Construct: sgRNA BRDN0001145021
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGGAGCAGAGATACCCCTG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- MINK1 (50488)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000017.11 | 17 | 4893588 | + | MINK1 | NNNGAGCAGAGATACCCCTG | NGG | 0 | 1.0 | Tier I |
2 | NC_000023.11 | X | 86309458 | - | DACH2 | NNNGAGTAGAGATACCCCTG | NGG | 1 | 0.8125 | Tier II |
3 | NC_000011.10 | 11 | 44923841 | - | TSPAN18 | NNNGAACAGAGACACCCCTG | NGG | 2 | 0.7895 | Tier II |
4 | NC_000006.12 | 6 | 144063713 | - | PLAGL1 | NNNGGACAGAGATACCCCTG | NGG | 2 | 0.72 | Tier II |
5 | NC_000014.9 | 14 | 59561982 | + | CCDC175 | NNNGATCAGAGATACACCTG | NGG | 2 | 0.6667 | Tier II |
6 | NC_000012.12 | 12 | 70311463 | - | CNOT2 | NNNGAGGAGAGATACACCTG | NGG | 2 | 0.4706 | Tier II |
7 | NC_000023.11 | X | 152117443 | + | LOC100533997 | NNNGAGCAGAGACTCCCCTG | NGG | 2 | 0.4211 | Tier II |
8 | NC_000007.14 | 7 | 151850961 | - | PRKAG2 | NNNGAGCAGGGGTACCCCTG | NGG | 2 | 0.4012 | Tier II |
9 | NC_000023.11 | X | 72006064 | - | NHSL2 | NNNGAGAAGAGATACCACTG | NGG | 2 | 0.35 | Tier II |
10 | NC_000018.10 | 18 | 36453573 | + | FHOD3 | NNNGAGCAGTTATACCCCTG | NGG | 2 | 0.3394 | Tier II |
11 | NC_000010.11 | 10 | 71999805 | + | CHST3 | NNNGAGCAGAAAGACCCCTG | NGG | 2 | 0.2609 | Tier II |
12 | NC_000001.11 | 1 | 37756434 | - | EPHA10 | NNNCAGCAGAGATACCACTG | NGG | 2 | 0.2471 | Tier II |
13 | NC_000022.11 | 22 | 29721345 | + | CABP7 | NNNGAGCAGTGAGACCCCTG | NGG | 2 | 0.2302 | Tier II |
14 | NC_000016.10 | 16 | 79196462 | - | WWOX | NNNGAGCAGAGATAACCCTG | NGG | 1 | 0.2222 | Tier II |
15 | NC_000011.10 | 11 | 11484648 | - | GALNT18 | NNNCAGCAGAGAGACCCCTG | NGG | 2 | 0.1381 | Tier II |
16 | NC_000009.12 | 9 | 135789715 | + | KCNT1 | NNNGAGCAGAGCTACCACTG | NGG | 2 | 0.1228 | Tier II |
17 | NC_000012.12 | 12 | 5634160 | + | ANO2 | NNNGAGCAGAGCTGCCCCTG | NGG | 2 | 0.1228 | Tier II |
18 | NC_000011.10 | 11 | 27378398 | + | LGR4 | NNNGAGGAGAGATACCCCTG | NAG | 2 | 0.122 | Tier II |
19 | NC_000016.10 | 16 | 87702652 | - | KLHDC4 | NNNGAGCAGAGAGGCCCCTG | NGG | 2 | 0.1217 | Tier II |
20 | NC_000006.12 | 6 | 43010389 | + | PPP2R5D | NNNTAGCAGAGATACCCCTG | NGA | 2 | 0.0253 | Tier II |
21 | NC_000002.12 | 2 | 77174432 | - | LRRTM4 | NNNGAGCAGAGATACACCTG | NGC | 2 | 0.0222 | Tier II |
22 | NC_000019.10 | 19 | 40670500 | + | NUMBL | NNNGAGCAGAGATACACCTG | NGC | 2 | 0.0222 | Tier II |
23 | NC_000002.12 | 2 | 240774310 | - | KIF1A | NNNGAGCAGACATAGCCCTG | NGG | 2 | 0.0214 | Tier II |
24 | NC_000013.11 | 13 | 56696125 | - | LOC105370214 | NNNGATCAGAGACACCCCTG | NGG | 2 | 0.5263 | Tier III |
25 | NC_000023.11 | X | 152117443 | + | MAGEA5P | NNNGAGCAGAGACTCCCCTG | NGG | 2 | 0.4211 | Tier III |
26 | NC_000023.11 | X | 152117443 | + | LOC105373369 | NNNGAGCAGAGACTCCCCTG | NGG | 2 | 0.4211 | Tier III |
27 | NC_000002.12 | 2 | 87471535 | + | CYTOR | NNNGAGCAGAGGTGCCCCTG | NGG | 2 | 0.337 | Tier III |
