTAAAACATGCGG |
NGG |
2 |
0.7 |
Tier II |
| 3 |
NC_000007.14 |
7 |
94925478 |
- |
PPP1R9A |
NNNCCCACTAAGACATGTGG |
NGG |
2 |
0.4643 |
Tier II |
| 4 |
NC_000008.11 |
8 |
1018350 |
- |
DLGAP2 |
NNNCCCAGTAAAACATGCTG |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000009.12 |
9 |
8915438 |
- |
PTPRD |
NNNCCCACTAATACATGCAG |
NGG |
2 |
0.2381 |
Tier II |
| 6 |
NC_000020.11 |
20 |
36912709 |
+ |
SAMHD1 |
NNNCCCACTAAAAAATGAGG |
NGG |
2 |
0.1885 |
Tier II |
| 7 |
NC_000018.10 |
18 |
11696891 |
+ |
GNAL |
NNNCCCACTAAAAGATGCAG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000012.12 |
12 |
102949846 |
- |
LOC112267865 |
NNNTCCATTAAAACATGCGG |
NGG |
2 |
0.7 |
Tier III |
| 9 |
NC_000021.9 |
21 |
14387217 |
- |
LOC105369304 |
NNNCCCTCTAAAACATGCAG |
NGG |
2 |
0.3125 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
85878171 |
+ |
Phkb |
NNNCCTACTAAAACATGTGG |
NGG |
2 |
0.5969 |
Tier I |
| 2 |
NC_000071.6 |
5 |
27663886 |
+ |
Dpp6 |
NNNCCAACTAAAACATGCTG |
NGG |
2 |
0.619 |
Tier II |
Other clones with same target sequence:
(none)
