Construct: sgRNA BRDN0001144989
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGTCATGATGTAGCGATAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TRPM6 (140803)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77334
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
74776020 |
+ |
TRPM6 |
NNNTCATGATGTAGCGATAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
29330764 |
+ |
ENTREP2 |
NNNTCATAATGTAGCGATAT |
NGG |
2 |
0.7 |
Tier II |
| 3 |
NC_000008.11 |
8 |
98678645 |
+ |
STK3 |
NNNTCAAGATGTAGTGATAG |
NGG |
2 |
0.0583 |
Tier II |
| 4 |
NC_000008.11 |
8 |
112643085 |
+ |
CSMD3 |
NNNTCATGATCTAGGGATAG |
NGG |
2 |
0.0214 |
Tier II |
| 5 |
NC_000006.12 |
6 |
88388448 |
+ |
LOC105377885 |
NNNTATTGATGTAGCGATAG |
NGG |
2 |
0.4082 |
Tier III |
| 6 |
NC_000018.10 |
18 |
31725307 |
- |
LRRC37A7P |
NNNTCATGATGTATCAATAG |
NGG |
2 |
0.2667 |
Tier III |
| 7 |
NC_000004.12 |
4 |
138341196 |
- |
LINC00499 |
NNNTCATGATATAGCGTTAG |
NGG |
2 |
0.1333 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
18832526 |
- |
Trpm6 |
NNNTCATGATGTAGCGATAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000081.6 |
15 |
39346251 |
+ |
Rims2 |
NNNTCATGATGTAGCACTAG |
NGG |
2 |
0.1765 |
Tier II |
| 3 |
NC_000068.7 |
2 |
134634358 |
+ |
Tmx4 |
NNNTCATGATCTAGAGATAG |
NGG |
2 |
0.0952 |
Tier II |
| 4 |
NC_000078.6 |
12 |
111861136 |
- |
Ppp1r13b |
NNNTCATGATGTAGAGATGG |
NGG |
2 |
0.0833 |
Tier II |
| 5 |
NC_000078.6 |
12 |
5314408 |
+ |
Klhl29 |
NNNTCCTGATGTAGCGTTAG |
NGG |
2 |
0.0606 |
Tier II |
| 6 |
NC_000070.6 |
4 |
77795451 |
- |
Ptprd |
NNNTCAGGATGTAGGGATAG |
NGG |
2 |
0.0294 |
Tier II |
| 7 |
NC_000068.7 |
2 |
34988212 |
- |
Hc |
NNNTCATGATTTAGGGATAG |
NGG |
2 |
0.0192 |
Tier II |
| 8 |
NC_000078.6 |
12 |
111861136 |
- |
Gm19195 |
NNNTCATGATGTAGAGATGG |
NGG |
2 |
0.0833 |
Tier III |
| 9 |
NC_000072.6 |
6 |
138876646 |
+ |
Gm30583 |
NNNTCATGATGTAGCAATAG |
NTG |
2 |
0.039 |
Tier III |
Other clones with same target sequence:
(none)
