Construct: sgRNA BRDN0001144978
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGACAATCGGACTCAGAAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK24 (8428)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77056
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
98519374 |
- |
STK24 |
NNNCAATCGGACTCAGAAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
58824037 |
+ |
MINDY2 |
NNNCAATAGGATTCAGAAAG |
NGG |
2 |
0.35 |
Tier II |
| 3 |
NC_000014.9 |
14 |
61820024 |
+ |
SYT16 |
NNNCAATCTGACTCAGAAAC |
NGG |
2 |
0.2286 |
Tier II |
| 4 |
NC_000020.11 |
20 |
46395705 |
+ |
ELMO2 |
NNNCCATCGGCCTCAGAAAG |
NGG |
2 |
0.2 |
Tier II |
| 5 |
NC_000006.12 |
6 |
145398946 |
- |
EPM2A |
NNNCAATTGGACTCAGAAAG |
NTG |
2 |
0.0341 |
Tier II |
| 6 |
NC_000010.11 |
10 |
99938624 |
- |
DNMBP |
NNNCAATCGGACTTAGAAAG |
NGT |
2 |
0.0118 |
Tier II |
| 7 |
NC_000023.11 |
X |
136296845 |
- |
STK24P1 |
NNNCAGTTGGACTCAGAAAG |
NGG |
2 |
0.625 |
Tier III |
| 8 |
NC_000008.11 |
8 |
128169776 |
- |
LOC124902020 |
NNNCAATGGGACCCAGAAAG |
NGG |
2 |
0.5075 |
Tier III |
| 9 |
NC_000020.11 |
20 |
46395705 |
+ |
LOC124904917 |
NNNCCATCGGCCTCAGAAAG |
NGG |
2 |
0.2 |
Tier III |
| 10 |
NC_000010.11 |
10 |
99938624 |
- |
DNMBP-AS1 |
NNNCAATCGGACTTAGAAAG |
NGT |
2 |
0.0118 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
121337553 |
- |
Stk24 |
NNNCAATCGGACTCAGAAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000071.6 |
5 |
121540533 |
- |
Mapkapk5 |
NNNCAATTGGACTCAGAAAC |
NGG |
2 |
0.375 |
Tier I |
| 3 |
NC_000070.6 |
4 |
46739612 |
+ |
Gabbr2 |
NNNAAATTGGACTCAGAAAG |
NGG |
2 |
0.7368 |
Tier II |
| 4 |
NC_000075.6 |
9 |
32125883 |
- |
Arhgap32 |
NNNAAATCAGACTCAGAAAG |
NGG |
2 |
0.5414 |
Tier II |
| 5 |
NC_000073.6 |
7 |
91039670 |
- |
Dlg2 |
NNNCAATAGTACTCAGAAAG |
NGG |
2 |
0.5281 |
Tier II |
| 6 |
NC_000081.6 |
15 |
83667428 |
+ |
Scube1 |
NNNCATTCGGACACAGAAAG |
NGG |
2 |
0.4945 |
Tier II |
| 7 |
NC_000069.6 |
3 |
98310407 |
- |
Hmgcs2 |
NNNCAATGGGACACAGAAAG |
NGG |
2 |
0.4451 |
Tier II |
| 8 |
NC_000070.6 |
4 |
40206850 |
+ |
Ddx58 |
NNNGAATCAGACTCAGAAAG |
NGG |
2 |
0.3214 |
Tier II |
| 9 |
NC_000069.6 |
3 |
54742410 |
+ |
Alg5 |
NNNCAAGCTGACTCAGAAAG |
NGG |
2 |
0.3137 |
Tier II |
| 10 |
NC_000077.6 |
11 |
67682381 |
- |
Gas7 |
NNNCACTAGGACTCAGAAAG |
NGG |
2 |
0.2955 |
Tier II |
| 11 |
NC_000077.6 |
11 |
67682381 |
- |
Glp2r |
NNNCACTAGGACTCAGAAAG |
NGG |
2 |
0.2955 |
Tier II |
| 12 |
NC_000068.7 |
2 |
170732741 |
- |
Dok5 |
NNNCAGTCGGACTCAGAAGG |
NGG |
2 |
0.2679 |
Tier II |
| 13 |
NC_000077.6 |
11 |
31902455 |
+ |
Cpeb4 |
NNNCAATGGGACTCAGAGAG |
NGG |
2 |
0.2571 |
Tier II |
| 14 |
NC_000083.6 |
17 |
49654089 |
+ |
Kif6 |
NNNCAATCCCACTCAGAAAG |
NGG |
2 |
0.2154 |
Tier II |
| 15 |
NC_000070.6 |
4 |
38629005 |
- |
Gm12381 |
NNNCAATCCTACTCAGAAAG |
NGG |
2 |
0.4375 |
Tier III |
Other clones with same target sequence:
(none)
