Construct: sgRNA BRDN0001145028
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCAGAGAATTCGGTCAACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP2K4 (6416)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76650
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
12095588 |
+ |
MAP2K4 |
NNNGAGAATTCGGTCAACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
11134115 |
+ |
TAS2R30 |
NNNGAGAATTTGGTCAACAA |
NGG |
2 |
0.2885 |
Tier I |
| 3 |
NC_000023.11 |
X |
152288749 |
- |
GABRA3 |
NNNAAGAATTCTGTCAACAG |
NGG |
2 |
0.3462 |
Tier II |
| 4 |
NC_000013.11 |
13 |
113480007 |
- |
DCUN1D2 |
NNNGACATTTCGGTCAACAG |
NGG |
2 |
0.2922 |
Tier II |
| 5 |
NC_000015.10 |
15 |
67172189 |
- |
SMAD3 |
NNNGAGAATTATGTCAACAG |
NGG |
2 |
0.2885 |
Tier II |
| 6 |
NC_000012.12 |
12 |
11134115 |
+ |
PRH1 |
NNNGAGAATTTGGTCAACAA |
NGG |
2 |
0.2885 |
Tier II |
| 7 |
NC_000012.12 |
12 |
11134115 |
+ |
PRH1-TAS2R14 |
NNNGAGAATTTGGTCAACAA |
NGG |
2 |
0.2885 |
Tier II |
| 8 |
NC_000003.12 |
3 |
119704734 |
+ |
CFAP91 |
NNNGATATTTCGGTCAACAG |
NGG |
2 |
0.2857 |
Tier II |
| 9 |
NC_000008.11 |
8 |
143076298 |
- |
LY6S |
NNNGAGAATTGGGTCAGCAG |
NGG |
2 |
0.0441 |
Tier II |
| 10 |
NC_000008.11 |
8 |
102099048 |
+ |
NCALD |
NNNGAGAATTCGGTCAACAC |
NGA |
2 |
0.0298 |
Tier II |
| 11 |
NC_000010.11 |
10 |
10888352 |
- |
CELF2 |
NNNGAGAATTCAGTCTACAG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000012.12 |
12 |
11134115 |
+ |
PRH1-PRR4 |
NNNGAGAATTTGGTCAACAA |
NGG |
2 |
0.2885 |
Tier III |
| 13 |
NC_000023.11 |
X |
73525750 |
- |
MAP2K4P1 |
NNNAAGAATTCGGTCAACAG |
NGA |
2 |
0.0625 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
65719591 |
- |
Map2k4 |
NNNGAGAATTCGGTCAACTG |
NGG |
1 |
0.5385 |
Tier I |
| 2 |
NC_000071.6 |
5 |
24383672 |
+ |
Nos3 |
NNNGAGAATTCTGGCAACAG |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000075.6 |
9 |
104201979 |
+ |
Dnajc13 |
NNNGACAATTCAGTCAACAG |
NGG |
2 |
0.6364 |
Tier II |
| 4 |
NC_000077.6 |
11 |
52100020 |
- |
Ppp2ca |
NNNGAGAAATCGGTCAACAC |
NGG |
2 |
0.398 |
Tier II |
| 5 |
NC_000072.6 |
6 |
83945608 |
- |
Zfp638 |
NNNGAAAATTCTGTCAACAG |
NGG |
2 |
0.3846 |
Tier II |
| 6 |
NC_000071.6 |
5 |
43608886 |
+ |
C1qtnf7 |
NNNGAGGATTCTGTCAACAG |
NGG |
2 |
0.2715 |
Tier II |
| 7 |
NC_000073.6 |
7 |
68739652 |
- |
Arrdc4 |
NNNGAGAATTCCGTCACCAG |
NGG |
2 |
0.0934 |
Tier II |
| 8 |
NC_000084.6 |
18 |
54936075 |
+ |
Zfp608 |
NNNGAGAATTGGGTCAACAG |
NGT |
2 |
0.004 |
Tier II |
| 9 |
NC_000075.6 |
9 |
104201979 |
+ |
Gm46130 |
NNNGACAATTCAGTCAACAG |
NGG |
2 |
0.6364 |
Tier III |
Other clones with same target sequence:
(none)