28 | NC_000002.12 | 2 | 87471535 | + | NCAL1 | NNNGAGCAGAGGTGCCCCTG | NGG | 2 | 0.337 | Tier III |
29 | NC_000002.12 | 2 | 111478676 | - | MIR4435-2HG | NNNGAGCAGAGGTGCCCCTG | NGG | 2 | 0.337 | Tier III |
30 | NC_000005.10 | 5 | 62105269 | - | LOC124900610 | NNNGAGCTGATATACCCCTG | NGG | 2 | 0.1648 | Tier III |
31 | NC_000015.10 | 15 | 79832771 | + | LOC124903536 | NNNGAGCAGAGATACCCTGG | NGG | 2 | 0.1607 | Tier III |
32 | NC_000007.14 | 7 | 99992779 | + | LOC100128334 | NNNCAGCAGAGATACCCCAG | NGG | 2 | 0.1513 | Tier III |
33 | NC_000009.12 | 9 | 135789715 | + | LOC107987140 | NNNGAGCAGAGCTACCACTG | NGG | 2 | 0.1228 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000067.6 | 1 | 75421961 | - | Speg | NNNCAGCAGAGCTACCCCTG | NGG | 2 | 0.1393 | Tier I |
2 | NC_000081.6 | 15 | 32459390 | - | Sema5a | NNNGAGAAGAGAAACCCCTG | NGG | 2 | 0.5192 | Tier II |
3 | NC_000083.6 | 17 | 65728104 | - | Rab31 | NNNGACCAGAGGTACCCCTG | NGG | 2 | 0.4924 | Tier II |
4 | NC_000073.6 | 7 | 135095717 | - | Dock1 | NNNGAGCTGTGATACCCCTG | NGG | 2 | 0.3782 | Tier II |
5 | NC_000070.6 | 4 | 123107064 | - | Bmp8b | NNNGATCAGAGATTCCCCTG | NGG | 2 | 0.3556 | Tier II |
6 | NC_000068.7 | 2 | 153495233 | - | Nol4l | NNNGAGAAGAGATGCCCCTG | NGG | 2 | 0.35 | Tier II |
7 | NC_000073.6 | 7 | 29941002 | + | Zfp74 | NNNGACCAGAGATACCACTG | NGG | 2 | 0.3182 | Tier II |
8 | NC_000068.7 | 2 | 31429149 | + | Hmcn2 | NNNGAGCAGAGATGCCTCTG | NGG | 2 | 0.2178 | Tier II |
9 | NC_000073.6 | 7 | 66815305 | + | Cers3 | NNNGGGCAGAGCTACCCCTG | NGG | 2 | 0.1895 | Tier II |
10 | NC_000081.6 | 15 | 96404001 | - | Arid2 | NNNGAGCACAGAGACCCCTG | NGG | 2 | 0.1405 | Tier II |
11 | NC_000071.6 | 5 | 148388259 | + | Slc7a1 | NNNGAGCAGCGAGACCCCTG | NGG | 2 | 0.087 | Tier II |
12 | NC_000068.7 | 2 | 25336618 | - | Man1b1 | NNNGAGCAGAGACACCCCTG | NGA | 2 | 0.0548 | Tier II |
13 | NC_000085.6 | 19 | 10930476 | + | Ptgdr2 | NNNGAGCAGAGATGTCCCTG | NGG | 2 | 0.0311 | Tier II |
14 | NC_000073.6 | 7 | 98071225 | + | Myo7a | NNNGAGCACAGATAGCCCTG | NGG | 2 | 0.0269 | Tier II |
15 | NC_000082.6 | 16 | 31012656 | - | Xxylt1 | NNNGAGCAGAGATACCCCAG | NGA | 2 | 0.0198 | Tier II |
16 | NC_000074.6 | 8 | 119762882 | + | Meak7 | NNNGAGCAGAGAGACCCCTG | NGA | 2 | 0.0181 | Tier II |
17 | NC_000080.6 | 14 | 9869528 | + | Fhit | NNNGAGCAGAGCTACCCCTG | NGC | 2 | 0.0058 | Tier II |
18 | NC_000068.7 | 2 | 153495233 | - | 4930404H24Rik | NNNGAGAAGAGATGCCCCTG | NGG | 2 | 0.35 | Tier III |
19 | NC_000079.6 | 13 | 107128596 | + | Gm31452 | NNNGAGCAGAGATTCCCCTG | NAG | 2 | 0.1383 | Tier III |
20 | NC_000079.6 | 13 | 107128596 | + | Gm41051 | NNNGAGCAGAGATTCCCCTG | NAG | 2 | 0.1383 | Tier III |
21 | NC_000085.6 | 19 | 21105113 | - | 4930554I06Rik | NNNGAGCAGATTTACCCCTG | NGG | 2 | 0.1282 | Tier III |
22 | NC_000079.6 | 13 | 71740988 | - | Gm40999 | NNNGAGCTGAGATAACCCTG | NGG | 2 | 0.0952 | Tier III |