Construct: sgRNA BRDN0001145031
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTACAGGAGTACAAACCACC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DYRK1A (1859)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76755
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000021.9 |
21 |
37505294 |
+ |
DYRK1A |
NNNCAGGAGTACAAACCACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
16532604 |
+ |
KIF16B |
NNNCAAGAGAACAAACCACC |
NGG |
2 |
0.8571 |
Tier II |
| 3 |
NC_000010.11 |
10 |
35072043 |
- |
CUL2 |
NNNCAGGAGTGCAAACCACA |
NGG |
2 |
0.325 |
Tier II |
| 4 |
NC_000001.11 |
1 |
66242935 |
+ |
PDE4B |
NNNCAGGAATAGAAACCACC |
NGG |
2 |
0.2857 |
Tier II |
| 5 |
NC_000001.11 |
1 |
224734729 |
- |
CNIH3 |
NNNCAGGAGTCAAAACCACC |
NGG |
2 |
0.2857 |
Tier II |
| 6 |
NC_000023.11 |
X |
2271599 |
+ |
DHRSX |
NNNCAGGAGTATAAACCAAC |
NGG |
2 |
0.2308 |
Tier II |
| 7 |
NC_000024.10 |
Y |
2271599 |
+ |
DHRSX |
NNNCAGGAGTATAAACCAAC |
NGG |
2 |
0.2308 |
Tier II |
| 8 |
NC_000008.11 |
8 |
103732622 |
- |
RIMS2 |
NNNCAGTAGTACAAAGCACC |
NGG |
2 |
0.0879 |
Tier II |
| 9 |
NC_000014.9 |
14 |
22612292 |
- |
ABHD4 |
NNNCAGTAGTACAAAGCACC |
NGG |
2 |
0.0879 |
Tier II |
| 10 |
NC_000001.11 |
1 |
23334588 |
- |
HNRNPR |
NNNCAGGAGTACAAACCAGA |
NGG |
2 |
0.0625 |
Tier II |
| 11 |
NC_000010.11 |
10 |
121505045 |
- |
FGFR2 |
NNNCAGGAGTACTATCCACC |
NGG |
2 |
0.06 |
Tier II |
| 12 |
NC_000016.10 |
16 |
10535937 |
+ |
EMP2 |
NNNCAGGAGTACAAATCCCC |
NGG |
2 |
0.0586 |
Tier II |
| 13 |
NC_000022.11 |
22 |
28924548 |
+ |
ZNRF3 |
NNNCAGGAGTTCAAACCAGC |
NGG |
2 |
0.0385 |
Tier II |
| 14 |
NC_000006.12 |
6 |
96506385 |
- |
UFL1-AS1 |
NNNCAGGAGAACCAACCACC |
NGG |
2 |
0.1805 |
Tier III |
| 15 |
NC_000007.14 |
7 |
72831794 |
+ |
SBDSP1 |
NNNCAGGAGTACAAAACAGC |
NGG |
2 |
0.125 |
Tier III |
| 16 |
NC_000002.12 |
2 |
122183470 |
- |
LOC105373592 |
NNNCAGGAGCACACACCACC |
NGG |
2 |
0.1143 |
Tier III |
| 17 |
NC_000008.11 |
8 |
2821756 |
- |
LOC105377785 |
NNNCAGGAGTTCAAACCACC |
NTG |
2 |
0.012 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
94685046 |
+ |
Dyrk1a |
NNNTAGGAGTACAAACCACC |
NGG |
1 |
0.8 |
Tier I |
| 2 |
NC_000080.6 |
14 |
39455614 |
+ |
Nrg3 |
NNNCAGGAATAAAAACCACC |
NGG |
2 |
0.4592 |
Tier II |
| 3 |
NC_000080.6 |
14 |
117309365 |
- |
Gpc6 |
NNNCAGCAGCACAAACCACC |
NGG |
2 |
0.3667 |
Tier II |
| 4 |
NC_000078.6 |
12 |
36160072 |
- |
Ankmy2 |
NNNCAGAAGTTCAAACCACC |
NGG |
2 |
0.3077 |
Tier II |
| 5 |
NC_000079.6 |
13 |
51505196 |
+ |
Shc3 |
NNNCAGGATCACAAACCACC |
NGG |
2 |
0.2844 |
Tier II |
| 6 |
NC_000085.6 |
19 |
6956535 |
- |
Plcb3 |
NNNGAGGAGGACAAACCACC |
NGG |
2 |
0.25 |
Tier II |
| 7 |
NC_000075.6 |
9 |
70381734 |
+ |
Myo1e |
NNNCAGGATTACAAACCAAC |
NGG |
2 |
0.2286 |
Tier II |
| 8 |
NC_000077.6 |
11 |
79082479 |
- |
Ksr1 |
NNNCAGAAGTACAAAGCACC |
NGG |
2 |
0.1538 |
Tier II |
| 9 |
NC_000072.6 |
6 |
118735681 |
- |
Cacna1c |
NNNCAGGAGTTCAAACCTCC |
NGG |
2 |
0.1538 |
Tier II |
| 10 |
NC_000083.6 |
17 |
72506169 |
- |
Alk |
NNNCAGGAGTAAAAACCCCC |
NGG |
2 |
0.1361 |
Tier II |
| 11 |
NC_000076.6 |
10 |
74009068 |
+ |
Pcdh15 |
NNNCAGGAGTACAAACCACA |
NAG |
2 |
0.1296 |
Tier II |
| 12 |
NC_000080.6 |
14 |
54269097 |
- |
Abhd4 |
NNNCAGTAGTACAAAGCACC |
NGG |
2 |
0.0879 |
Tier II |
| 13 |
NC_000071.6 |
5 |
48600303 |
+ |
Kcnip4 |
NNNCAGGATTACAAAGCACC |
NGG |
2 |
0.0821 |
Tier II |
| 14 |
NC_000083.6 |
17 |
66562392 |
- |
Themis3 |
NNNCAGGAGAACAAACCACC |
NGA |
2 |
0.0595 |
Tier II |
| 15 |
NC_000077.6 |
11 |
120008998 |
+ |
Aatk |
NNNCAGGAGTACAGACCACC |
NTG |
2 |
0.0182 |
Tier II |
| 16 |
NC_000072.6 |
6 |
36979830 |
+ |
Dgki |
NNNCAGGAGTACCAACCACC |
NGC |
2 |
0.0047 |
Tier II |
| 17 |
NC_000081.6 |
15 |
69960698 |
+ |
Gm19782 |
NNNCTGGAGAACAAACCACC |
NGG |
2 |
0.3117 |
Tier III |
| 18 |
NC_000083.6 |
17 |
14234530 |
+ |
Gm41531 |
NNNCAGGTATACAAACCACC |
NGG |
2 |
0.2755 |
Tier III |
Other clones with same target sequence:
(none)
