Construct: sgRNA BRDN0001144982
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAGAAGGGAACAATGTCGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GCK (2645)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77581
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
44150005 |
- |
GCK |
NNNAAGGGAACAATGTCGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
21530830 |
+ |
ZNF385D |
NNNAAGGGAACAATGTCCTG |
NGG |
1 |
0.4762 |
Tier II |
| 3 |
NC_000009.12 |
9 |
123422021 |
- |
DENND1A |
NNNAAGGGAACAGAGTCGTG |
NGG |
2 |
0.4037 |
Tier II |
| 4 |
NC_000006.12 |
6 |
143821067 |
+ |
PHACTR2 |
NNNCAGGGAACAATGTCCTG |
NGG |
2 |
0.1681 |
Tier II |
| 5 |
NC_000006.12 |
6 |
146256818 |
- |
GRM1 |
NNNAAGGAAACAATTTCGTG |
NGG |
2 |
0.1429 |
Tier II |
| 6 |
NC_000005.10 |
5 |
81728001 |
+ |
SSBP2 |
NNNAAGGGAACACTGTCCTG |
NGG |
2 |
0.1003 |
Tier II |
| 7 |
NC_000003.12 |
3 |
29487280 |
+ |
RBMS3 |
NNNAAGGGAATAATCTCGTG |
NGG |
2 |
0.0839 |
Tier II |
| 8 |
NC_000001.11 |
1 |
111873156 |
- |
KCND3 |
NNNAAGGGAACAATGTCGGG |
NAG |
2 |
0.0648 |
Tier II |
| 9 |
NC_000003.12 |
3 |
168740760 |
- |
EGFEM1P |
NNNAAGGGAACAAGGTAGTG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
12473268 |
+ |
Dtx4 |
NNNAAGGTAACAATGTCGTG |
NGA |
2 |
0.0434 |
Tier I |
| 2 |
NC_000075.6 |
9 |
111153242 |
- |
Lrrfip2 |
NNNGAGGAAACAATGTCGTG |
NGG |
2 |
0.625 |
Tier II |
| 3 |
NC_000073.6 |
7 |
117419824 |
+ |
Xylt1 |
NNNAAGGGATCAATGTCTTG |
NGG |
2 |
0.5882 |
Tier II |
| 4 |
NC_000076.6 |
10 |
127804260 |
- |
Rdh16 |
NNNAAGGCAACAATGTCATG |
NGG |
2 |
0.426 |
Tier II |
| 5 |
NC_000082.6 |
16 |
17680788 |
+ |
Med15 |
NNNAAGGGCACAATGTCTTG |
NGG |
2 |
0.381 |
Tier II |
| 6 |
NC_000070.6 |
4 |
140997708 |
- |
Atp13a2 |
NNNATGGGAACAATGTCGTG |
NGG |
1 |
0.3636 |
Tier II |
| 7 |
NC_000067.6 |
1 |
93050116 |
- |
Kif1a |
NNNCAGGGAACAATGACGTG |
NGG |
2 |
0.3209 |
Tier II |
| 8 |
NC_000067.6 |
1 |
87326927 |
+ |
Gigyf2 |
NNNAAGGGAACGATGTCGTG |
NGA |
2 |
0.0502 |
Tier II |
| 9 |
NC_000067.6 |
1 |
166632045 |
- |
Gm4847 |
NNNAAGGGAACAATGGCGTG |
NGA |
2 |
0.024 |
Tier II |
| 10 |
NC_000068.7 |
2 |
20745797 |
- |
Etl4 |
NNNATGGGAACAATGTCGTG |
NGC |
2 |
0.0081 |
Tier II |
| 11 |
NC_000070.6 |
4 |
56270553 |
- |
Gm42291 |
NNNAAGGGAACAATGGAGTG |
NGG |
2 |
0.1615 |
Tier III |
| 12 |
NC_000080.6 |
14 |
51061323 |
- |
Gm52088 |
NNNAAGGGAAGAATGTCCTG |
NGG |
2 |
0.119 |
Tier III |
| 13 |
NC_000075.6 |
9 |
94471643 |
- |
Gm46148 |
NNNAAGGGAACAAAGTCGTG |
NGA |
2 |
0.043 |
Tier III |
Other clones with same target sequence:
(none)
