Construct: sgRNA BRDN0001144986
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTGAACGAATCAAGACCCT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKACA (5566)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75515
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
14106851 |
- |
PRKACA |
NNNGAACGAATCAAGACCCT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
58037235 |
- |
VRK2 |
NNNAAACGAATCAAGACACT |
NGG |
2 |
0.4846 |
Tier II |
| 3 |
NC_000013.11 |
13 |
24432869 |
+ |
PARP4 |
NNNGACAGAATCAAGACCCT |
NGG |
2 |
0.3409 |
Tier II |
| 4 |
NC_000015.10 |
15 |
29574785 |
+ |
ENTREP2 |
NNNGAAAGAATCTAGACCCT |
NGG |
2 |
0.225 |
Tier II |
| 5 |
NC_000019.10 |
19 |
34932283 |
- |
ZNF30 |
NNNGAACAAATCAAGACCCT |
NTG |
2 |
0.039 |
Tier II |
| 6 |
NC_000013.11 |
13 |
114049595 |
+ |
RASA3 |
NNNGAACGAATCAAGTCCCT |
NTG |
2 |
0.0 |
Tier II |
| 7 |
NC_000006.12 |
6 |
87903538 |
- |
LOC101928911 |
NNNGAACGAATCAAGATCCT |
NAG |
2 |
0.121 |
Tier III |
| 8 |
NC_000006.12 |
6 |
121790756 |
+ |
LOC105377979 |
NNNGCACGAATCAAGACCCT |
NGA |
2 |
0.0347 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
83981210 |
+ |
Prkaca |
NNNGATAGAATCAAGACCCT |
NGG |
2 |
0.5357 |
Tier I |
| 2 |
NC_000075.6 |
9 |
123082099 |
+ |
Zdhhc3 |
NNNGAACGAATCAAGGCCCA |
NGG |
2 |
0.1082 |
Tier II |
| 3 |
NC_000076.6 |
10 |
3826397 |
- |
Plekhg1 |
NNNGAACGATTCAAGACCCT |
NTG |
2 |
0.0344 |
Tier II |
| 4 |
NC_000077.6 |
11 |
98700590 |
- |
Csf3 |
NNNGAATGAATCAAGACCCT |
NTG |
2 |
0.0317 |
Tier II |
| 5 |
NC_000086.7 |
X |
161486366 |
- |
Prkaca-ps1 |
NNNGATAGAATCAAGACCCT |
NGG |
2 |
0.5357 |
Tier III |
| 6 |
NC_000077.6 |
11 |
98700590 |
- |
Gm38985 |
NNNGAATGAATCAAGACCCT |
NTG |
2 |
0.0317 |
Tier III |
| 7 |
NC_000081.6 |
15 |
51243411 |
- |
Gm19303 |
NNNGAACGATTCAAGCCCCT |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
