Construct: sgRNA BRDN0001145030
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTGCACCTCGAAGAGCACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NRBP2 (340371)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76455
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
143837682 |
+ |
NRBP2 |
NNNGCACCTCGAAGAGCACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
103110166 |
- |
EXOC3L4 |
NNNTCTCCTCGAAGAGCACG |
NGG |
2 |
0.2597 |
Tier I |
| 3 |
NC_000010.11 |
10 |
399137 |
+ |
DIP2C |
NNNGCACCTCGATGGGCACG |
NGG |
2 |
0.195 |
Tier I |
| 4 |
NC_000020.11 |
20 |
59939174 |
+ |
PPP1R3D |
NNNGCACCGCGAAGTGCACG |
NGG |
2 |
0.1238 |
Tier I |
| 5 |
NC_000009.12 |
9 |
132198385 |
- |
NTNG2 |
NNNGCACCTCGAAGCGCACG |
NGC |
2 |
0.0061 |
Tier I |
| 6 |
NC_000015.10 |
15 |
80509116 |
- |
ARNT2 |
NNNGCACCTTGAAGAGCACG |
NGG |
1 |
0.9412 |
Tier II |
| 7 |
NC_000019.10 |
19 |
45998088 |
+ |
CCDC61 |
NNNGCACCTAGAAGAGCACA |
NGG |
2 |
0.8125 |
Tier II |
| 8 |
NC_000005.10 |
5 |
1080672 |
+ |
SLC12A7 |
NNNGCGACTCGAAGAGCACG |
NGG |
2 |
0.5357 |
Tier II |
| 9 |
NC_000020.11 |
20 |
59939174 |
+ |
FAM217B |
NNNGCACCGCGAAGTGCACG |
NGG |
2 |
0.1238 |
Tier II |
| 10 |
NC_000015.10 |
15 |
28944904 |
+ |
APBA2 |
NNNGCACCTGGAAGAGCAGG |
NGG |
2 |
0.0486 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
76088924 |
+ |
Nrbp2 |
NNNGCACCTCAAAGAGCACT |
NGG |
2 |
0.7 |
Tier I |
| 2 |
NC_000078.6 |
12 |
111430795 |
- |
Exoc3l4 |
NNNTCTCCTCGAAGAGCACG |
NGG |
2 |
0.2597 |
Tier I |
| 3 |
NC_000070.6 |
4 |
59189694 |
- |
Ugcg |
NNNGCACCACGAAGAGCACG |
NAG |
2 |
0.2407 |
Tier I |
| 4 |
NC_000081.6 |
15 |
72590773 |
+ |
Trappc9 |
NNNGCACCTCCAAGCGCACG |
NGG |
2 |
0.1169 |
Tier I |
| 5 |
NC_000070.6 |
4 |
91290104 |
+ |
Elavl2 |
NNNGCACCTCGAAGGGGACG |
NGG |
2 |
0.0382 |
Tier II |
| 6 |
NC_000077.6 |
11 |
30705663 |
+ |
Gm12099 |
NNNGCACCTCCAAAAGCACG |
NGG |
2 |
0.3214 |
Tier III |
Other clones with same target sequence:
(none)
