Construct: sgRNA BRDN0001145050

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATAGCCAGCCTTAAACATGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RIOK2 (55781)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76960

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 97179123 - RIOK2 NNNGCCAGCCTTAAACATGG NGG 0 1.0 Tier I
2 NC_000002.12 2 71148045 + MPHOSPH10 NNNGCCAGCCATAACCATGG NGG 2 0.2045 Tier I
3 NC_000007.14 7 158413848 + PTPRN2 NNNGCCACCCTTAAACATGG NGG 1 0.6154 Tier II
4 NC_000001.11 1 156835296 + NTRK1 NNNCCCAGCCTAAAACATGG NGG 2 0.4235 Tier II
5 NC_000005.10 5 144355151 - KCTD16 NNNGCAAGCCGTAAACATGG NGG 2 0.3714 Tier II
6 NC_000017.11 17 7493210 - POLR2A NNNGCCTGCCTGAAACATGG NGG 2 0.2188 Tier II
7 NC_000014.9 14 51947502 + GNG2 NNNGCCAGCTTTAAAGATGG NGG 2 0.1448 Tier II
8 NC_000009.12 9 114469919 - WHRN NNNGCCAGCCTTGAAGATGG NGG 2 0.1003 Tier II
9 NC_000023.11 X 7208930 - STS NNNGCCAGCCTTGAAGATGG NGG 2 0.1003 Tier II
10 NC_000001.11 1 26841178 - ZDHHC18 NNNACCAGCCTTAAACATGG NGA 2 0.0625 Tier II
11 NC_000023.11 X 14728628 - FANCB NNNGCCAGCCCTAAACATGG NTG 2 0.026 Tier II
12 NC_000023.11 X 14728628 - GLRA2 NNNGCCAGCCCTAAACATGG NTG 2 0.026 Tier II
13 NC_000004.12 4 16224704 + TAPT1 NNNTCCAGCCTTAAACATGG NGA 2 0.0253 Tier II
14 NC_000009.12 9 68597208 + TMEM252-DT NNNACCAGCCTAAAACATGG NGG 2 0.72 Tier III
15 NC_000004.12 4 130384165 - LINC02479 NNNGTCAGCCTTAAAAATGG NGG 2 0.6364 Tier III
16 NC_000006.12 6 32389510 + TSBP1-AS1 NNNGCGAGCCCTAAACATGG NGG 2 0.3333 Tier III
17 NC_000002.12 2 237030290 + COPS8-DT NNNGCCAGCCTTCAGCATGG NGG 2 0.1368 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 17377790 + Riok2 NNNGCCAGCCTGAAACATGG NGG 1 0.5 Tier I
2 NC_000073.6 7 64378800 - Mphosph10 NNNGCCAGCCATAACCATGG NGG 2 0.2045 Tier I
3 NC_000070.6 4 152300615 - Icmt NNNGCCAGCCGTAAACATGG NAG 2 0.1037 Tier I
4 NC_000079.6 13 92333008 + Msh3 NNNGCCAGTCATAAACATGG NGG 2 0.6563 Tier II
5 NC_000075.6 9 77347456 + Mlip NNNGCCAGCTTTGAACATGG NGG 2 0.6138 Tier II
6 NC_000072.6 6 121035897 - Mical3 NNNGCAAGCCTTGAACATGG NGG 2 0.6056 Tier II
7 NC_000073.6 7 49073625 + Nav2 NNNGCCAGCCTAAAACATAG NGG 2 0.5714 Tier II
8 NC_000072.6 6 35237624 - Nup205 NNNGACAGCCTTAAACATGA NGG 2 0.5357 Tier II
9 NC_000072.6 6 144056889 + Sox5 NNNGCCAGCCTCAGACATGG NGG 2 0.4421 Tier II
10 NC_000068.7 2 59824366 + Tanc1 NNNGGCAGCCTGAAACATGG NGG 2 0.3 Tier II
11 NC_000084.6 18 67969045 + Ldlrad4 NNNGCCAGACTTAAACATGG NAG 2 0.2222 Tier II
12 NC_000071.6 5 105706586 + Lrrc8d NNNGCCAGCCTAAAACATGG NAG 2 0.2074 Tier II
13 NC_000068.7 2 122774852 - Sqor NNNGCCAGCTTTAAAGATGG NGG 2 0.1448 Tier II
14 NC_000076.6 10 21156038 + Myb NNNTCCAGCCTTAAAGATGG NGG 2 0.0559 Tier II
15 NC_000076.6 10 61944200 - Col13a1 NNNGCCAGCCTGAAACATGG NCG 2 0.0536 Tier II
16 NC_000069.6 3 101578497 - Atp1a1 NNNCCCAGCCTTAAACATGG NGA 2 0.0368 Tier II
17 NC_000074.6 8 16981285 + Csmd1 NNNGCCATCCTTAAACATGG NTG 2 0.0244 Tier II
18 NC_000073.6 7 46687094 + Saal1 NNNGCCAGCCTTAAAGATGG NCG 2 0.0165 Tier II
19 NC_000079.6 13 58384804 - 2210016F16Rik NNNGCCAGCCTTAAACCTGG NTG 2 0.0069 Tier II
20 NC_000071.6 5 115015071 - Sppl3 NNNGCCAGCCTTAAACTTGG NGC 2 0.003 Tier II
21 NC_000073.6 7 49073625 + Gm32849 NNNGCCAGCCTAAAACATAG NGG 2 0.5714 Tier III
22 NC_000072.6 6 147652478 - Gm36244 NNNGCCAGCCTTAAACATCG NGG 1 0.4483 Tier III
23 NC_000080.6 14 79910905 - Gm6999 NNNTCCTGCCTTAAACATGG NGG 2 0.1591 Tier III
24 NC_000070.6 4 16257238 - A530072M11Rik NNNGACAGCCTTCAACATGG NGG 2 0.1203 Tier III
25 NC_000069.6 3 130685416 + Gm36373 NNNGCCAGCCTTAAACGGGG NGG 2 0.0588 Tier III
26 NC_000081.6 15 51236813 + Gm19303 NNNGCCAGCCTTAAACATGT NGA 2 0.0486 Tier III
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Other clones with same target sequence:

(none)