Construct: sgRNA BRDN0001144984
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAATGGGCAACCAGTGTTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKAR1A (5573)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77721
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
68525785 |
+ |
PRKAR1A |
NNNATGGGCAACCAGTGTTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
85105181 |
+ |
PDE8A |
NNNATGGGCTACCAGGGTTG |
NGG |
2 |
0.3054 |
Tier II |
| 3 |
NC_000012.12 |
12 |
46796859 |
- |
SLC38A4 |
NNNATGGGCAACCAGTGATC |
NGG |
2 |
0.2857 |
Tier II |
| 4 |
NC_000001.11 |
1 |
92125905 |
+ |
BTBD8 |
NNNATGAGCAACCAGTGTTG |
NAG |
2 |
0.2593 |
Tier II |
| 5 |
NC_000003.12 |
3 |
77366736 |
+ |
ROBO2 |
NNNATGGGGAACCAGTGTAG |
NGG |
2 |
0.1769 |
Tier II |
| 6 |
NC_000008.11 |
8 |
90860288 |
+ |
NECAB1 |
NNNATGGGCAGCCAGTTTTG |
NGG |
2 |
0.1625 |
Tier II |
| 7 |
NC_000022.11 |
22 |
20737014 |
+ |
PI4KA |
NNNATGGGCGACCAGTGTAG |
NGG |
2 |
0.1587 |
Tier II |
| 8 |
NC_000015.10 |
15 |
28048409 |
+ |
OCA2 |
NNNATGGCCAACCAGTTTTG |
NGG |
2 |
0.1538 |
Tier II |
| 9 |
NC_000006.12 |
6 |
108432877 |
+ |
AFG1L |
NNNATGGGCAGCCAGTGTTG |
NGA |
2 |
0.0451 |
Tier II |
| 10 |
NC_000001.11 |
1 |
167086001 |
+ |
GPA33 |
NNNATGGGCAACCAGGGTTG |
NGA |
2 |
0.024 |
Tier II |
| 11 |
NC_000009.12 |
9 |
74730967 |
- |
TRPM6 |
NNNATGGGCAACCAGGGTTG |
NGA |
2 |
0.024 |
Tier II |
| 12 |
NC_000023.11 |
X |
122461249 |
- |
LOC101928359 |
NNNATGTGAAACCAGTGTTG |
NGG |
2 |
0.4898 |
Tier III |
| 13 |
NC_000001.11 |
1 |
92125905 |
+ |
PRKAR1AP1 |
NNNATGAGCAACCAGTGTTG |
NAG |
2 |
0.2593 |
Tier III |
| 14 |
NC_000008.11 |
8 |
90860288 |
+ |
LOC105375634 |
NNNATGGGCAGCCAGTTTTG |
NGG |
2 |
0.1625 |
Tier III |
| 15 |
NC_000015.10 |
15 |
36460496 |
- |
LOC105370768 |
NNNATGTGCAACCAGTTTTG |
NGG |
2 |
0.1429 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
109662980 |
+ |
Prkar1a |
NNNATGGGCAACCAGTGTTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000080.6 |
14 |
103130275 |
- |
Mycbp2 |
NNNATGGGAAAACAGTGTTG |
NGG |
2 |
0.6122 |
Tier II |
| 3 |
NC_000086.7 |
X |
84405193 |
- |
Dmd |
NNNGTGGGCAACCAATGTTG |
NGG |
2 |
0.5882 |
Tier II |
| 4 |
NC_000075.6 |
9 |
118724967 |
+ |
Itga9 |
NNNATGGGGAGCCAGTGTTG |
NGG |
2 |
0.4024 |
Tier II |
| 5 |
NC_000086.7 |
X |
75193841 |
- |
F8 |
NNNATGGGGAACCTGTGTTG |
NGG |
2 |
0.3302 |
Tier II |
| 6 |
NC_000074.6 |
8 |
118833723 |
+ |
Cdh13 |
NNNATGGGCAAACAGTGGTG |
NGG |
2 |
0.2381 |
Tier II |
| 7 |
NC_000073.6 |
7 |
124378270 |
+ |
Hs3st4 |
NNNATGGGCAGCCAGTGTTG |
NGA |
2 |
0.0451 |
Tier II |
| 8 |
NC_000085.6 |
19 |
23219817 |
- |
Smc5 |
NNNTTGGGCAACCAGTGTTG |
NTG |
2 |
0.0248 |
Tier II |
| 9 |
NC_000075.6 |
9 |
65201001 |
- |
Gm40527 |
NNNATTGGCAACCAGTATTG |
NGG |
2 |
0.6222 |
Tier III |
| 10 |
NC_000069.6 |
3 |
73339078 |
+ |
Gm20754 |
NNNATTGGCAACCAGTCTTG |
NGG |
2 |
0.1569 |
Tier III |
| 11 |
NC_000072.6 |
6 |
6334608 |
- |
Gm20619 |
NNNATGGGGAACCAGTCTTG |
NGG |
2 |
0.1457 |
Tier III |
Other clones with same target sequence:
(none)
