Construct: sgRNA BRDN0001144981

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCCATGAATGAGGACCACCC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PHKG1 (5260)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76356

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 56082190 - PHKG1 NNNATGAATGAGGACCACCC NGG 0 1.0 Tier I
2 NC_000011.10 11 1094182 + MUC2 NNNATCAATGAGGACCACCC NAG 2 0.1768 Tier I
3 NC_000007.14 7 56082190 - SUMF2 NNNATGAATGAGGACCACCC NGG 0 1.0 Tier II
4 NC_000002.12 2 240553161 + ANKMY1 NNNATGAATGGGGACCACCC NGG 1 0.65 Tier II
5 NC_000001.11 1 44335413 - ERI3 NNNATGAATGAGAACCATCC NGG 2 0.5934 Tier II
6 NC_000019.10 19 15619471 - CYP4F8 NNNATGAATGAGGACCATCA NGG 2 0.3214 Tier II
7 NC_000002.12 2 9520013 + ADAM17 NNNATGAATGAGTACCACAC NGG 2 0.1286 Tier II
8 NC_000001.11 1 6087419 + KCNAB2 NNNATGAAGGAGGACCCCCC NGG 2 0.1092 Tier II
9 NC_000002.12 2 232229645 - DIS3L2 NNNATGTATGAGGAACACCC NGG 2 0.0972 Tier II
10 NC_000002.12 2 49014582 - FSHR NNNATGAATGAGGACCCCCT NGG 2 0.0529 Tier II
11 NC_000001.11 1 197297680 + CRB1 NNNATGATTGAGGATCACCC NGG 2 0.0286 Tier II
12 NC_000011.10 11 12279621 - MICAL2 NNNCTGAATGAGGACCACCC NTG 2 0.0138 Tier II
13 NC_000012.12 12 66191012 + IRAK3 NNNATGAATGAGGACCACCC NAA 2 0.0 Tier II
14 NC_000007.14 7 63234182 - PHKG1P1 NNNATGAATGAGGACCATCC NGG 1 0.6429 Tier III
15 NC_000011.10 11 50247697 - PHKG1P3 NNNATGAATGAGGACCATCC NGG 1 0.6429 Tier III
16 NC_000019.10 19 15774357 + CYP4F24P NNNATGAATGAGGACCATCA NGG 2 0.3214 Tier III
17 NC_000004.12 4 154191421 + LOC101927947 NNNCTCAATGAGGACCACCC NGG 2 0.2406 Tier III
18 NC_000003.12 3 187480055 + LOC124909471 NNNATGAATCAGGAGCACCC NGG 2 0.02 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 84230810 - Thada NNNATGAAGGAAGACCACCC NGG 2 0.5778 Tier I
2 NC_000082.6 16 34677199 + Ropn1 NNNATGATGGAGGACCACCC NGG 2 0.2653 Tier I
3 NC_000086.7 X 164533821 - Asb9 NNNATAAATGAGGACCACCC NGG 1 1.0 Tier II
4 NC_000075.6 9 55494408 + Etfa NNNATGAGTGAGGACAACCC NGG 2 0.7333 Tier II
5 NC_000070.6 4 63163595 - Kif12 NNNATGGATGGGGACCACCC NGG 2 0.4588 Tier II
6 NC_000067.6 1 86909743 - Dis3l2 NNNATCAATGAGGACCATCC NGG 2 0.4383 Tier II
7 NC_000078.6 12 117621696 - Rapgef5 NNNATGAATGAGCACAACCC NGG 2 0.4211 Tier II
8 NC_000072.6 6 90478808 + Klf15 NNNAAGAAGGAGGACCACCC NGG 2 0.3095 Tier II
9 NC_000078.6 12 34524456 - Hdac9 NNNATGGCTGAGGACCACCC NGG 2 0.3025 Tier II
10 NC_000070.6 4 128168460 - Csmd2 NNNATGAAGGAGGGCCACCC NGG 2 0.2889 Tier II
11 NC_000080.6 14 27712233 + Erc2 NNNATGCAGGAGGACCACCC NGG 2 0.2708 Tier II
12 NC_000072.6 6 137458990 + Ptpro NNNATGTATGAGGACCACCA NGG 2 0.2188 Tier II
13 NC_000067.6 1 43747619 + Uxs1 NNNATGACTGAGGACCACCA NGG 2 0.2143 Tier II
14 NC_000086.7 X 7316534 + Clcn5 NNNATGAATGTGCACCACCC NGG 2 0.1296 Tier II
15 NC_000081.6 15 68205860 + Zfat NNNATGAATGAGGACTACCC NAG 2 0.0798 Tier II
16 NC_000071.6 5 29777607 - Dnajb6 NNNATGAGTGAGGACCACCC NTG 2 0.0286 Tier II
17 NC_000086.7 X 57412124 - Gm364 NNNAGGAATGAGGACCACCC NTG 2 0.0249 Tier II
18 NC_000067.6 1 58023579 - Sgo2a NNNATGAATGAGGACTACCC NGC 2 0.0068 Tier II
19 NC_000078.6 12 29211399 - Gm31569 NNNATGATTGAGGATCACCC NGG 2 0.0286 Tier III
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Other clones with same target sequence:

(none)