GPP Web Portal - Clone Details

Construct: sgRNA BRDN0001145000

Construct Description:

Construct Type:: sgRNA
Other Identifiers:: n/a
DNA Barcode:: TGAGGAAATTCAATGCAAGG

Original Target:

Taxon:: Homo sapiens (human)
Gene:: CASK (8573)
Gene Description:: n/a
CRISPR Mechanism: CRISPRko

Vector Information:

Vector Backbone:: pXPR_003
Pol II Cassette 1:: EF1A-PuroR
Pol II Cassette 2:: n/a
Selection Marker:: PuroR
Visible Reporter:: n/a
Epitope Tag:: n/a

Additional Information:

Addgene ID:: 77402

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000023.11	X	41636593	-	CASK	NNNGGAAATTCAATGCAAGG	NGG	0	1.0	Tier I
2	NC_000002.12	2	40395836	+	SLC8A1	NNNGGAAATTCAATGCAAGA	NGG	1	0.9375	Tier II
3	NC_000001.11	1	210373757	-	HHAT	NNNACAAATTCAATGCAAGG	NGG	2	0.7071	Tier II
4	NC_000010.11	10	77544581	-	KCNMA1	NNNGGAAATTCAGTGAAAGG	NGG	2	0.6522	Tier II
5	NC_000005.10	5	54201962	+	ARL15	NNNGGAAATTCAAAGCAAGA	NGG	2	0.5804	Tier II
6	NC_000023.11	X	132759126	+	HS6ST2	NNNGGAAATTAAATGCAAAG	NGG	2	0.5357	Tier II
7	NC_000022.11	22	26018429	-	MYO18B	NNNGGAAGTTCAAAGCAAGG	NGG	2	0.454	Tier II
8	NC_000006.12	6	72199019	+	RIMS1	NNNAGAAATTCAATGCAAGG	NAG	2	0.2333	Tier II
9	NC_000005.10	5	75946044	+	SV2C	NNNTGAAATTCAAAGCAAGG	NGG	2	0.2251	Tier II
10	NC_000023.11	X	72262516	+	PIN4	NNNGGAAATTCACTGCAAGG	NGG	1	0.2105	Tier II
11	NC_000008.11	8	105609611	-	ZFPM2	NNNGGGAATTCAACGCAAGG	NGG	2	0.2041	Tier II
12	NC_000014.9	14	22914655	+	RBM23	NNNGGAATTTCTATGCAAGG	NGG	2	0.1429	Tier II
13	NC_000003.12	3	81582636	+	GBE1	NNNAGAAATTCAATGGAAGG	NGG	2	0.1385	Tier II
14	NC_000006.12	6	108348461	-	AFG1L	NNNGGACATTCAATGCAAGG	NAG	2	0.1134	Tier II
15	NC_000002.12	2	65086178	+	CEP68	NNNGGAAATTCAATCCAAGG	NCG	2	0.0292	Tier II
16	NC_000002.12	2	9974697	+	GRHL1	NNNGGAAATTCTATGCAAGG	NGA	2	0.0231	Tier II
17	NC_000005.10	5	11502354	+	CTNND2	NNNGGAAATTCAATTCAAGG	NGT	2	0.0023	Tier II
18	NC_000023.11	X	41636593	-	LOC124905180	NNNGGAAATTCAATGCAAGG	NGG	0	1.0	Tier III
19	NC_000002.12	2	40395836	+	LOC124905995	NNNGGAAATTCAATGCAAGA	NGG	1	0.9375	Tier III
20	NC_000005.10	5	74529239	-	LINC01331	NNNGGAAATTCATTGCAAGG	NGG	1	0.3	Tier III
21	NC_000008.11	8	63442518	+	LOC105375874	NNNGAAAATTCAATCCAAGG	NGG	2	0.2364	Tier III
22	NC_000005.10	5	83393428	+	LOC105379053	NNNGGAAATTCACTGCAAAG	NGG	2	0.1504	Tier III
23	NC_000005.10	5	113693449	-	LOC107986441	NNNGGAAATTCACAGCAAGG	NGG	2	0.1303	Tier III
24	NC_000011.10	11	10378489	+	CAND1.11	NNNGGAAATTCTTTGCAAGG	NGG	2	0.1	Tier III
25	NC_000003.12	3	187203652	+	LOC101929106	NNNGGAAATCCAATGGAAGG	NGG	2	0.0821	Tier III
26	NC_000010.11	10	58459634	-	LOC105378316	NNNGGAAATTCAATGCATGG	NGC	2	0.0111	Tier III

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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000077.6	11	5988970	-	Camk2b	NNNAGAAGTTCAATGCAAGG	NGG	2	0.66	Tier I
2	NC_000070.6	4	45041875	-	Fbxo10	NNNGGAATTTCAGTGCAAGG	NGG	2	0.2795	Tier I
3	NC_000086.7	X	13608749	-	Cask	NNNGGAAATTCAATGCAAGG	NGA	1	0.0694	Tier I
4	NC_000085.6	19	30994570	-	Prkg1	NNNGGAAATTCAAAGCAAGT	NGG	2	0.4333	Tier II
5	NC_000075.6	9	21457275	+	Dnm2	NNNTGAAATACAATGCAAGG	NGG	2	0.3117	Tier II
6	NC_000073.6	7	134973238	+	Dock1	NNNTGAAATTCAATGCAAGT	NGG	2	0.2545	Tier II
7	NC_000083.6	17	78679565	+	Strn	NNNGGGAATTGAATGCAAGG	NGG	2	0.1786	Tier II
8	NC_000085.6	19	55321558	-	Vti1a	NNNGGAAATTCAATCCAAGG	NAG	2	0.0707	Tier II
9	NC_000071.6	5	70998085	-	Gabra2	NNNTGAAATTCAATTCAAGG	NGG	2	0.0519	Tier II
10	NC_000069.6	3	55290415	-	Dclk1	NNNGGAACTTCAATGCAAGG	NTG	2	0.0167	Tier II
11	NC_000070.6	4	36441990	+	Lingo2	NNNGGAAATTTAATGCAAGG	NTG	2	0.012	Tier II
12	NC_000069.6	3	25565231	-	Nlgn1	NNNGGAAATTCAATCCAAGG	NGT	2	0.0044	Tier II
13	NC_000070.6	4	106487836	+	Bsnd	NNNGAAAATTCAAGGCAAGG	NGG	2	0.0	Tier II
14	NC_000076.6	10	96538187	+	Gm33843	NNNGGAAATTCAAAGCAAGG	NAG	2	0.1605	Tier III
15	NC_000075.6	9	88862320	-	Gm26611	NNNGGAAATTGAATCCAAGG	NGG	2	0.0682	Tier III

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Other clones with same target sequence:

(none)