Construct: sgRNA BRDN0001145045
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCATGCCATCAACTTGACGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NTRK3 (4916)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76179
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
88136001 |
- |
NTRK3 |
NNNTGCCATCAACTTGACGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
63546047 |
- |
EFCAB7 |
NNNTGCCTTCAACTTGACTC |
NGG |
2 |
0.2857 |
Tier I |
| 3 |
NC_000018.10 |
18 |
11110747 |
- |
PIEZO2 |
NNNTGCCATCAGCTTGACGG |
NGG |
2 |
0.0425 |
Tier II |
| 4 |
NC_000010.11 |
10 |
22682559 |
+ |
PIP4K2A |
NNNTGCCATCAACTTGACAC |
NTG |
2 |
0.0278 |
Tier II |
| 5 |
NC_000007.14 |
7 |
142372914 |
+ |
TRB |
NNNTGCCAGCAACTTGATGC |
NGG |
2 |
0.398 |
Tier III |
| 6 |
NC_000007.14 |
7 |
142372914 |
+ |
TRBV5-2 |
NNNTGCCAGCAACTTGATGC |
NGG |
2 |
0.398 |
Tier III |
| 7 |
NC_000004.12 |
4 |
79018101 |
- |
LINC01088 |
NNNTGCCATCACCTTGACAC |
NGG |
2 |
0.188 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
78461147 |
- |
Ntrk3 |
NNNTGCCATCAACTTGACCC |
NGG |
1 |
0.4483 |
Tier I |
| 2 |
NC_000067.6 |
1 |
126522486 |
+ |
Nckap5 |
NNNTGTCATCAACTTGACTC |
NGG |
2 |
0.619 |
Tier II |
| 3 |
NC_000086.7 |
X |
84893382 |
+ |
Dmd |
NNNTGCCATCCACTTGATGC |
NGG |
2 |
0.2571 |
Tier II |
| 4 |
NC_000076.6 |
10 |
59115683 |
- |
Sh3rf3 |
NNNTGCCAACAACTTCACGC |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000071.6 |
5 |
91434878 |
+ |
Gm42110 |
NNNTGCCATCAACATGAAGC |
NGG |
2 |
0.3333 |
Tier III |
Other clones with same target sequence:
(none)
