Construct: sgRNA BRDN0001144979

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATGGAATCTAGGTACGAGGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IGFN1 (91156)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76824

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 201207761 + IGFN1 NNNGAATCTAGGTACGAGGG NGG 0 1.0 Tier I
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 115839837 + Mknk1 NNNGAAGCTAGGTAAGAGGG NGG 2 0.1307 Tier II
2 NC_000073.6 7 27428928 - Sptbn4 NNNAAATCTAGGTACGTGGG NGG 2 0.12 Tier II
3 NC_000071.6 5 116270901 - Ccdc60 NNNGAATCTAGGTGCCAGGG NGG 2 0.0 Tier II
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Other clones with same target sequence:

(none)