Construct: sgRNA BRDN0001144990
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCACAGTGGTCACAAAACGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKCB (5579)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75955
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
24032187 |
- |
PRKCB |
NNNCAGTGGTCACAAAACGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
38501167 |
+ |
RASGRP1 |
NNNCAGTTGTCACAAAAAGT |
NGG |
2 |
0.3365 |
Tier I |
| 3 |
NC_000003.12 |
3 |
53183521 |
- |
PRKCD |
NNNCAGTGGTCACAGAAGGT |
NGG |
2 |
0.0867 |
Tier I |
| 4 |
NC_000009.12 |
9 |
130492450 |
- |
ASS1 |
NNNCAGTGGTCACAGAACCT |
NGG |
2 |
0.2914 |
Tier II |
| 5 |
NC_000019.10 |
19 |
41712981 |
- |
CEACAM5 |
NNNCAGTGGTCAAAAAATGT |
NGG |
2 |
0.2473 |
Tier II |
| 6 |
NC_000014.9 |
14 |
60808747 |
+ |
MNAT1 |
NNNCAGTGGTCACAAAACAC |
NGG |
2 |
0.0649 |
Tier II |
| 7 |
NC_000012.12 |
12 |
3254882 |
- |
TSPAN9 |
NNNCAGTGGTCACAAACCGT |
NGA |
2 |
0.0123 |
Tier II |
| 8 |
NC_000004.12 |
4 |
42318444 |
- |
LOC105374428 |
NNNCAGTGGTCACAGAACAT |
NGG |
2 |
0.4643 |
Tier III |
| 9 |
NC_000020.11 |
20 |
1521534 |
+ |
LOC105372498 |
NNNCAGTGGTCACAAAAGGT |
NGG |
1 |
0.1333 |
Tier III |
| 10 |
NC_000020.11 |
20 |
1521534 |
+ |
LOC105372499 |
NNNCAGTGGTCACAAAAGGT |
NGG |
1 |
0.1333 |
Tier III |
| 11 |
NC_000002.12 |
2 |
220435147 |
- |
LOC105373893 |
NNNCAGTGGTCACAAGAGGT |
NGG |
2 |
0.0256 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
73697986 |
- |
Prkch |
NNNCAGTGGTCACAGAACGT |
NGG |
1 |
0.65 |
Tier I |
| 2 |
NC_000083.6 |
17 |
86482189 |
- |
Prkce |
NNNCAGTGGTCACAGAACGT |
NGG |
1 |
0.65 |
Tier I |
| 3 |
NC_000073.6 |
7 |
122457266 |
- |
Prkcb |
NNNCAGTGGTCACAGAAGGT |
NGG |
2 |
0.0867 |
Tier I |
| 4 |
NC_000080.6 |
14 |
30605501 |
+ |
Prkcd |
NNNCAGTGGTCACAGAAGGT |
NGG |
2 |
0.0867 |
Tier I |
| 5 |
NC_000078.6 |
12 |
72406222 |
- |
Rtn1 |
NNNTACTGGTCACAAAACGT |
NGG |
2 |
0.5455 |
Tier II |
| 6 |
NC_000073.6 |
7 |
130410912 |
+ |
Ate1 |
NNNCAGTGGTCACAAAACCT |
NGG |
1 |
0.4483 |
Tier II |
| 7 |
NC_000082.6 |
16 |
42895979 |
- |
Zbtb20 |
NNNCAGTCATCACAAAACGT |
NGG |
2 |
0.3956 |
Tier II |
| 8 |
NC_000067.6 |
1 |
159801556 |
- |
Tnr |
NNNCAGTGGTCACACAACAT |
NGG |
2 |
0.1948 |
Tier II |
| 9 |
NC_000077.6 |
11 |
43073359 |
- |
Atp10b |
NNNCAGTGGTCACAAGACGT |
NTG |
2 |
0.0075 |
Tier II |
| 10 |
NC_000067.6 |
1 |
86770627 |
+ |
Dis3l2 |
NNNCAGTGGTCACAACACTT |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000067.6 |
1 |
132650936 |
+ |
Nfasc |
NNNCAGTGGTCACAATATGT |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000082.6 |
16 |
42895979 |
- |
Gm19522 |
NNNCAGTCATCACAAAACGT |
NGG |
2 |
0.3956 |
Tier III |
| 13 |
NC_000073.6 |
7 |
138539036 |
+ |
Gm30751 |
NNNCAATGGTCACAAAAGGT |
NGG |
2 |
0.1333 |
Tier III |
Other clones with same target sequence:
(none)
