Construct: sgRNA BRDN0001145005
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCAGAATACGTACACCAATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- KALRN (8997)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77639
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
124384858 |
+ |
KALRN |
NNNGAATACGTACACCAATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
14336549 |
+ |
TRIO |
NNNGAACACATACACCAATG |
NGG |
2 |
0.6417 |
Tier I |
| 3 |
NC_000020.11 |
20 |
42629871 |
+ |
PTPRT |
NNNGAACACCTACACCAATG |
NGG |
2 |
0.275 |
Tier II |
| 4 |
NC_000001.11 |
1 |
43831018 |
- |
ST3GAL3 |
NNNGAATACCTACAGCAATG |
NGG |
2 |
0.02 |
Tier II |
| 5 |
NC_000004.12 |
4 |
187385944 |
- |
LOC339975 |
NNNGAATATGTACACCAATT |
NGG |
2 |
0.6125 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
34262807 |
- |
Kalrn |
NNNGAATACATACACCAATG |
NGG |
1 |
0.9333 |
Tier I |
| 2 |
NC_000080.6 |
14 |
67921542 |
- |
Dock5 |
NNNGAATAAATACACCAATG |
NGG |
2 |
0.8 |
Tier II |
| 3 |
NC_000075.6 |
9 |
42773194 |
+ |
Grik4 |
NNNGAATACGTACACAAAAG |
NGG |
2 |
0.2857 |
Tier II |
| 4 |
NC_000085.6 |
19 |
41328406 |
+ |
Pik3ap1 |
NNNGAATACGTACACCAAAT |
NGG |
2 |
0.2 |
Tier II |
Other clones with same target sequence:
(none)
