Construct: sgRNA BRDN0001144994
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTAATTGGTATGAGCTAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHA4 (2043)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76666
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
221482459 |
+ |
EPHA4 |
NNNAATTGGTATGAGCTAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
181640251 |
- |
CERKL |
NNNAATTGGTATAAGCTAGG |
NGG |
1 |
0.9231 |
Tier II |
| 3 |
NC_000006.12 |
6 |
128978821 |
+ |
LAMA2 |
NNNAATTAGTAAGAGCTAGG |
NGG |
2 |
0.8 |
Tier II |
| 4 |
NC_000003.12 |
3 |
184253151 |
+ |
EEF1AKMT4 |
NNNAATTGGTAAGAGCTAGA |
NGG |
2 |
0.75 |
Tier II |
| 5 |
NC_000003.12 |
3 |
184253151 |
+ |
EEF1AKMT4-ECE2 |
NNNAATTGGTAAGAGCTAGA |
NGG |
2 |
0.75 |
Tier II |
| 6 |
NC_000012.12 |
12 |
122318684 |
+ |
CLIP1 |
NNNGGTTGGTATGAGCTAGG |
NGG |
2 |
0.45 |
Tier II |
| 7 |
NC_000009.12 |
9 |
68771938 |
+ |
PIP5K1B |
NNNAATTTGTATGAGGTAGG |
NGG |
2 |
0.0962 |
Tier II |
| 8 |
NC_000014.9 |
14 |
48805954 |
- |
LOC105378178 |
NNNAAATGGTATGAGCTAGG |
NAG |
2 |
0.2247 |
Tier III |
| 9 |
NC_000003.12 |
3 |
73826644 |
- |
LINC02005 |
NNNAATTGGTATGAGCGAGG |
NGG |
1 |
0.1176 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
63652322 |
+ |
Epha3 |
NNNAGTTGGTGTGAGCTAGG |
NGG |
2 |
0.468 |
Tier I |
| 2 |
NC_000067.6 |
1 |
77426658 |
+ |
Epha4 |
NNNAATTGGTGTGTGCTAGG |
NGG |
2 |
0.3467 |
Tier I |
| 3 |
NC_000075.6 |
9 |
53420348 |
+ |
4930550C14Rik |
NNNAATGGGTATGGGCTAGG |
NGG |
2 |
0.2745 |
Tier II |
| 4 |
NC_000081.6 |
15 |
64856025 |
+ |
Adcy8 |
NNNAATAGGTATGAGCTAGG |
NAG |
2 |
0.2269 |
Tier II |
| 5 |
NC_000078.6 |
12 |
36287170 |
+ |
Gm40392 |
NNNAATGGGGATGAGCTAGG |
NGG |
2 |
0.2941 |
Tier III |
Other clones with same target sequence:
(none)
