Construct: sgRNA BRDN0001144988
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTATGACAAGATTACCGTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IPMK (253430)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78076
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
58227044 |
+ |
IPMK |
NNNATGACAAGATTACCGTT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
4702943 |
+ |
CSMD1 |
NNNATGACAAAATTACCCTT |
NGG |
2 |
0.4762 |
Tier II |
| 3 |
NC_000007.14 |
7 |
149880701 |
+ |
ATP6V0E2 |
NNNATGAAAAGATTACTGTT |
NGG |
2 |
0.3033 |
Tier II |
| 4 |
NC_000005.10 |
5 |
94728726 |
- |
MCTP1 |
NNNATGAAAATATTACCGTT |
NGG |
2 |
0.25 |
Tier II |
| 5 |
NC_000018.10 |
18 |
62736500 |
+ |
PHLPP1 |
NNNATGACAAGTTTACAGTT |
NGG |
2 |
0.1556 |
Tier II |
| 6 |
NC_000017.11 |
17 |
59548014 |
- |
LOC105371847 |
NNNATGACAAGTTTAACGTT |
NGG |
2 |
0.3333 |
Tier III |
| 7 |
NC_000011.10 |
11 |
30194361 |
- |
ARL14EP-DT |
NNNATGACAAGGTTACGGTT |
NGG |
2 |
0.0425 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
157709320 |
+ |
Smpx |
NNNATGACAAGATTACAGTT |
NAG |
2 |
0.121 |
Tier II |
| 2 |
NC_000067.6 |
1 |
73393787 |
- |
Pinc |
NNNATGAGAAAATTACCGTT |
NGG |
2 |
0.6429 |
Tier III |
| 3 |
NC_000076.6 |
10 |
26445418 |
+ |
Gm30034 |
NNNATGACAACATTAACGTT |
NGG |
2 |
0.4286 |
Tier III |
| 4 |
NC_000071.6 |
5 |
46360713 |
- |
Gm7931 |
NNNTTGACAAGATTACCTTT |
NGG |
2 |
0.4242 |
Tier III |
Other clones with same target sequence:
(none)
