Construct: sgRNA BRDN0001145025
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TACCTTGCAAATAATCTGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ULK2 (9706)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77261
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
19849383 |
+ |
ULK2 |
NNNCTTGCAAATAATCTGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
90350274 |
- |
CCSER1 |
NNNCTCGCAAATAATCTGCT |
NGG |
2 |
0.6364 |
Tier II |
| 3 |
NC_000013.11 |
13 |
48513692 |
- |
RCBTB2 |
NNNCTTGAAAATAATCTGCA |
NGG |
2 |
0.6094 |
Tier II |
| 4 |
NC_000004.12 |
4 |
71400048 |
- |
SLC4A4 |
NNNCTTGCAAATAATCAGCA |
NGG |
2 |
0.5 |
Tier II |
| 5 |
NC_000017.11 |
17 |
37051389 |
- |
AATF |
NNNCCTGCAAATAATCTGTG |
NGG |
2 |
0.4615 |
Tier II |
| 6 |
NC_000013.11 |
13 |
76882369 |
+ |
KCTD12 |
NNNCTTTCAAATAATGTGCG |
NGG |
2 |
0.0879 |
Tier II |
| 7 |
NC_000017.11 |
17 |
37051389 |
- |
LOC105371753 |
NNNCCTGCAAATAATCTGTG |
NGG |
2 |
0.4615 |
Tier III |
| 8 |
NC_000007.14 |
7 |
11958266 |
- |
LOC124901589 |
NNNCTTGCAAATAATCTAAG |
NGG |
2 |
0.2967 |
Tier III |
| 9 |
NC_000001.11 |
1 |
70744580 |
- |
LINC01788 |
NNNCTTGAAAATCATCTGCG |
NGG |
2 |
0.1368 |
Tier III |
| 10 |
NC_000009.12 |
9 |
78369408 |
+ |
LOC107987083 |
NNNCTTGCAAATAATCTGCA |
NTG |
2 |
0.0365 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
61840124 |
+ |
Ulk2 |
NNNCTTGCAAATAATCTGCC |
NGG |
1 |
0.4286 |
Tier I |
| 2 |
NC_000071.6 |
5 |
48962986 |
+ |
Kcnip4 |
NNNCTAGCAAATAATCTGCA |
NGG |
2 |
0.8125 |
Tier II |
| 3 |
NC_000077.6 |
11 |
79113246 |
+ |
Ksr1 |
NNNCTTGCACAAAATCTGCG |
NGG |
2 |
0.2667 |
Tier II |
| 4 |
NC_000076.6 |
10 |
105435086 |
+ |
Tmtc2 |
NNNCTTGCAAATAATCTGCC |
NGA |
2 |
0.0298 |
Tier II |
| 5 |
NC_000072.6 |
6 |
6655923 |
- |
Gm20618 |
NNNCTTGCAAGTAAGCTGCG |
NGG |
2 |
0.0325 |
Tier III |
Other clones with same target sequence:
(none)
