Construct: sgRNA BRDN0001145035
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTGACTATTTGGTTGCACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MARK3 (4140)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77716
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
103457167 |
+ |
MARK3 |
NNNGACTATTTGGTTGCACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
102838031 |
+ |
PAM |
NNNGAATATTTAGTTGCACA |
NGG |
2 |
0.8667 |
Tier II |
| 3 |
NC_000005.10 |
5 |
83314333 |
+ |
XRCC4 |
NNNAACCATTTGGTTGCACA |
NGG |
2 |
0.6188 |
Tier II |
| 4 |
NC_000002.12 |
2 |
100096692 |
+ |
AFF3 |
NNNGATTATTTGGATGCACA |
NGG |
2 |
0.5748 |
Tier II |
| 5 |
NC_000002.12 |
2 |
109768495 |
- |
RANBP2 |
NNNGATTATTTGGATGCACA |
NGG |
2 |
0.5748 |
Tier II |
| 6 |
NC_000002.12 |
2 |
109768495 |
- |
RGPD5 |
NNNGATTATTTGGATGCACA |
NGG |
2 |
0.5748 |
Tier II |
| 7 |
NC_000002.12 |
2 |
110602994 |
+ |
RGPD6 |
NNNGATTATTTGGATGCACA |
NGG |
2 |
0.5748 |
Tier II |
| 8 |
NC_000007.14 |
7 |
48392866 |
+ |
ABCA13 |
NNNGACTAACTGGTTGCACA |
NGG |
2 |
0.4952 |
Tier II |
| 9 |
NC_000010.11 |
10 |
76907501 |
+ |
KCNMA1 |
NNNGCCTATGTGGTTGCACA |
NGG |
2 |
0.25 |
Tier II |
| 10 |
NC_000004.12 |
4 |
164381225 |
- |
MARCHF1 |
NNNGCCTATTTTGTTGCACA |
NGG |
2 |
0.1923 |
Tier II |
| 11 |
NC_000010.11 |
10 |
1717293 |
+ |
ADARB2 |
NNNGTGTATTTGGTTGCACA |
NGG |
2 |
0.1818 |
Tier II |
| 12 |
NC_000016.10 |
16 |
72196667 |
+ |
PMFBP1 |
NNNGACTATTTGGTTGCATA |
NAG |
2 |
0.1197 |
Tier II |
| 13 |
NC_000011.10 |
11 |
45146282 |
+ |
PRDM11 |
NNNGACTATTTGGTTCCAAA |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000003.12 |
3 |
58143747 |
- |
FLNB |
NNNGACTGTTTGGTTTCACA |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000001.11 |
1 |
41538177 |
+ |
HIVEP3 |
NNNGACTTTTTGGGTGCACA |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000010.11 |
10 |
76907501 |
+ |
KCNMA1-AS1 |
NNNGCCTATGTGGTTGCACA |
NGG |
2 |
0.25 |
Tier III |
| 17 |
NC_000003.12 |
3 |
102606976 |
+ |
LOC105374016 |
NNNGACTATTTGCTTGCTCA |
NGG |
2 |
0.2105 |
Tier III |
| 18 |
NT_187383.1 |
16 |
543552 |
+ |
LOC102723945 |
NNNGACTATTTGGGTGCACA |
NTG |
2 |
0.0 |
Tier III |
| 19 |
NC_000011.10 |
11 |
45146282 |
+ |
LOC105376652 |
NNNGACTATTTGGTTCCAAA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
111623270 |
+ |
Mark3 |
NNNGACTATTTGGTTGCACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000077.6 |
11 |
51628746 |
+ |
Rmnd5b |
NNNGACTAGTTAGTTGCACA |
NGG |
2 |
0.5778 |
Tier II |
| 3 |
NC_000070.6 |
4 |
149917762 |
- |
Spsb1 |
NNNGACAATTTGGTAGCACA |
NGG |
2 |
0.5066 |
Tier II |
| 4 |
NC_000083.6 |
17 |
14851578 |
+ |
Wdr27 |
NNNGACTTTTAGGTTGCACA |
NGG |
2 |
0.3214 |
Tier II |
| 5 |
NC_000072.6 |
6 |
104572313 |
- |
Cntn6 |
NNNGACTATTTTGTTGCACA |
NAG |
2 |
0.0997 |
Tier II |
| 6 |
NC_000075.6 |
9 |
24221190 |
- |
Npsr1 |
NNNGACTAGTTGGTTTCACA |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000079.6 |
13 |
78634351 |
+ |
Gm32067 |
NNNGACTATTAGCTTGCACA |
NGG |
2 |
0.3158 |
Tier III |
| 8 |
NC_000071.6 |
5 |
100749500 |
+ |
Gm36619 |
NNNGAATATTTGGTGGCACA |
NGG |
2 |
0.0464 |
Tier III |
Other clones with same target sequence:
(none)
