Construct: sgRNA BRDN0001144981
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCCATGAATGAGGACCACCC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PHKG1 (5260)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76356
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000007.14 |
7 |
56082190 |
- |
PHKG1 |
NNNATGAATGAGGACCACCC |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000011.10 |
11 |
1094182 |
+ |
MUC2 |
NNNATCAATGAGGACCACCC |
NAG |
2 |
0.1768 |
Tier I |
3 |
NC_000007.14 |
7 |
56082190 |
- |
SUMF2 |
NNNATGAATGAGGACCACCC |
NGG |
0 |
1.0 |
Tier II |
4 |
NC_000002.12 |
2 |
240553161 |
+ |
ANKMY1 |
NNNATGAATGGGGACCACCC |
NGG |
1 |
0.65 |
Tier II |
5 |
NC_000001.11 |
1 |
44335413 |
- |
ERI3 |
NNNATGAATGAGAACCATCC |
NGG |
2 |
0.5934 |
Tier II |
6 |
NC_000019.10 |
19 |
15619471 |
- |
CYP4F8 |
NNNATGAATGAGGACCATCA |
NGG |
2 |
0.3214 |
Tier II |
7 |
NC_000002.12 |
2 |
9520013 |
+ |
ADAM17 |
NNNATGAATGAGTACCACAC |
NGG |
2 |
0.1286 |
Tier II |
8 |
NC_000001.11 |
1 |
6087419 |
+ |
KCNAB2 |
NNNATGAAGGAGGACCCCCC |
NGG |
2 |
0.1092 |
Tier II |
9 |
NC_000002.12 |
2 |
232229645 |
- |
DIS3L2 |
NNNATGTATGAGGAACACCC |
NGG |
2 |
0.0972 |
Tier II |
10 |
NC_000002.12 |
2 |
49014582 |
- |
FSHR |
NNNATGAATGAGGACCCCCT |
NGG |
2 |
0.0529 |
Tier II |
11 |
NC_000001.11 |
1 |
197297680 |
+ |
CRB1 |
NNNATGATTGAGGATCACCC |
NGG |
2 |
0.0286 |
Tier II |
12 |
NC_000011.10 |
11 |
12279621 |
- |
MICAL2 |
NNNCTGAATGAGGACCACCC |
NTG |
2 |
0.0138 |
Tier II |
13 |
NC_000012.12 |
12 |
66191012 |
+ |
IRAK3 |
NNNATGAATGAGGACCACCC |
NAA |
2 |
0.0 |
Tier II |
14 |
NC_000007.14 |
7 |
63234182 |
- |
PHKG1P1 |
NNNATGAATGAGGACCATCC |
NGG |
1 |
0.6429 |
Tier III |
15 |
NC_000011.10 |
11 |
50247697 |
- |
PHKG1P3 |
NNNATGAATGAGGACCATCC |
NGG |
1 |
0.6429 |
Tier III |
16 |
NC_000019.10 |
19 |
15774357 |
+ |
CYP4F24P |
NNNATGAATGAGGACCATCA |
NGG |
2 |
0.3214 |
Tier III |
17 |
NC_000004.12 |
4 |
154191421 |
+ |
LOC101927947 |
NNNCTCAATGAGGACCACCC |
NGG |
2 |
0.2406 |
Tier III |
18 |
NC_000003.12 |
3 |
187480055 |
+ |
LOC124909471 |
NNNATGAATCAGGAGCACCC |
NGG |
2 |
0.02 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000083.6 |
17 |
84230810 |
- |
Thada |
NNNATGAAGGAAGACCACCC |
NGG |
2 |
0.5778 |
Tier I |
2 |
NC_000082.6 |
16 |
34677199 |
+ |
Ropn1 |
NNNATGATGGAGGACCACCC |
NGG |
2 |
0.2653 |
Tier I |
3 |
NC_000086.7 |
X |
164533821 |
- |
Asb9 |
NNNATAAATGAGGACCACCC |
NGG |
1 |
1.0 |
Tier II |
4 |
NC_000075.6 |
9 |
55494408 |
+ |
Etfa |
NNNATGAGTGAGGACAACCC |
NGG |
2 |
0.7333 |
Tier II |
5 |
NC_000070.6 |
4 |
63163595 |
- |
Kif12 |
NNNATGGATGGGGACCACCC |
NGG |
2 |
0.4588 |
Tier II |
6 |
NC_000067.6 |
1 |
86909743 |
- |
Dis3l2 |
NNNATCAATGAGGACCATCC |
NGG |
2 |
0.4383 |
Tier II |
7 |
NC_000078.6 |
12 |
117621696 |
- |
Rapgef5 |
NNNATGAATGAGCACAACCC |
NGG |
2 |
0.4211 |
Tier II |
8 |
NC_000072.6 |
6 |
90478808 |
+ |
Klf15 |
NNNAAGAAGGAGGACCACCC |
NGG |
2 |
0.3095 |
Tier II |
9 |
NC_000078.6 |
12 |
34524456 |
- |
Hdac9 |
NNNATGGCTGAGGACCACCC |
NGG |
2 |
0.3025 |
Tier II |
10 |
NC_000070.6 |
4 |
128168460 |
- |
Csmd2 |
NNNATGAAGGAGGGCCACCC |
NGG |
2 |
0.2889 |
Tier II |
11 |
NC_000080.6 |
14 |
27712233 |
+ |
Erc2 |
NNNATGCAGGAGGACCACCC |
NGG |
2 |
0.2708 |
Tier II |
12 |
NC_000072.6 |
6 |
137458990 |
+ |
Ptpro |
NNNATGTATGAGGACCACCA |
NGG |
2 |
0.2188 |
Tier II |
13 |
NC_000067.6 |
1 |
43747619 |
+ |
Uxs1 |
NNNATGACTGAGGACCACCA |
NGG |
2 |
0.2143 |
Tier II |
14 |
NC_000086.7 |
X |
7316534 |
+ |
Clcn5 |
NNNATGAATGTGCACCACCC |
NGG |
2 |
0.1296 |
Tier II |
15 |
NC_000081.6 |
15 |
68205860 |
+ |
Zfat |
NNNATGAATGAGGACTACCC |
NAG |
2 |
0.0798 |
Tier II |
16 |
NC_000071.6 |
5 |
29777607 |
- |
Dnajb6 |
NNNATGAGTGAGGACCACCC |
NTG |
2 |
0.0286 |
Tier II |
17 |
NC_000086.7 |
X |
57412124 |
- |
Gm364 |
NNNAGGAATGAGGACCACCC |
NTG |
2 |
0.0249 |
Tier II |
18 |
NC_000067.6 |
1 |
58023579 |
- |
Sgo2a |
NNNATGAATGAGGACTACCC |
NGC |
2 |
0.0068 |
Tier II |
19 |
NC_000078.6 |
12 |
29211399 |
- |
Gm31569 |
NNNATGATTGAGGATCACCC |
NGG |
2 |
0.0286 |
Tier III |
Other clones with same target sequence:
(none)