Construct: sgRNA BRDN0001145029

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACGGCATATGAACTGCTGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK32A (202374)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76474

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 147361605 + STK32A NNNGCATATGAACTGCTGAG NGG 0 1.0 Tier I
2 NC_000009.12 9 69447927 - APBA1 NNNGCATGAGAACTGCTGAG NGG 2 0.681 Tier II
3 NC_000012.12 12 11789405 - ETV6 NNNGCATCTGAACTACTGAG NGG 2 0.4034 Tier II
4 NC_000017.11 17 81551326 - FAAP100 NNNGCAGATGGACTGCTGAG NGG 2 0.3824 Tier II
5 NC_000020.11 20 20177797 + CFAP61 NNNGGATAGGAACTGCTGAG NGG 2 0.3714 Tier II
6 NC_000010.11 10 131215367 + TCERG1L NNNACATATCAACTGCTGAG NGG 2 0.36 Tier II
7 NC_000003.12 3 52922870 + SFMBT1 NNNGCCTATGGACTGCTGAG NGG 2 0.2955 Tier II
8 NC_000006.12 6 70828541 - SMAP1 NNNGCATATGAACTGCAGTG NGG 2 0.2872 Tier II
9 NC_000006.12 6 72064451 + RIMS1 NNNGCATATGAATTGTTGAG NGG 2 0.2154 Tier II
10 NC_000004.12 4 152519962 + FBXW7 NNNTCATATGAACTGCTGTG NGG 2 0.1958 Tier II
11 NC_000006.12 6 157184095 + ARID1B NNNGCCTATGCACTGCTGAG NGG 2 0.1818 Tier II
12 NC_000005.10 5 116091706 - COMMD10 NNNGCATATGAACTGCTGAA NGA 2 0.0651 Tier II
13 NC_000014.9 14 68505911 + RAD51B NNNGCATATGTACTGCTGAG NGA 2 0.0214 Tier II
14 NC_000013.11 13 91492637 - GPC5 NNNGCATATGAACTGGTGAG NTG 2 0.006 Tier II
15 NC_000010.11 10 95169213 - LOC107984257 NNNGTATATGAATTGCTGAG NGG 2 0.4455 Tier III
16 NC_000003.12 3 129321540 - H1-10-AS1 NNNGCATGTGAACTGGTGAG NGG 2 0.1128 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 43305088 + Stk32a NNNGCTTACGAACTGCTGAG NGG 2 0.6593 Tier I
2 NC_000079.6 13 57744394 - Spock1 NNNACATGTGAACTGCTGAG NGG 2 0.66 Tier II
3 NC_000072.6 6 72672827 - Tcf7l1 NNNGCATAGGAACTGCTGAG NGG 1 0.619 Tier II
4 NC_000071.6 5 38281214 + Otop1 NNNGCATATTAACTGCTTAG NGG 2 0.5417 Tier II
5 NC_000078.6 12 110637949 + Dync1h1 NNNGCAGATGAGCTGCTGAG NGG 2 0.4248 Tier II
6 NC_000076.6 10 73463084 - Pcdh15 NNNGGACATGAACTGCTGAG NGG 2 0.4125 Tier II
7 NC_000069.6 3 82114922 - Gucy1a1 NNNGCAGAGGAACTGCTGAG NGG 2 0.3641 Tier II
8 NC_000079.6 13 33006760 + Serpinb9 NNNGCATAAGAACTGCTGAG NAG 2 0.2407 Tier II
9 NC_000069.6 3 36643265 + Trpc3 NNNTCATATGAACTGCTGTG NGG 2 0.1958 Tier II
10 NC_000068.7 2 101575794 - Iftap NNNGCATATGAACTGCTCAG NAG 2 0.1235 Tier II
11 NC_000072.6 6 89673479 + Txnrd3 NNNGCATATGGAGTGCTGAG NGG 2 0.0886 Tier II
12 NC_000079.6 13 43083190 - Phactr1 NNNGCATATGAACTACTGAG NGA 2 0.0654 Tier II
13 NC_000080.6 14 14983058 - Nek10 NNNGCATAAGAACTGCTGAG NGA 2 0.0645 Tier II
14 NC_000073.6 7 19880555 + Ceacam19 NNNGCATAAGAACTGCTGAG NTG 2 0.0362 Tier II
15 NC_000081.6 15 81954885 + Pmm1 NNNGCATTTGAACTGCTGAG NGA 2 0.0298 Tier II
16 NC_000070.6 4 18878938 - Cnbd1 NNNGCATATGAACTGTTGAG NGA 2 0.0214 Tier II
17 NC_000072.6 6 72672827 - Gm31391 NNNGCATAGGAACTGCTGAG NGG 1 0.619 Tier III
18 NC_000079.6 13 16164018 + Gm31711 NNNGCATATGAACTGGTCAG NGG 2 0.0733 Tier III
19 NC_000077.6 11 75268740 - Gm35117 NNNGCATATGAGCTGCTGAG NGA 2 0.0502 Tier III
20 NC_000083.6 17 19840162 - Vmn2r-ps123 NNNGCACATGAACTGCTGAG NGT 2 0.0111 Tier III
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Other clones with same target sequence:

(none)