Construct: sgRNA BRDN0001145020

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACAGGAAATGCATTCTACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP4K3 (8491)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76405

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 39288244 - MAP4K3 NNNGGAAATGCATTCTACTG NGG 0 1.0 Tier I
2 NC_000006.12 6 63880042 + EYS NNNGGAAACTCATTCTACTG NGG 2 0.75 Tier II
3 NC_000024.10 Y 14807582 + NLGN4Y NNNGGAAATGCACTCAACTG NGG 2 0.7177 Tier II
4 NC_000003.12 3 15022353 + NR2C2 NNNGGAAATGCATTCTATTA NGG 2 0.6027 Tier II
5 NC_000001.11 1 181467735 + CACNA1E NNNGCAAATGCATTCCACTG NGG 2 0.5238 Tier II
6 NC_000005.10 5 176243108 + SIMC1 NNNGGAACTACATTCTACTG NGG 2 0.4 Tier II
7 NC_000012.12 12 102053030 - WASHC3 NNNGGCAATGCAATCTACTG NGG 2 0.3147 Tier II
8 NC_000015.10 15 55827306 - NEDD4 NNNTCAAATGCATTCTACTG NGG 2 0.2857 Tier II
9 NC_000001.11 1 180200507 - QSOX1 NNNGGAAATCCATACTACTG NGG 2 0.2476 Tier II
10 NC_000012.12 12 8837976 - A2ML1 NNNGGAAATGCTTTCTACTT NGG 2 0.2333 Tier II
11 NC_000015.10 15 24902289 + SNRPN NNNGGCTATGCATTCTACTG NGG 2 0.1989 Tier II
12 NC_000010.11 10 21078106 + NEBL NNNGGAAATGCATTCTATGG NGG 2 0.1607 Tier II
13 NC_000004.12 4 113175919 + ANK2 NNNGGAAAGGCATTCTGCTG NGG 2 0.1092 Tier II
14 NC_000003.12 3 138735749 - PIK3CB NNNGGAAATTCATTCTTCTG NGG 2 0.1083 Tier II
15 NC_000003.12 3 124193158 - KALRN NNNGGAAATGCAGTCTACAG NGG 2 0.0745 Tier II
16 NC_000002.12 2 225685061 + NYAP2 NNNGGAAATGCATTCTAGTG NAG 2 0.0346 Tier II
17 NC_000020.11 20 46078468 + NCOA5 NNNGGAAATGCAGTTTACTG NGG 2 0.0174 Tier II
18 NC_000015.10 15 68936586 + SPESP1 NNNGGAAATGGATTTTACTG NGG 2 0.0167 Tier II
19 NC_000015.10 15 68936586 + SPESP1-NOX5 NNNGGAAATGGATTTTACTG NGG 2 0.0167 Tier II
20 NC_000016.10 16 20848136 - REXO5 NNNGGAAATGCATGCTACTG NTG 2 0.0 Tier II
21 NC_000012.12 12 111710999 + ACAD10 NNNGGAAATGCATTCTACTG NTC 2 0.0 Tier II
22 NC_000007.14 7 4985314 + RNF216P1 NNNGGTAATACATTCTACTG NGG 2 0.6667 Tier III
23 NC_000003.12 3 151079295 + CLRN1-AS1 NNNGGAGATGAATTCTACTG NGG 2 0.5294 Tier III
24 NC_000015.10 15 24902289 + SNHG14 NNNGGCTATGCATTCTACTG NGG 2 0.1989 Tier III
25 NC_000002.12 2 194890440 - LOC105376755 NNNGGAAATGCAATCTACTG NAG 2 0.1795 Tier III
26 NC_000006.12 6 134823737 + LOC101928277 NNNGGAAATTCATTCTGCTG NGG 2 0.1434 Tier III
27 NC_000015.10 15 47889018 + LOC124900354 NNNGGAAATGCATTCTACTG NTG 1 0.039 Tier III
28 NC_000002.12 2 225685061 + LOC105373914 NNNGGAAATGCATTCTAGTG NAG 2 0.0346 Tier III
29 NC_000007.14 7 116594504 - COMETT NNNGGAATTGCATTTTACTG NGG 2 0.0286 Tier III
30 NC_000002.12 2 13110310 - LOC105373436 NNNGGAAATGAATTCTACTG NGC 2 0.0167 Tier III
31 NC_000002.12 2 13110310 - LOC105373484 NNNGGAAATGAATTCTACTG NGC 2 0.0167 Tier III
32 NC_000006.12 6 106730082 + LINC02532 NNNAGAAATGCATTCTACTG NGT 2 0.0145 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 83374362 + Eml4 NNNAGAAATGCATTCTACTG NAG 2 0.2333 Tier II
2 NC_000086.7 X 75003957 - Gab3 NNNGCAAATGCATTCTACAG NGG 2 0.2245 Tier II
3 NC_000079.6 13 78140828 + Fam172a NNNGGAAATGCATTCTAAAG NGG 2 0.1538 Tier II
4 NC_000084.6 18 24474556 + 2700062C07Rik NNNGGCAATGCATCCTACTG NGG 2 0.1299 Tier II
5 NC_000080.6 14 39039122 - Nrg3 NNNGGAATTGCATTCTACTG NAG 2 0.1111 Tier II
6 NC_000068.7 2 17462632 + Nebl NNNGGAAATGCTTTCTACGG NGG 2 0.0833 Tier II
7 NC_000081.6 15 34413158 - Matn2 NNNGGAAAGGCATTCTAGTG NGG 2 0.0825 Tier II
8 NC_000073.6 7 92088420 + Dlg2 NNNGCAAATGCATTTTACTG NGG 2 0.0524 Tier II
9 NC_000075.6 9 70832704 - Lipc NNNGGAAATGCCTTCTCCTG NGG 2 0.0464 Tier II
10 NC_000082.6 16 73034854 - Robo1 NNNGGAAATGTATTCTTCTG NGG 2 0.041 Tier II
11 NC_000086.7 X 68771597 - Fmr1nb NNNGGAAATGCATTCTAATG NGA 2 0.0374 Tier II
12 NC_000070.6 4 151348865 + Camta1 NNNGGAATTGCATTCTACTG NGA 2 0.0298 Tier II
13 NC_000075.6 9 13778868 - Mtmr2 NNNGGAAATGTATTGTACTG NGG 2 0.0154 Tier II
14 NC_000084.6 18 13879332 + Zfp521 NNNGGAAATGCATGCTACCG NGG 2 0.0 Tier II
15 NC_000073.6 7 107732482 - Ppfibp2 NNNGGAAATGCATGCTTCTG NGG 2 0.0 Tier II
16 NC_000079.6 13 78140828 + Gm38604 NNNGGAAATGCATTCTAAAG NGG 2 0.1538 Tier III
17 NC_000069.6 3 65464482 - Gm34576 NNNTGAAATGTATTCTACTG NGG 2 0.1119 Tier III
18 NC_000078.6 12 115160570 - Igh NNNGAAAATGCATTCTACTG NTG 2 0.0338 Tier III
19 NC_000078.6 12 115210251 - Igh NNNGAAAATGCATTCTACTG NTG 2 0.0338 Tier III
20 NC_000078.6 12 115463163 - Igh NNNGAAAATGCATTCTACTG NTG 2 0.0338 Tier III
21 NC_000078.6 12 115760171 - Igh NNNGAAAATGCATTCTACTG NTG 2 0.0338 Tier III
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Other clones with same target sequence:

(none)